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標題: 在無溶劑系統下以脂解酵素催化合成乙酸苯乙酯(玫瑰精油)之最佳化與動力學研究
Optimization and kinetic modeling of lipase catalyzed 2-phenylethyl acetate (rose essential oil) in a solvent-free system
作者: 陳玉旻
Chen, Yu-Min
關鍵字: 乙酸苯乙酯;2-phenethyl acetate;反應曲面法;動力學;無溶劑系統;玫瑰香味;RSM;kinetic;solvent-free;rose-like flavor
出版社: 化學系所
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香精與香料為在日成生活中為重要的添加劑,主要應用於食品、飼料、化妝品、化工、醫藥等行業,以及其他家用產品[1]。乙酸苯乙酯(2-phenethyl acetate,2-PEAc)為玫瑰花在開花時期,主要散發出的芳香物質[2]。乙酸苯乙酯為澄清淡黃色,味道類似於玫瑰花香,且常作用於香精與香料的添加劑。目前市面上大多數的乙酸苯乙酯皆是透過化學合成或是萃取所產生的。儘管如此,這些方法還是存在許多缺點,例如需在高溫或長時間底下反應、產生副產物、大量的廢水、刺鼻的臭味以及強酸腐蝕機器等問題。
在此實驗中,我們利用商業脂解酵素將苯乙醇(2-phenyl ethanol,2-PE)與乙酸乙酯(ethyl acetate,EA)在無溶劑系統底下催化合成乙酸苯乙酯[3]。 整個實驗分成批次式與連續式兩部分。在實驗的第一部分,為了找到合成乙酸苯乙酯之最佳化條件以及其動力學參數Km與Vmax,我們應用了五階二變中心混成實驗設計(Central Composite Design,CCD)和反應曲面法(Response Surface Methodology,RSM)來計算反應變數對整體反應的影響,其中包含反應溫度(45℃~65℃)以及苯乙醇濃度(100 mM~500 mM)在反應初速率、乙酸苯乙酯之莫耳轉化率以及其產量上的影響。基於RSM的分析,合成乙酸苯乙酯之最佳反應條件應為,反應溫度60.18℃以及苯乙醇濃度447.16 mM。在此條件下,反應初速率可達13.17 mM/min、在120分鐘時的轉化率為89.53 %,以及其產量為60.86 g/L。根據雙基質動力學反應,ping-pong bi-bi動力學模式以及RSM的數據,可計算出動力學參數,包括在反應溫度為55℃時之動力學參數Vmax (27.62 mM/min), Km (443.07 mM) 以及 Ki (414.85 mM)。
在實驗的第二部分,利用連續式酵素填充床生物反應器催化合成乙酸苯乙酯,在這部分是應用了三階三變Box-Behnken實驗設計(Box-Behnken design)方式合成乙酸苯乙酯,其中反應變數包含,苯乙醇濃度(100-500 mM)、反應流速(1-5 mL/min)及反應溫度(45-65 ℃),然後再以反應曲面法(response surface methodology,RSM)進行分析,分別探討合成反應參數對莫耳轉換率的影響。最後,利用脊型分析(analysis of ridge max)詳細探討酵素合成乙酸苯乙酯之最佳化反應條件。結果顯示,填充 10000 PLU固定化酵素NovozymR 435 於連續式酵素填充床生物反應器,合成乙酸苯乙酯之最佳化條件為:苯乙醇濃度62.007 mM、反應溫度 54.03 ℃及反應流速2.75 mL/min及,莫耳轉換率之理論值與實驗值分別為 100.22 %及 99.01±0.09 %。

Flavors’ and fragrances are important additive, mainly applied in food, feed, cosmetic, chemical, pharmaceutical industries, and other household products[1]. 2-phenylethyl acetate(2-PEAc) is a major emission of aromatic volatile ester from rose flowers, when the roses bloom[2]. 2-PEAc is colorless and smelling like sweet rose fragrance, and it generally used as an additive flavor and fragrances. Most of the 2-PEAc are now produced via chemical synthesis or extraction. However, chemical processes have several drawbacks, such as high/long reaction temperature/ time, side products, discharging wastewater, irritating smell, corrosiveness of machine etc…
In this study, we used commercial immobilized lipase to catalyze transesterification to synthesis 2-PEAc from 2-PE and ethyl acetate (EA) in a solvent-free system [3]. There are two parts in this research, one of parts is in a batch reactor and another part is in a continuous packed-bed bioreactor. In the first part, 5-level-2-factor central composite design (CCD) and response surface methodology (RSM) were employed to evaluate the effects of synthesis parameters, including reaction temperature (45~65 ℃), concentration of 2-PE (100~500 mM) on initial rate, yield of 2-PEAc, and productivity, in order to search the optimal reaction conditions and calculate the kinetic parameters, Vmax and Km. Based on the RSM analysis, the optimal conditions was found at reaction temperature of 60.18 ℃ and concentration of 2-PE 447.16 mM. The initial rate 13.17 mM/min, yield of 2-PEAc 89.53 %, and productivity 60.86 g/L were obtained under the optimal conditions. According to the ping-pong bi-bi kinetic model, the kinetic parameters, including Vmax (27.62 mM/min), Km (443.07 mM) and Ki (414.85 mM) were calculated form RSM data at 55 ℃. In the second part, a continuous packed-bed bioreactor was used for synthesis of 2-PEAc, 3-level-3-factor Box-Behnken design and response surface methodology (RSM) were employed to evaluate the effects of synthesis parameters, including concentration of 2-PE (100~500 mM), flow rate(1~5 mL/min) and reaction temperature (45~65 ℃). Based on ridge maximum analysis, we use the analysis of ridge maximum to search the optimal reaction conditions for synthesis of 2-PEAc were: concentration of 2-PE 62.07 mM, reaction temperature 54.03 ℃, and flow rate 2.75 mL/min. The molar conversion of predicted values and actual experimental values were 100.22 % and 99.01±0.09 % respectively.
其他識別: U0005-0108201202404800
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