Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/1527
標題: 旋轉微流道閥門可視化及雷射偵測
Visualization Experiments of Microfluidics Valve and Diode Laser Detection
作者: 黃柏鈞
Huang, Po-Chun
關鍵字: Centrifugal;離心力;Capillary valve;Diode laser;毛細閥;二極體雷射
出版社: 機械工程學系所
引用: [1] Thorsen, G., Ekstrand, G., Selditz, U., Wallenborg, R. S., and Andersson, P., “Integated Microfluidics for Parallel Processing of Proteins in a CD Microlaboratory,” Proceedings of 7th International Conference on Miniaturized Chemical and Biochemlcal Analysis Systems, Squaw Valley, California, USA, October 5-9, 2003. [2] Gustafsson, M., Hirschberg D., Palmberg, C., Jornvall, H., and Bergman, T., “Integrated Sample Preparation and MALDI Mass Spectrometry on a Microfluidic Compact Disk,” Analytical Chemistry, Vol. 76, NO.2, 2004, pp. 345-350. [3] Hirschberg, D., Jagerbrink, T., Samskog, J., Gustafsson, M., Stahlberg, M., Alvelius, G., Husman, B., Carlquist, M., Jornvall, H., and Bergman, T., “Detection of Phosphorylated Peptides in Proteomic Analyses Using Microfluidic Compact Disk Technology,” Analytical Chemistry, Vol. 76, 2004, pp. 5864-3871. [4] Kim, J., Jang S. H., Jia, G., Zoval, J. V., Silva N. A. D., and Madou M. 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J., Yao, T. J., and Tai, Y. C., “The Marching Velocity of the Capillary Meniscus in a Microchannel,” Joural of Micromechanics and Microengineering, Vol. 14, 2004, pp. 220-225. [13] Ducree, J., Brenner, T., Glatzel, T., and Zengerle, R., “A Coriolis-Based Split-and-Recombine Laminator for Ultrafast Mixing on Rotating Disks,” Proceedings of 7th International Conference on Miniaturized Chemical and Biochemlcal Analysis Systems, Squaw Valley, California, USA, October 5-9, 2003. [14] Roulet, J. C., Volkel, R., Herzig, H. P., Verpoorts, E., Derooij, N. F., and Dandiker R., “Performance of an Integrated Microoptical System for Fluorescence Detection in Microfluidic System,” Analytical Chemistry, Vol. 74, No.14, July 15, 2002, pp. 3400-3407. [15] Puckett, L. G., Dikici, E., Lai, S., Madou J. C., Bachas, L. G., and Daunert, S., “Investigation into the Applicability of the Centrifugal Microfluidics Platform for the Development of Protein-Ligand Binding Assays Incorporating Enhanced Green Fluorescent Protein as a Fluorescent Reporter,” Analytical Chemistry, Vol. 76, No.24, December 15, 2004, pp. 7263-7268. [16] Badr, I. H. A., Johnson, R. D., Madou, M. J., and Bachas, L. G., “Fluorescent Ion-Selective Optode Membranes Incorporated onto a Centrifugal Microfluidics Platform,” Analytical Chemistry, Vol. 74, No.21, November 1, 2002, pp. 5569-5575. [17] Hamamatsu; http://www.hamamatsu.com/, June 2006. [18] Varma, M. M., Nolte, D. D., Inerowicz, H. D., and Regnier, F. E., “Spinning-Disk Self-Referencing Interferometry of Antigen-Antibody Recognition,” Optical Society, Vol. 29, No. 9 , May 1, 2004. [19] Varma, M. M., Inerowicz, H. D., Regnier, F. E., and Nolte, D. D., “High-Speed Lable-Free Detection by Spinning-Disk Micro-Interferometry,” Biosensor and Bioelectronics, 2004, pp. 1371-1376. [20] Zeng, J., Deshpande M., Greiner, K. B., and Gilbert J. R., “Fluidic Capacitance Model of Capillary-Driven Stop Valves,” ASME international mechanical engineering congress and exposition, 2000 [21] Grumann, M., Brenner, T., Beer C., Zengerle, R., and Ducree, J., “Visualization of Flow Patterning in High-Speed Centrifugal Microfluidics,” Rev Sci Instru 76(2):025101s, 2005. [22] Duffy, D. C., Gills, H. L., Lin, J., Sheppard, N. F., and Kellogg, G. J., “Microfabricated Centrifugal Microfluidic Systems: Characterization and Multiple Enzymatic Assays,” Analytical Chemistry, Vol. 71, No. 20, 1999, pp. 4669–4678. [23] Man, P. F., Mastrangelo, C. H., Burns, M. A., and Burke, D. T., “Microfabricated Capillary-Driven Stop Valve and Sample Injector,” Proceedings of 11th Annual International Workshop on Micro Electro Mechanical Systems, Heidelberg, Germany, Jan. 25-29, 1998, pp. 45-50. [24] 林茂吉, 光碟式旋轉微流體混合之可視化實驗, 中興大學機械工程研究所碩士論文, 中華民國九十四年八月十八日。 [25] Lambert, P., Letier, P., and Delchambre, A., “Capillary and Surface Tension Force in the Manipulation of Small Parts,” Proceedings of the 5th IEEE Internation Symposium on Assembly and Task Planning, pp. 54-59 July 10-11, 2003. [26] Hecht, E., Optics, 4th edition, Addison Wesley, San Francisco, 2002, chapter 3 and 4. [27] 匯展電子; http://www.hueyjann.com.tw/Data/095.pdf, June 2006. [28] Zeng, J., Banerjee, D., Deshpande, M., Gilbert, J. R., Duffy, D. C., and Kellogg, G. J., “Design Analyses of Capillary Burst Valves in Centrifugal Microfluidics,” Technical Proceedings of Micro Total Analysis Systems, MicroTAS 2000, Enschede, The Netherlands, pp. 493–496.
摘要: 
本研究在碟盤式的壓克力基材上製作微流道,並觀察流體在微米級尺寸下流動的情形。為了控制流體啟始與停止運動,於流道上設計截面積突然改變的被動式閥門,研究中主要以三維毛細理論為設計基礎,並與量測結果相互驗證;實驗中觀察微流體受到離心力驅動的過程以及閥門使流體停止與啟動的技術。在較高轉速(2000轉以上)的微流體偵測,使用技術成熟的CD雷射讀寫頭,來取代CCD攝影。旋轉微流場的拍攝機制,利用步進馬達結合計數器的運用,完成旋轉中的微流場定位和觸發拍攝。閥門實驗的流道寬度為300、400μm深度為100至800μm,配合擴張角的變化,可在親水性的壓克力流道內製作被動式的閥門。實驗發現深寬比越大其突破轉速也較高;而同樣的尺寸擴張角越大突破轉速跟著變大,實驗的結果與理論的預期相當的一致。另外,雷射偵測機制可以準確的判別出流道內是否有流體,因為二極體雷射光路受到流體的干擾即有明顯的反應,然而顏色深淺與光路干擾相對關係,目前尚未得到確鑿的結果,仍需進一步研究。

This study uses the flow visualization method to observe capillary stop valves of red ink in the microchannel fabricated on a rotating compact disk (CD). The capillary valves presented here were designed according the 3D theory that takes account of the aspect ratio effect. It is found that the 3D analysis agrees well with the present experiments of the burst frequency while the 1D theory suffers a large deviation from the measurements for the case that the capillary channel has a low depth-to-width ratio.

The visualization mechanisms are composed of the image acquisition system, a step motor with function generator, and the in-house synchronizer for capturing the synchronizing images with the rotation motion. The passive valves for restraining the capillary force could be realized by designing a channel width of 300μm and 400μm together with a wedge angle ranging from 50° to 100°. The experimental results on the capillary-burst valves are consistent with the 3D theory.

Furthermore, the fluid existence in microchannel can be precisely detected by diode laser. The laser immediately evokes a response to the disturbing of the fluid, thus this result indicates the practicability of applying laser on flow switch detected regardless of the fluid color.
URI: http://hdl.handle.net/11455/1527
其他識別: U0005-0707200611094400
Appears in Collections:機械工程學系所

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