Please use this identifier to cite or link to this item:
|標題:||E. coli表現之PCV2 ORF2次單位疫苗之效益評估及PRRS病毒在協同引發PCV2相關疾病之角色
Evaluation of the Efficacy of E. coli Expressed PCV2 ORF2 Subunit Vaccine and Role of PRRS Virus on the Synergistic Development of PCV2-associated Disease
|關鍵字:||PCVAD;PCV2相關疾病;subunit vaccine;sugar cane extract;PRRSV;次單位疫苗;甘蔗萃取物||出版社:||獸醫病理生物學研究所||引用:||林佳蓉。豬環狀病毒第二型外殼蛋白之表現及應用。國立中興大學獸醫微生物學研究所。碩士論文。2004。 邱明堂，蘇保誠，莊孟修，林昭男。台灣地區罹病豬隻中第二型豬環狀病毒感染狀況之調查。台灣獸醫誌 30: 163-168, 2004。 楊程堯，張聰洲，林昭男，蔡敬屏，邱明堂。台灣地區豬呼吸道疾病綜合症相關病原之分析。台灣獸醫誌 33: 40-46, 2007 楊程堯，張聰洲，蘇秀琪，王佩玲，葉麗君，林嘉鈺，林怡絹，邱明堂。養豬場中第二型豬環狀病毒生物性傳播途徑之探討。台灣獸醫誌 33: 66-71, 2007。 蔡昇達。大腸桿菌表現之第二型豬環狀病毒次單位疫苗保護效益之評估: 田間試驗及人工感染試驗。國立中興大學獸醫病理學研究所。碩士論文。2007。 鄭宗興。利用血清及環境衛生之監測評估豬呼吸道疾病複合症與相關危險因子之關聯性。國立中興大學獸醫病理學研究所。碩士論文。2005。 Allan G, Meehan B, Todd D, Kennedy S, McNeilly F, Ellis J, Clark EG, Harding J, Espuna E, Botner A, Charreyre C. Novel porcine circoviruses from pigs with wasting disease syndromes. Vet Rec 142: 467-468, 1998. Allan GM, Ellis JA. Porcine circoviruses: a review. J Vet Diagn Invest 12: 3-14, 2000. Allan GM, Kennedy S, McNeilly F, Foster JC, Ellis JA, Krakowka SJ, Meehan BM. Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus. J Comp Pathol 121: 1-11, 1999. Allan GM, McNeilly F, McNair I, Curran MD, Walker I, Ellis JA, Konoby C, Kennedy S, Meehan BM. Absence of evidence for porcine circovirus type 2 in cattle and humans, and lack of seroconversion or lesions in experimentally infected sheep. Arch Virol 145: 853-857, 2000. Allan GM, McNeilly F, Ellis J, Krakowka S, Meehan B, McNair I, Walker I, Kennedy S. Experimental infection of colostrum deprived piglets with porcine circovirus 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) potentiates PCV2 replication. Arch Virol 145: 2421-2429, 2000. Allan GM, McNeilly F, Kennedy S, Meehan B, Ellis J, Krakowka S. Immunostimulation PCV-2 and PMWS. Vet Rec 5: 170-171, 2000. Allan GM, McNeilly F, McNair I, Armstrong D. The role of colostrum derived antibodies to PCV2 virus and vertical transmission of PCV2 in PMWS in the United Kingdom. (In preparation). 2004. Allan GM, McNeilly F, McNair I, Meehan B, Marshall M, Ellis J, Lasagna C, Boriosi G, Krakowka S, Reynaud G, Boeuf-Tedeschi L, Bublot M, Charreyre C. Passive transfer of maternal antibodies to PCV2 protects against development of postweaning multisystemic wasting syndrome (PMWS): Experimental infections and a field study. Pig J 50: 59-67, 2002. Allan GM, McNeilly F, Meehan BM, Kennedy S, Mackie DP, Ellis JA, Clark EG, Espuna E, Saubi N, Riera P, Botner A, Charreyre CE. Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland. Vet Microbiol 66: 115-123, 1999. Allan GM, Phenix KV, Todd D, McNulty MS. Some biological and physico-chemical properties of porcine circovirus. Zentralbl. Veterinarmed. 41: 17-26, 1994. Amer S, Na KJ, El-Abasy M, Motobu M, Koyama Y, Koge K, Hirota Y. Immunostimulating effects of sugar cane extract on X-ray radiation induced immunosuppression in the chicken. Int Immunopharmacol 4: 71-77, 2004. Amer S, Na KJ, Motobu M, El-Abasy M, Nakamura K, Koge K, Hirota Y. Radioprotective effect of sugar cane extract in chickens. Phytother Res 19: 496-500, 2005. Baebko P, Hassing AG, Olsen P, Lorenzen B, Wachmann H, Lauridsen C. Vitamin E and postweaning mortality in PMWS affected herds. Proc Congr Int Pig Vet Soc, pp. 62, 2004. Baker B. Diseases of the respiratory system. Proceedings of the North Carolina Healthy Hogs Seminar. 2005. Balasch M, Segales J, Rosell C, Domingo M, Mankerz A, Urniza M, Plana-Duran J. Experimental inoculation of conventional pigs with tissue homogenates from pigs with post-weaning multisystemic wasting syndrome. J Comp Pathol 121: 139-148, 1999. Bassaganya-Riera J, Pogranichniy RM, Jobgen SC, Halbur PG, Yoon KJ, O''Shea M, Mohede I, Hontecillas R. Conjugated linoleic acid ameliorates viral infectivity in a pig model of virally induced immunosuppression. J Nutr 133: 3204-3214, 2003. Benfield DA, Nelson E, Collins JE, Harris L, Goyal SM, Robison D, Christianson WT, Morrison RB. Characterization of swine infertility and respiratory syndrome (SIRS) virus ( isolate ATCC VR-2332). J Vet Diagn Invest 4: 127-133, 1992. Blanchard P, Loizel C, Baudouard MA, Nignol AC, Grasland B, Dory D, Morvan H, Cariolet R, Jestin A. Quantification du génome du circovirus porcin de type 2 (PCV2) par PCR en temps réel et corrélation avec la maladie d''amaigrissement du porcelet (MAP). Journ Rech Porcine France 36: 327–332, 2004. Blanchard P, Mahe D, Cariolet R, Keranflec''h A, Baudouard MA, Cordioli P, Albina E, Jestin A. Protection of swine against postweaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) protein. Vaccine 21: 4565-4575, 2003. Blanchard P, Mahe D, Cariolet R, Truong C, Le Dimna M, Arnauld C, Rose N, Eveno E, Albina E, Madec F, Jestin A. An ORF2 protein-based ELISA for porcine circovirus type 2 antibodies in post-weaning multisystemic wasting syndrome. Vet Microbiol 94(3): 183-194, 2003. Brunborg IM, Moldal T, Jonassen CM. Quantitation of PCV2 isolated from serum/plasma and tissue samples of healthy pigs and pigs with PMWS using a TaqMan-based real-time PCR. J Virol Methods 122: 171-178, 2004. Calsamiglia M, Segales J, Fraile L, Espinal A, Seminati C, Martin M, Mateu E, Domingo M. Sow effect on litter mortality in a swine integration system experiencing postweaning multisystemic wasting syndrome (PMWS). Vet J (submitted), 2004. Calsamiglia M, Fraile L, Espinal A, Cuxart A, Seminati C, Martin M, Mateu E, Domingo M, Segales J. Sow porcine circovirus type 2 status effect on litter mortality in postweaning multisystemic wasting syndrome. Res Vet Sci 82: 299-304, 2007. Caprioli A, McNeilly F, McNair I, Lagan-Tregaskis P, Ellis J, Krakowka S, McKillen J, Ostanello F, Allan G. PCR detection of porcine circovirus type 2 (PCV2) DNA in blood, tonsillar and faecal swabs from experimentally infected pigs. Res Vet Sci 81: 287-292, 2006. Chae C. Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Vet J 168: 41-49, 2004. Chae C. A review of porcine circovirus 2-associated syndromes and diseases. Vet J 169: 326-336, 2005. Chang HW, Jeng CR, Liu JJ, Lin TL, Chang CC, Chia MY, Tsai YC, Pang VF. Reduction of porcine reproductive and respiratory syndrome virus (PRRSV) infection in swine alveolar macrophages by PCV2-induced interferon-alpha. Vet Microbiol 108: 167-177. Chang HW, Jeng CR, Lin CM, Liu JJ, Chang CC, Tsai YC, Chia MY, Pang VF. The involvement of Fas/FasL interaction in porcine circovirus type 2 and porcine reproductive and respiratory syndrome virus co-inoculation-associated lymphocyte apoptosis in vitro. Vet Microbiol 122: 72-82, 2007. Chang HW, Pang VF, Chen LJ, Chia MY, Tsai YC, Jeng CR. Bacterial lipopolysaccharide induces porcine circovirus type 2 replication in swine alveolar macrophages. Vet Microbiol 115: 311-319, 2006. Chen SP, Weng YJ, Tsai CP, Chang WF. Case report: Identification of pathogenic porcine circovirus in pigs with post-weaning multisystemic wasting syndrome in Taiwan. J Chin Soc Vet Sci 27: 251-255, 2001. Cheung AK. Comparative analysis of the transcriptional patterns of pathogenic and nonpathogenic porcine circoviruses. Virology 310: 41-49, 2003. Chianini F, Majo N, Segales J, Dominguez J, Domingo M. Immunohistochemical characterisation of PCV2 associate lesions in lymphoid and non-lymphoid tissues of pigs with natural postweaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 94: 63-75, 2003. Chung WB, Chan WH, Chaung HC, Lien Y, Wu CC, Huang YL. Real-time PCR for quantitation of PRRS virus and PCV2 in naturally-infected and challenged pigs. J Virol Methods 124: 11-19, 2005. Clark EG. Post-weaning multisystemic wasting syndroms. Proc Am Assoc Swine Pract 28: 499-501, 1997. Cooper VL, Doster AR, Hesse RA, Harris NB. Porcine reproductive and respiratory syndrome: NEB-1 PRRSV infection did not potentiate bacterial pathogens. J Vet Diagn Invest 7: 313-320, 1995. Corrêa AM, Zlotowski P, de Barcellos DE, da Cruz CE, Driemeier D. Brain lesions in pigs affected with postweaning multisystemic wasting syndrome. J Vet Diagn Invest 19: 109-112, 2007. Darwich L, Pie S, Rovira A, Segales J, Domingo M, Oswald IP, Mateu E. Cytokine mRNA expression profiles in lymphoid tissues of pigs naturally affected by postweaning multisystemic wasting syndrome. J Gen Virol 84: 2117-2125, 2003. Darwich L, Segales J, Domingo M, Mateu E. Changes in CD4+, CD8+, CD4/ CD8 double positive cells and IgM+ cell subsets in peripheral blood mononuclear cells from PMWS affected pigs and age matched uninfected wasted and healthy pigs, correlates with lesions and PCV2 load in lymphoid tissues. Clin Diagn Lab Immunol 9: 236-242, 2002. Darwich L, Segalés J, Mateu E. Pathogenesis of postweaning multisystemic wasting syndrome caused by Porcine circovirus 2: An immune riddle. Arch Virol 149: 857-874, 2004. Donadeu M, Waddilove J, Marco E. European management strategies to control postweaning multisystemic wasting syndrome. Proc Allen D. Leman Swine Conf, pp. 136-142, 2003. Dorr PM, Baker RB, Almond GW, Wayne SR, Gebreyes WA. Epidemiologic assessment of porcine circovirus type 2 coinfection with other pathogens in swine. J Am Vet Med Assoc 230: 244-250, 2007. Drew TW. A review of evidence for immunosuppression due to porcine reproductive and respiratory syndrome virus. Vet Res 31: 27-39, 2000. Drolet R, Larochelle R, Morin M, Delisle B, Magar R. Detection rates of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, and swine influenza virus in porcine proliferative and necrotizing pneumonia. Vet Pathol 40: 143-148, 2003. El-Abasy M, Motobu M, Nakamura K, Koge K, Onodera T, Vainio O, Toivanen P, Hirota Y. Preventive and therapeutic effects of sugar cane extract on cyclophosphamide-induced immunosuppression in chickens. Int Immunopharmacol 4: 983-990, 2004. El-Abasy M, Motobu M, Na KJ, Shimura K, Nakamura K, Koge K, Onodera T, Hirota Y. Protective effects of sugar cane extracts on Eimeria tenella infection in chickens. J Vet Med Sci 65: 865-871, 2003a. El-Abasy M, Motobu M, Sameshima T, Koge K, Onodera T, Hirota Y. Adjuvant effects of sugar cane extract in chickens. J Vet Med Sci 65: 117-119, 2003b. El-Abasy M, Motobu M, Shimura K, Na KJ, Kang CB, Koge K, Onodera T, Hirota Y Immunostimulating and growth-promoting effects of sugar cane extracts in chickens. J Vet Med Sci 64: 1061-1063, 2002. Ellis JA, Bratanich A, Clark EG, Allan G, Meehan B, Haines DM, Harding J, West KH, Krakowka S, Konoby C, Hassard L, McNeilly F. Coinfection by porcine circoviruses and porcine parvovirus in pigs with naturally acquired postweaning multisystemic wasting syndrome. J Vet Diagn Invest 12: 21-27, 2000. Ellis J, Hassard L, Clark E, Harding J, Allan G, Wilson P, Strokappe J, Martin K, McNeilly F, Meehan B, Todd D, Haines D. Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndroms. Can Vet J 29: 44-51, 1998. Ellis J, Krakowka S, Allan G, Clark E, Kennedy S. The clinical scope of porcine reproductive and respiratory syndrome virus has expanded since 1987: an alternative perspective. Veterinary Pathology 36: 262–265, 1999. Ellis J, Krakowka S, Lairmore M, Haines D, Bratanich A, Clark E, Allan G, Konoby C, Hassard L, Meehan B, Martin K, Harding J, Kennedy S, McNeilly F. Reproduction of lesions of postweaning multisystemic wasting syndroms in gnotobiotic piglets. J Vet Diagn Ivest 11: 3-14, 1999. Fachinger V, Bischoff R, Jedidia SB, Saalmuller A, Elbers K. The effect of vaccination against porcine circovirus type 2 in pigs suffering from porcine respiratory disease complex. Vaccine 26: 1488-1499, 2008. Fenaux M, Halbur PG, Gill M, Toth TE, Meng XJ. Genetic characterization of type 2 porcine circovirus (PCV-2) from pigs with postweaning multisystemic wasting syndrome in different geographic regions of North America and development of a differential PCR-restriction fragment length polymorphism assay to detect and differentiate between infections with PCV-1 and PCV-2. J Clin Microbiol 38: 2494-2503, 2000. Fenaux M, Halbur PG, Haqshenas G, Royal R, Thomas P, Nawagitgul P, Gill M, Toth TE, Meng XJ. Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J Virol 76: 541-551, 2002. Fenaux M, Opriessnig T, Halbur PG, Elvinger F, Meng XJ. A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J Virol 78: 6297-6303, 2004. Fernandes LT, Mateu E, Sibila M, Fort M, Andaluz A, McNeilly F, Allan G, Sánchez A, Stevenson L. Lack of in vitro and in vivo effects of lipopolysaccharide on porcine circovirus type 2 infection. Viral Immunol 20: 541-552, 2007. Ferreira D, Sansot B, Laval A. Attempt to use serotherapy to control mortality in PMWS. Proc ssDNA Viruses Plants, Birds, Pigs and Primates (ESVV), p. 144, 2001. Fort M, Sibila M, Allepuz A, Mateu E, Roerink F, Segales J. Porcine circovirus type 2 vaccination of conventional pigs prevents viremia against PCV2 isolates of different genotypes and geographic origins. Vaccine 26: 1063-1071, 2008. Grasland B, Loizel C, Blanchard P, Oger A, Morvan H. Reproduction of PMWS in immunostimulated SPF piglets transfected with infectious cloned genomic DNA of type 2 porcine circovirus. Vet. Res 36: 685-697, 2005. Grau-Roma L, Segales J. Detection of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine influenza virus and Aujeszky’s disease virus in case of porcine proliferative and necrotizing pneumonia in Spain. Vet Microbiol 119: 144-151, 2007. Gregory W, Stevenson W, Kiupel M, Mittal SK, Choi J, Latimer KS, Kanitz CL. Tissue distribution and genetic typing of porcine circoviruses in pigs with naturally occurring congenital tremors. J Vet Diagn Invest 13: 57-62, 2001. Guilmoto H, Wessel-Robert S. Control of PMWS in Brittany: a mainly zootechnical approach. In PMWS: A new emerging disease of swine. Melbourne, Australia: Merial Symposium, pp. 45-55, 2000. Hamel AL, Lin LL, Nayar GP. Nucleotide sequence of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. J Virol 72: 5262-5267, 1998. Harding JC. Postweaning mutisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. Proc Am Assoc SW Prac 28 th Ann Meet Quebec Canada 28: 503, 1997. Harding JC. The clinical expression and emergence of porcine circovirus 2. Vet Microbiol 98: 131-135, 2004. Harms PA. Post-weaning multisystemic wasting syndrome-case investigations. In: Proc Seventh Annual Swine Disease Conference of Swine Practitioners, Iowa State University, Ames, Iowa, pp 43–47, 1999. Harms PA, Sorden SD, Halbur PG, Bolin SR, Lager KM, Morozov I, Paul PS. Experimental reproduction of severe disease in CD/CD pigs concurrently infected with type 2 porcine circovirus and porcine reproductive and respiratory syndrome virus. Vet Pathol 38: 528-539, 2001. Haruna J, Hanna P, Hurnik D, Ikede B, Miller L, Yason C. The role of immunostimulation in the development of postweaning multisystemic wasting syndrome in pigs under field conditions. Can J Vet Res 70: 269-276, 2006 Hassing AG, Kristensen CS, Baebko P, Wachmann. Effect of sow on the mortality of pigs after weaning in PMWS herds. Proc Congr Int Pig Vet Soc, pp. 76, 2004. Hasslung FC, Berg M, Allan GM, Meehan BM, McNeilly F, Fossum C. Identification of a sequence from the genome of PCV2 with an inhibitory effect on IFN-α production by porcine PBMCs. J Gene Virol 84: 2937-2945, 2003. Hikosaka K, El-Abasy M, Koyama Y, Motobu M, Koge K, Isobe T, Kang CB, Hayashidani H, Onodera T, Wang PC, Matsumura M, Hirota Y. Immunostimulating effects of the polyphenol-rich fraction of sugar cane extract in chickens. Phytother Res 21: 120-125, 2007. Hino S, Miyata H. Torque teno virus (TTV): current status. Rev Med Virol 17: 45-57, 2007. Horlen KP, Dritz SS, Nietfeld JC, Henry SC, Hesse RA, Oberst R, Hays M, Anderson J, Rowland RR. A field evaluation of mortality rate and growth psrformance in pigs vaccinated against porcine circovirus type 2. J Am Vet Med Assoc 232: 906-912, 2008. Irshad M, Joshi YK, Sharma Y, Dhar I. Transfusion transmitted virus: A review on its molecular characteristics and role in medicine. World J Gastroenterol 12: 5122-5134, 2006. Johnson CS, Joo HS, Direksin K, Yoon KJ, Choi YK. Experimental in utero inoculation of late-term swine fetuses with porcine circovirus type 2. J Vet Diagn Invest 14: 507-512, 2002. Kawashima K, Katsuda K, Tsunemitsu H. Epidemiological investigation of the prevalence and features of postweaning multisystemic wasting syndrome in Japan. J Vet Diagn Invest 19: 60-68, 2007. Kawashima K, Tsunemitsu H, Horino R. Effects of dexamethasone on the pathogenesis of PCV2 infection in piglets. J Comp Pathol 129: 294-302, 2003. Kim J, Chung HK, Chae C. Association of porcine circovirus 2 with porcine respiratory disease complex. Vet J 166: 251-256, 2003. Kim J, Chung HK, Jung T, Cho WS, Choi C, Chae C. Postweaning nultisystemic wasting syndrome of pigs in Korea: prevalence, microscopic lesions and coexisting microorganism. J Vet Med Sci 64: 57-62, 2002. Kim J, Chae C. A comparison of virus isolation, polymerase chain reaction, immunohistochemistry, and in situ hybridization for the detection of porcine circovirus 2 and porcine parvovirus in experimentally and naturally coinfected pigs. J Vet Diagn Invest 16: 45–50, 2004. Kim J, Ha Y, Chae C. Potentiation of porcine circovirus 2-induced postweaning multisystemic wasting syndrome by porcine parvovirus is associated with excessive production of tumor necrosis factor-α. Vet Pathol 43: 718-725, 2006. Kim J, Han DU, Choi C, Chae C. Simultaneous detection and differentiation between porcine circovirus and porcine parvovirus in boar semen by multiplex seminested PCR. J Vet Med Sci 65: 741-744, 2003. Kixmoller M, Ritzmann M, Eddicks M, Saalmuller A, Elbers K, Fachinger V. Reduction of PMWS-associated clinical signs and co-infections by vaccination against PCV2. Vaccine 26: 3443-3451, 2008. Koge K, Nagai Y, Mizutani T, Suzuki M, Araki S. Inhibitory effects of sugar cane extracts on liver injuries in mice. Nippon Shokuhin Kagaku Kogaku Kaishi 48: 231-237, 2001. Krakowka S, Ellis JA, McNeilly F. Immunologic features of porcine circovirus type 2 infection. Viral Immunol 15: 567-582. Krakowka S, Ellis JA, McNeilly F. Mycoplasma hyopneumoniae bacterins and porcine circovirus type 2 infection: Induction of postweaning multisystemic wasting syndrome in the gnotobiotic swine model of PCV2-associated disease. Can Vet J 48:716-724, 2007. Ladekjaer-Mikkelsen AS, Nielsen J, Stadejek T. Transplacenntal infection with PCV2 associated with reproductive failure in a gilt. Vet Rec 148: 759-760, 2001. Ladekjaer-Mikkelsen AS, Nielsen J, Stadejek T, Storgaard T, Krakowka S, Ellis J, McNeilly F, Allan G, Botner A. Reproduction of PMWS in immunostimulated and non-immunostimulated 3-week-old piglets experimentally infected with PCV2. Vet Microbiol 89: 97-114, 2002. Larochelle R, Bielanski A, Müller P, Magar R. PCR detection and evidence of shedding of porcine circovirus type 2 in boar semen. J Clin Microbiol 38: 4629-32, 2000. Lin CM, Jeng CR, Chang HW, Guo IC, Huang YL, Tsai YC, Chia MY, Pang VF. Characterization of PCV2 infection in swine lymphocytes using mitogen-stimulated peripheral blood lymphocytes from healthy PCV2-carrier pigs. Vet Immunol Immunopathol 124: 355-366, 2008. Liu Q, Wang L, Willson P, Babiuk LA. Quantitative, competitive PCR analysis of porcine circovirus DNA in serum from pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol 38: 3474-3477, 2000. Lo DY, Chen TH, Chien MS, Koge K, Hosono A, Kaminogawa S, Lee WC. Effects of sugar cane extract on the modulation of immunity in pigs. J Vet Med Sci 67: 591-597, 2005. Lo DY, Chien MS, Yeh KS, Koge K, Lin CC, Hsuan SL, Lee WC. Effects of sugar cane extract on pseudorabies virus challenge of pigs. J Vet Med Sci 68: 219-225, 2006. Lukert PD, de Boer GF, Dale JL, Keese P, McNulty MS, Randles JW, Tischer I. Family circoviridae. In "virus taxonomy. Classification and nomenclature of viruses" (Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD), Sixth report of the International Committee of Taxonomy for viruses. 166-168, 1995. Mateu E, Diaz I. The challenge of PRRS immunology. Vet J 177: 345-351, 2008. Madec F, Eveno E, Morvan P, Hamon L, Blanchard P, Cariolet R, Amenna N, Morvan H, Truong C, Mahe D, Albina E, Jestin A. Post-weaning multisystemic wasting syndrome (PMWS) in pigs in France: Clinical observations from follow-up studies on affected farms. Livest Prod Sci 63: 223-233, 2000. Madec F, Rose N, Grasland B, Cariolet R, Jestin A. Post-weaning multisystemic wasting syndrome and other PCV2-related problems in pigs: a 12-year experience. Transbound Emerg Dis 55: 273-283, 2008. Magar R, Larochelle R, Thibault S, Lamontagne. Experimental transmission of porcine circovirus type 2 (PCV2) in weaned pigs: a sequential study. J Comp Pathol 123: 258-269, 2000. Magar R, Muller P, Larochelle R. Retrospective serological survey of antibodies to porcine circovirus type 1 and type 2. Can J Vet Res 64: 184-186, 2000. Mahe D, Blanchard P, Truong C, Arnauld C, Le Cann P, Cariolet R, Madec F, Albina E, Jestin A. Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes. J Gen Virol 81: 1815-1824, 2000. Mankertz A, Mankertz J, Wolf K, Buhk HJ. Identification of a protein essential for replication od porcine circovirus. J Gen Virol. 79: 381-384, 1998. Mankertz A, Persson F, Mankertz J, Blaess G, Buhk HJ. Mapping and characterization of the origin of DNA replication of porcine circovirus. J Virol 71: 2562-2566, 1997. McKeown NE, Opriessnig T, Thomas P, Guenette DK, Elvinger F, Fenaux M, Halbur PG, Meng XJ. Effects of PCV2 maternal antibodies on experimental infection of piglets with PCV2. Clin Diagn Lab Immunol 12: 1347-1351, 2005. McNeilly F, Kenedy S, Moffett D, Meehan BM, Foster JC, Clarke EG, Ellis JA, Haines DM, Adair BM, Allan GM. A comparison of in situ hybridization and immunohistochemistry for the detection of a new porcine circovirus in formalin-fixed tissues from pigs with post-weaning multisystemic wasting syndrome (PMWS). J Virol Methods 80: 123-128, 1999. McNeilly F, McNair I, Mackie DP, Meehan BM, Kennedy S, Moffett D, Ellis J, Krakowka S, Allan GM. Production, characterisation and applications of monoclonal antibodies to porcine circovirus 2. Arch Virol 146: 909-922, 2001. Meehan BM, McNeilly F, Todd D, Kennedy S, Jewhurst VA, Ellis JA, Hassard LE, Clark EG, Haines DM, Allan GM. Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J Gen Virol 79: 2171-2179, 1998. Meerts P, Misinzo G, McNeilly F, Nauwynck HJ. Replication kinetics of different porcine circovirus 2 strains in PK-15 cells, fetal cardiomyocytes and macrophages. Arch Virol 150: 427-441, 2005. Misinzo G, Meerts P, Bublot M, Mast J, Weingartl HM, Nauwynck HJ. Binding and entry characteristics of porcine circovirus 2 in cells of the porcine monocytic line 3D4/31. J Gen Virol 86: 2057-2068, 2005. Morin M, Girard C, ElAzhary Y, Fajardo R, Drolet R, Lagacé A. Severe proliferative and necrotizing pneumonia in pigs: a newly recognized disease. Can Vet J 31: 837-839, 1990. Morozov I, Sirinarumitr T, Sorden SD, Halbur PG, Morgan MK, Yoon KJ, Paul PS. Detection of a novel strain of porcine circovirus in pigs with postweaning multisystemic wasting syndrome. J Clin Microbiol 36: 2535-2541, 1998. Motobu M, Amer S, Koyama Y, Hikosaka K, Sameshima T, Yamada M, Nakamura K, Koge K, Kang CB, Hayasidani H, Hirota Y. Protective effects of sugar cane extract on endotoxic shock in mice. Phytother Res 20: 359-363, 2006. Naasani I, Oh-Hashi F, Oh-Hara T, Feng WY, Johnston J, Chan K, Tsuruo T. Blocking telomerase by dietary polyphenols is a major mechanism for limiting the growth of human cancer cells in vitro and in vivo. Cancer Res 63: 824-830, 2003. Nawagitgul P, Morozov I, Bolin SR, Harms PA, Sorden SD, Paul PS. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein. J Gen Virol 81: 2281-2287, 2000. Nielsen J, Vincent IE, Botner A, Ladekaer-Mikkelsen AS, Allan G, Summerfield A, McCullough KC. Association of lymphopenia with porcine circovirus type 2 induced postweaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 92: 97-111, 2003. O''Connor B, Gauvreau H, West K, Bogdan J, Ayroud M, Clark EG, Konoby C, Allan G, Ellis JA. Multiple porcine circovirus 2-associated abortions and reproductive failure in a multisite swine production unit. Can Vet J 42: 551-553, 2001. Olvera A, Sibila M, Calsamiglia M, Segales J, Domingo M. Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigs. J Virol Methods 117: 75-80, 2004. Opriessnig T, Meng XJ, Halbur PG. Porcine circovirus type 2 associated disease: update on current terminology, clinical manifestations, pathogenesis, diagnosis, and intervention strategies. J Vet Diagn Invest 19: 591-615, 2007. Opriessnig T, Madson DM, Prickett JR, Kuhar D, Lunney JK, Elsener J, Halbur PG. Effect of porcine circovirus type 2 vaccination on PRRSV and PCV2 coinfection. Vet Microbiol 131: 103-114, 2008. Opriessnig T, Thacker E, Halbur PG. Proc 19th International Pig Veterinary Society Congress 2: 302, 2006. Opriessnig T, Thacker E, Yu S, Fenaux M, Meng XJ, Halbur PG. Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2. Vet Pathol 41: 624-640, 2004. Pensaert MB, Sanchez RE, Ladekjaer-Mikkelsen AS, Allan GM, Mauwynck HJ. Viremia and effect of fetal infection with porcine viruses with special reference to porcine circovirus 2 infection. Vet Microbiol 98: 175-183, 2004. Pogranichniy R, Yoon KJ, Yaeger M, Vaughn E, Stammer R, Roof M. Efficacy of experimental inactivated PCV2 vaccines for preventing PMWS in CDCD pigs. Proc Annu Meet Am Assoc Swine Vet, pp. 443-444, 2004. Quintana J, Segales J, Rosell C, Calsamiglia M, Rodriguez-Arrioja GM, Chianini F, Folch JM, Maldonado J, Canal M, Plana-Duran J, Domingo M. Clinical and pathological observations on pigs with postweaning multisystemic syndrome. Vet Rec 149: 357-361, 2001. Rodriguez-Arrioja GM, Segales J, Balasch M, Rosell C, Quintant J, Folch JM, Plana-Duran J, Mankertz A, Domingo M. Serum antibodies to porcine circovirus type 1 and type 2 in pigs with and without PMWS. Vet Rec 146: 762-764, 2000. Rodriguez-Arrioja GM, Segales J, Calsamiglia M, Resendes AR, Balasch M, Plana-Duran J, Casal J, Domingo M. Dynamics of porcine circovirus type 2 infection in a herd of pigs with postweaning multisystemic wasting syndrome. Am J Vet Res 63: 354-357, 2002. Rosell C, Segalés J, Domingo M. Hepatitis and staging of hepatic damage in pigs naturally infected with porcine circovirus type 2. Vet Pathol 37: 687–692, 2000. Rosell C, Segales J, Plana-Duran J, Balasch M, Rodriguez-Arrioja GM, Kennedy S, Allan GM, McNeilly F, Latimer KS, Domingo M. Pathological, immunohistochemical and in situ hybridization studies of natural cases of postweaning multisystemic wasting syndrome (PMWS) in pigs. J Comp Pathol 120: 59-78, 1999. Rosell C, Segales J, Ramos-Vara JA, Folch JM, Rodriguez-Arrioja GM, Duran CO, Balasch M, Plana-Duran J, Domingo M. Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome. Vet Rec 146: 40-43, 2000. Rovira A, Balasch M, Segales J, Garcia L, Plana-Duran J, Rosell C, Ellerbrock H, Mankertz A, Domingo M. Experimental inoculation of conventional pigs with porcine reproductive and respiratory syndrome virus and porcine circovirus 2. J Virol 76: 3232-3239, 2002. Royer RL, Nawagitgul P, Halbur PG, Paul PS. Susceptibility of porcine circovirus type 2 to commercial and laboratory disinfectants. J Swine Health Prod 9: 281-284, 2001. Sanchez RE Jr, Meerts P, Nauwynck HJ, Pensaert MB. Change of porcine circovirus 2 target cells in pigs during development from fetal to early postnatal life. Vet Microbiol 95: 15-25, 2003. Sanchez RE Jr, Nauwynck HJ, McNeilly F, Allan GM, Pensaert MB. Porcine circovirus 2 infection in swine foetuses inoculated at different stages of gestation. Vet Microbiol 83: 169-176, 2001. Sarli G, Mandrioli L, Laurenti M, Sidoli L, Cerati C, Rolla G, Marcato PS. Immunohistochemical characterisation of the lymph node reaction in pig post-weaning multisystemic wasting syndrome (PMWS). Vet Immunol Immunopathol 83: 53-67, 2001. Segales J, Alonso F, Rosell C, Pastor J, Chianini F, Campos E, Lopez-Fuertes L, Quintana J, Rodriguez-Arrioja G, Calsamiglia M, Pujols J, Dominguez J, Domingo M. Changes in peripheral blood leukocyte populations in pigs with natural PMWS. Vet Immunol Immunopathol 81: 37-44, 2001. Segales J, Calsamiglia M, Olvera A, Sibila M, Badiella L, Domingo M. Quantification of PCV2 DNA in serum and tonsillar, nasal, tracheo-bronchial, urinary and faecal swabs of pigs with and without PMWS. Vet Microbiol 111: 223-229, 2005. Segales J, Domingo M. Postweaning multisystemic wasting syndrome (PMWS) in pigs. A review. Vet Q 24: 109-124, 2002. Segales J, Domingo M, Chianini F, Majo N, Dominguez J, Darwich L, Mateu E. Immunosuppression in postweaning multisystemic wasting syndrome affected pigs. Vet Microbiol 98: 151-158, 2004. Segales J, Rosell C, Domingo M. Pathological findings associated with naturally acquired porcine circovirus type 2 associated disease. Vet Microbiol 98: 137-149, 2004. Segalés J, Rosell C, Rodr´ıguez-Arrioja G, Domingo M. Patolog´ıa de la circovirosis porcina. Porci 56: 41–54, 2000. Shi K, Li H, Guo X, Ge X, Jia H, Zheng S, Yang H. Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Vet Microbiol 129: 367-377, 2008. Shibata I, Okuda Y, Kitajima K, Asai T. Shedding of porcine circovirus into colostrum of sows. J Vet Med B Infect Dis Vet Public Health 53: 278-280, 2006. Shibata I, Okuda Y, Yazawa S, Ono M, Sasaki T, Itagaki M, Nakajima N, Okabe Y, Hidejima I. PCR detection of porcine circovirus type 2 DNA in whole blood, serum, oropharyngeal swab, nasal swab, and feces from experimentally infected pigs and field cases. J Vet Med Sci 65: 405-408, 2003. Shibahara T, Sato K, Ishikawa Y, Kadota K. Porcine circovirus induces B lymphocyte depletion in pigs with wasting disease syndrome. J Vet Med Sci 62: 1125-1131, 2000. Smith WJ, Thomson JR, Done S. Dermatitis/nephropathy syndrome of pigs. Vet Rec 132: 47, 1993 Takara K, Matsui D, Wada K, Ichiba T, Nakasone Y. New antioxidative phenolic glycosides isolated from Kokuto non-centrifuged cane sugar. Biosci Biotechnol Biochem 66: 29-35, 2002. Tischer I, Bode L, Apodaca J, Timm H, Peters D, Rasch R, Pociuli S, Gerike E. Presence of antibodies reacting with porcine circovirus in sera of humans, mice, and cattle. Arch Virol 140:1427-1439, 1995. Tischer I, Bode L, Peters D, Pociuli S, Germann B. Distribution of antibodies to porcine circovirus in swine populations of different breeding farms. Arch Virol 140: 737-743, 1995. Tischer I, Gelderblom H, Vettermann W, Koch MA. A very small porcine virus with a circular single-strand DNA. Nature 295: 64-66, 1982. Tischer I, Peters D, Rasch R, Pociuli S. Replication of porcine circovirus: induction by glucosamine and cell cycle dependence. Arch Virol 96: 39-57, 1987. Tischer I, Rasch R, Tochtermann G. Characterization of papovavirus and picornavirus-like particles in permanent pig kidney cell lines. Zentralba Bakteriol Org A 226: 153-167, 1974. Walker IW, Konoby CA, Jewhurst VA, McNair I, McNeilly F, Meehan BM, Cottrell TS, Ellis JA, Allan GM. Development and application of a competitive enzyme-linked immunosorbent assay for the detection of serum antibodies to porcine circovirus type 2. J Vet Diagn Invest 12: 400-405, 2000. Wang C, Huang TS, Huang CC, Tu C. Jong MH, Lin SY, Lai SS. Characterization of porcine circovirus type 2 in Taiwan. J Vet Med Sci 66: 469-475, 2004. Wang X, Eaton M, Mayer M, Li H, He D, Nelson E, Christopher-Hennings J. Porcine reproductive and respiratory syndrome virus productively infects monocyte-derived dendritic cells and compromises their antigen-presenting ability. Arch Virol 152: 289-303, 2007. Wang X, Jiang W, Jiang P, Li Y, Feng Z, Xu J. Construction and immunogenicity of recombinant adenovirus expressing the capsi||摘要:||
豬離乳後多系統性消耗性症候群(postweaning multisystemic wasting syndrome, PMWS)亦稱豬環狀病毒相關疾病(porcine circovirus associated disease, PCVAD)，為近年來一重要新興豬病，其發生與PCV2 (porcine circovirus type 2)感染有關，又常與其他病原如PRRSV (porcine reproductive and respiratory syndrome virus)混合感染導致更嚴重之病情。有關PCVAD之防治除落實高度衛生管理外，疫苗的使用被認為是有效策略，而甘蔗萃取物(sugar cane extract, SCE)為一種天然產物，具有免疫調節功能，有研究顯示可降低PRRSV抗體轉陽率及病毒核酸之陽性檢出率，認為具降低PRRSV之感染及散佈。本試驗主要目的在評估以改良後大腸桿菌表現之PCV2/ORF2所製作之次單位疫苗之保護效益。試驗一豬隻以3週離乳隔離飼養，以降低PRRSV自然感染方式及以人工感染PCV2來探討疫苗保護效益，並同步觀察移行抗體高低程度對PCV2感染之影響。試驗豬隻於3及6週齡時進行免疫，並於9週齡時進行PCV2病毒接種，以臨床病徵、生長性能、病理變化、血清抗體及PCV2病毒量表現等來評估疫苗保護效益。結果所有試驗豬並無明顯PRRSV感染，在PCV2接種後試驗豬隻無明顯臨床病徵，呼吸道相關病變也極為輕微。免疫組在PCV2接種後第2及第3週平均發燒天數(0.33及0天)略低於未免疫組(0.5及1.67天)；日增重(0.58±0.10 kg/day)則略高於無免疫組(0.51±0.10 kg/day)。淋巴組織病毒量方面，免疫組與無免疫組間並無顯著性差異，但遠高於5週齡有較高移行抗體之感染組。顯示疫苗免疫僅呈輕度保護作用及效益，此外，較高之移行抗體對豬群具有較高之保護作用。試驗二則選取4週齡離乳，但未隔離飼養之商業用豬並讓豬群自然曝露於PRRSV感染，並以相同模式再次評估疫苗保護效益外，另設2組分別於攻毒前或攻毒時給予免疫調節物質SCE，以觀察SCE對PRRSV或PCV2感染之影響。結果大部分豬隻於試驗前或試驗期間已有PRRSV感染現象(血清100%轉陽)，在 PCV2接種後，亦可誘發相當程度之呼吸道病變，並導致部份免疫豬隻發病死亡，無法呈現疫苗保護作用及效益。然而給予SCE的豬隻其臨床表現及病變均較陽性對照組為輕微，淋巴組織及血漿的病毒量也較為低，推測SCE可提升豬隻免疫力進而降低PCV2之感染。以上結果顯示，在PCV2與PRRSV共同感染時，的確可誘發相當程度之臨床症狀與呼吸道病變，而疫苗之保護效益則尚待進一步試驗與評估。以上研究顯示，PCVAD的防治上首在落實良好衛生管理，亦可考慮免疫母豬提高移行抗體力價以提供較多的保護作用，或於適當時期餵飼SCE以提升豬隻先天性免疫來控制PCVAD。
Postweaning multisystemic wasting syndrome (PMWS), also named as porcine circovirus associated disease (PCVAD), is an important emerging disease in swine in recent years. Porcine circovirus type 2 (PCV2) has been demonstrated to be the cause associated with PMWS. PCV2 co-infection with other pathogen, i.e. porcine reproductive and respiratory syndrome virus (PRRSV), is common and exacerbates the severity of PMWS. In addition to the implement of high level of biosecurity of farms, the use of the PCV2 vaccine is an effective strategy to control PCVAD. Sugar cane extract (SCE) is a native immunomodulator and can reduce the severity of infection. The objective of the present study was to evaluate the efficacy of the novel E. coli expressed PCV2/ORF2 subunit vaccines. The first experiment we evaluated the efficacy of PCV2 vaccines on conventional pigs under experimental control with less PRRSV exposure and the effects of PCV2 maternal antibodies on pigs experimentally infected with PCV2. Experimental pigs received double vaccinations of PCV2 subunit vaccine at 3 and 6 weeks of age, and then were inoculated with PCV2 inocula at 9 weeks of age. The clinical symptoms, pathological lesions, PCV2 loading and PCV2 antibody profile were evaluated. The results displayed that all experimental pigs after PCV2 inoculation presented mild clinical signs and respiratory lesions. Mean days of fever during 4 weeks inoculation after PCV2 challenge were slightly lower in vaccination group than in non-vaccination group. In contrast, mean daily weight gain were slightly higher in vaccination group (0.580.10 kg/day) than in non-vaccination group (0.510.10 kg/day). However, there was no significant difference in PCV2 loading between vaccination and non-vaccination group, but was significantly higher than PCV2 inoculated group at 5 weeks of age. Experiments above shows mild efficacy of the novel PCV2 subunit vaccine on these vaccinated pigs and high maternal antibodies offer more protection for piglets. The second experiment the evaluation of the efficacy of vaccines was repeated, that experimental pigs were naturally exposure to PRRSV infection. Moreover, effects of SCE on PRRSV or PCV2 infection were also included. The results displayed that most pigs suffered from PRRSV and PCV2 co-infection with more severe clinical signs and pulmonary lesions. The efficacy of the PCV2 vaccine on vaccinated pigs was not supported under PRRSV and PCV2 co-infection. However, pigs fed with SCE presented milder clinical signs, lesions, and PCV2 load in tissues. Therefore, SCE administration might have an immunostimulating effects on porcine immunity to reduce the pulmonary impairment against PCV2 and PRRSV co-infection. Taken together, co-infections with PCV2 and PRRSV can induce severe pulmonary lesions and clinical signs. The efficacy of the novel PCV2 subunit vaccine remains further elucidation. With the implement of high level of hygiene, increasing maternal antibody to confer more protection to piglets by means of boosting sows with PCV2 antigen, and feeding SCE in appropriate time, could be considered as another strategy in the control of PCVAD.
|Appears in Collections:||獸醫病理生物學所|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.