Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/1629
標題: 網版印刷電泳-電化學晶片測試
Electrochemical Detection Using Screen-Printed Electrodes in Microchip
作者: 簡煜修
Chien, I-Hsiu
關鍵字: electrophoresis;電泳;electrochemical detection;FEMLAB;電化學;檢測;FEMLAB
出版社: 機械工程學系所
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摘要: 
本研究主要是在探討微流體晶片中,毛細電泳效應對神經傳導物質dopamine與catechol的分離效果,並搭配電化學檢測方式來對分離後的的目標檢體做偵測。
在晶片的設計上,本文利用有限元素分析軟體FEMLAB預先模擬微流場中,檢體樣本的移動情形以及擴散的狀況,並藉此模擬的結果作為晶片電化學電極設計的依據。
在晶片的製造上,結合微影製程技術、網版印刷技術、熱壓接合技術製作出以碳作為電極材料、厚膜光阻JSR為結構的複合式微流體晶片。
測試結果發現,自製複合式微流體晶片成功的分離了dopamine與catechol,並且搭配電化學檢測技術成功的偵測到了分離後的檢體訊號。在100V到400V的外加電壓範圍內,檢體樣本的移動速度與外加電壓大致呈現線性關係。

The goal of this study is to use the capillary electrophoresis effect to separate the neurotransmitters dopamine and catechol on the self-made hybrid micro-fluidic chip which integrated with electrochemical detection(ECD) electrode to detect the signal of separated sample.
At first, we use finite element analysis software: FEMLAB to simulate the electrokinetic flow condition and the substances migration under the electrokinetic force in the microchannel. According to the computer simulation results, we can design the shape and the position of the electrochemical electrodes.
Thereafter, we address the micro fabrication process of the chip and check the performance of the completed chips.
The tests show that the self-made micro-fluidic chips had been successfully in separation of dopamine and catechol in an effective manner by contrast with traditional capillary electrophoresis. Furthermore, the ECD techniques had been well integrated with capillary electrophoresis on the micro-fluidic chip, too
URI: http://hdl.handle.net/11455/1629
其他識別: U0005-1807200615121000
Appears in Collections:機械工程學系所

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