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標題: 利用微奈米環境提升免疫檢測效果
Enhancing sensitivities of immunoassays by nanoenvironment
作者: 陳彥廷
Chen, Yen-Ting
關鍵字: 3D gold nano particle seneor;3D奈米金顆粒感測器;serpentine microfludic channel;Der p2;S型微流裝置;歐洲式塵螨
出版社: 機械工程學系所
引用: [1] Thomas WR, Smith WA, Hales BJ, Mills KL, O''Brien RM: Characterizationand immunobiology of house dust mite allergens. Int Arch Allergy Immunol 2002, 129(1):1-18. [2] J. J. Tsai, H. D. Shen, K. Y. Chua, Purification of group 2 Dermatophagoides pteronyssinus allergen and prevalence of its specific IgE in asthmatics, Int Arch Allergy Immunol, 121(3), 205-210, 2000. [3] J. J. Tsai, J. Y. Yen, Y. H. Yang, The Prevalence of Der p 2 Allergy in Asthmatic Patients in Taiwan. 2nd Congress of the Federation of Immunological Societies of Asia-Oceania, 129-35, 2000. [4] E. C. Liao, E. L. Hsu, J. J. Tsai, C. M. Ho, Immunologic Characterization and Allergenicity of Recombinant Tyr p 3 Allergen from the Storage Mite Tyrophagus putrescentiae, Int Arch Allergy Asthma Innumol. (IN press) [5] E. C. Liao, C. M. Ho, J. J. Tsai, The Prevalence of Tyrophagus putrescentiae Hypersensitivity and its Clinical Relevance in the Geriatrics. (IN submit) [6] Y. M. Chiung, C. W. Yao, J. J. Tsai, C. J. Chen, T. S. Shih, An Investigation of Hypersensitive Disease and Identification of Workers Exposed to Isocynates, J Occupational Safety and Health, 13, 129-138, 2005. [7] Y. H. Liu and J. J. Tsai, Production of salivary immunoglobulin A and suppression of Dermatophagoides pteronyssinus-induced airway inflammation by local nasal immunotherapy, Int Arch Allergy Immunol, 138(2), 161-8, 2005. [8] J. J. Tsai, W. C. Chen, Relation of different food allergens to different age of asthmatic patients, J Microbiol Immunol Infect, 32, 47-51, 1999. [9] E. Engvall, P. Perlman, “Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G,” Immunochemistry, v8, pp.871-874, 1971. [10] Hirsch, L. R.; Jackson, J. B.; Lee, A.; Halas, N. J.; West, J. Anal.Chem. 2003, 75, 2377. [11] J. J. Tsai, W. C. Chen, Relation of different food allergens to different age of asthmatic patients, J Microbiol Immunol Infect, 32, 47-51, 1999. [12] W. C. Bigelow, D. L. Pickett, W. A. J. Zisman, Colloid Interface Sci., 1, 513, 1946. [13] R. G. Nuzzo, F. A. Fusco, D. L. Allara, Spontaneously organized molecular assemblies preparation and properties of solution adsorbed monolayers of organic disulfides on gold surfaces, J. of the American Chemical Society, 109, 2358-2368, 1987. [14] E. S. Rubinstein, R. Maoz, and J. Sagiv, Organized monolayers on gold electrodes in electrochemical sensors for biomedical Applications, The Electrochemical Society, 175, 1986. [15] L. H. Dubois and R. G.. Nuzzo, Synthesis, structure, and properties of model organic surfaces, Annu. Rev. Phys. Chem, 43, 437-463, 1992. [16] W. R. Everett, T. L. Welch, L. Reed, I. F. Faules, Potential-dependent stability of self-assembled organothiols on gold electrodes in methylene chloride, Analytical Chemistry, 67, 292-298, 1995. [17] Zhong, Li; Zhang, Wei; Zer, Cindy; Ge, Kun; Gao, Xu; Kernstine, Kemp H, Protein microarray: Sensitive and effective immunodetection for drug residues, BMC Biotechnology, v 10, February 16, 2010 [18] Yanbin Li, Xiao-Li Su, “Microfluidics-Based Optical Biosensing Method for Rapid Detection of Escherichia Coli O157:H7,” Journal of Rapid Methods and Automation in Microbiology, v14, pp.96-109, 2006. [19] E. Eteshol, D. Leckband, “Development and characterization of an ELISA assay in PDMS microfluidic channels,” Sensors and Actuators B , v72, pp.129-133, 2001. [20] Heyries, Kevin A., Loughran, Michael G., Hoffmann, Daniel, Homsy, Alexandra, Blum, Loïc J., Marquette, Christophe A, “Microfluidic biochip for chemiluminescent detection of allergen-specific antibodies” , Biosensors and Bioelectronics, v 23, n 12, p 1812-1818, July 15, 2008. [21] Kim, Hae Jin; Ahn, Jeong Keun; Kim, Do Hyun, “Microfluidic chip with porous anodic alumina integrated with PDMS/glass substrate for immuno-diagnosis,” Current Applied Physics, v 9, n 2 SUPPL., p e60-e65, March 2009 [22] Thayne Edwards, Bruce K. Gale, A. Bruno Frazier, “Microscale Purification Systems for Biological Sample Preparation,” Biomedical Microdevices, v3:3, pp.211-218, 2001. [23] Bin-Wha Chang, Che-Hsiung Chen, Shin-Jyh Ding, David Chan-Hen Chen, Hsien-Chang Chang, “Impedimetric monitoring of cell attachment on interdigitated microelectrodes,” Sensors and Actuators B, v105, pp.159-163, 2005. [24] Junya Suehiro, Akio Ohtsubo, Tetsuji Hatano, Masanori Hara, “Selective detection of bacteria by a dielectrophoretic impedance measurement method using an antibody-immobilized electrode chip,” Sensors and Actuators B, v119, pp.319-326, 2006. [25] Mehdi Javanmard, AmirAli H. Talasaz, Mohsen Nemat-Gorgani, Fabian Pease, Mostafa Ronaghi, Ronald W. Davis, “Targeted cell detection based on microchannel gating,” Biomicrofluidics, v1, pp.044103, 2007. [26] H. J. Butt, “A sensitive method to measure change in the surface stress of solids,” J. Coll. Interf. Sci., v180, pp.251-260, 1996. [27] R. Raiteri, G. Nelles, H. -J. Butt, W. Knoll, P. Skladal, “Sensing of biological substances based on the bending of microfabricated cantilevers,” Sensors and Actuators B Chemical, v61, pp.213-217, 1999. [28] M. Alvarez, A. Calle, J. Tamayo, L. M. Lechuga, A. Abad, A. Montoya, “Development of nanomechanical biosensors for detection of the pesticide DDT,” Biosensors and Bioelectronics, v18, pp.649-653, 2003. [29] Y. Lee, G. Lim and W. Moon, “A self-excited micro cantilever biosensor actuated by PZT using the mass micro balancing technique,” Sensors and Actuators A Physical, v130, pp.105-110, 2006. [30] Siyang Zheng, Mandheerej S. Nandra, Chi-Yuan Shih, Wei Li, Yu-Chong Tai, “Resonance impedance sensing of human blood cells,” Sensors and Actuators A, v145-146, pp.29-36, 2008. [31] Y. H. Yun and A. Bange, A nanotube array immunosensor for direct electrochemical detection of antigen-antibody binding, sensors and actuators B , 123, 177-182,2007 [32] Haizhen Huang, Zhigang Liu, Xiurong Yang, Application of electrochemical impedance spectroscopy for monitoring allergen–antibody reactions using gold nanoparticle-based biomolecular immobilization method, Anal. Biochem. 356 208–214, 2006 [33] Haizhen Huang , Pixin Ran , Zhigang Liu, Impedance sensing of allergen–antibody interaction on glassy carbon electrode modified by gold electrodeposition, Bioelectrochemistry 70 257–262, 2007 [34] H. Wang, J.H. Kim, M. Zou, S. Tung, and J.W. Kim, “Adhesion Study of Escherichia coli Cells on Nano-/Microtextured Surfaces in a Microfluidic System,” IEEE Transactions on Nanotechnology, v7, pp.573-579, 2008. [35] P. Tabeling, “Introduction to Microfluidics,” Oxford University Press, New York, 2005 [36] M.R. King, “Cell-surface Adhesive Interactions in Microchannels and Microvessels,” Nanoscale and Microscale Thermophysical Engineering, v9, pp.255-264, 2005. [37] Washington State University: [38] Dennis Bray, “Cell Movement: from molecules to motility 2nd edition,” Garland Publishing, New York, 2001. [39] 高濂、孫靜、劉陽橋,「奈米粉體的分散及表面改性」,五南圖書出版股份有限公司,2005。 [40] 侯孟助,「新型病毒感測器之可行性分析」,碩士論文,國立中興大學機械工程研究所,2008。 [41] 曾秉國,「利用折疊型微流晶片提升蕪菁黃色嵌紋病毒(TYMV)於感測平面之接附密度與覆蓋均勻度」,碩士論文,國立中興大學機械工程研究所,2009。 [42] C.W. Hong, “From Long-range Interaction to Solid-body Contact Between Colloidal Surface During Forming,” Journal of the European Ceramic Society, v18, pp.2167-2169, 1998. [43] E. M. Purcell , “Life at low Reynolds number,” America Journal of Physics, v45, pp.3-11, 1977. [44] P. Tabeling, “Introduction to Microfluidics,” Oxford University Press, New York, 2005. [45] 黃心儀,「LEICA TCS SP5-共軛焦顯微鏡的原理及應用」,產品技術說明,美嘉儀器股份有限公司。 [46] Bard, Allen J.; Faulkner, Larry R., “Electrochemical Methods: Fundamentals and Applications,” JOHN WILEY & SONS, INC., New York, 2001 [47] Iseki S, Ohashi K, Nagaura S. Electrochim Acta, 1972, 17:2249. [48] 洪羚軒,「利用微環境影響黃色嵌紋病毒於感測表面之著附效果」,碩士論文,國立中興大學機械工程研究所,2010。 [49] 游文淮,「以離散元素法模擬膠粒粒間作用與布朗運動之研究」,碩士論文,國立台灣大學土木工程學研究所,2003。 [50] Michael W. Davidson - National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.
本研究成功利用3D奈米金顆粒感測器搭配微流裝置來偵測塵螨過敏原(Derp2)。首先利用陽極氧化製程製作陽極氧化鋁模(AAO),接著蝕刻陽極氧化鋁模,使得背阻障層呈半球形狀。利用電鑄技術,將Ni沉積上製成3D凹洞狀Ni模。接著使用熱壓技術製成3D半球型PC塑膠材料。接著在3D半球形PC上濺鍍一層金導電層,並利用電化學沉積法於奈米結構上均勻沉積大小約為10 nm之奈米金顆粒,利用此技術提升感測區域面積,結合微流環境形成高靈敏度奈米生醫檢測晶片

A 3D gold nano particle (NP) seneor in serpentine microfludic channel (S channel) was developed and has the advantage of label-free detection, high sensitivity and low cost. In the fabrication of 3D gold NP sensor, we demonstrated a replica mold fabrication method for nano hemisphere arrays by nanomolding nickel electroforming the barnier-layer surface of an anodic aluminum oxide (AAO) as the master mold. The fabricated 3D nickel mold is further used for replica molding of a nano-structure polycarbonate (PC) substrate by hot embossing. Afterwards, a layer of gold film and 3D NPs were deposited on the PC substrate by sputtering and electrochemical deposition, respectively. Compared to a flat gold film, 3D gold NPs have 3 times surface area which can highly increase the sensitivity of sensor. Furthermore, a serpentine microfludic channel was fabricated by soft lithography and polydimethylsiloxane (PDMS). Then, 3D gold NPs sensor were bonded on the S channel by O2 plasma treatment. D. pteronyssinus (Der p2) and specific antibody (C1) were used to present the performance of sensor. Electrochemical impedance spectroscopy (EIS) and fluorescence detection by confocal microscope were used to detect the concentration of Der p2.
In the EIS analysis, the minimum detectable concentrations of Der p2 were down to 0.1pg/ml and 10pg/ml when the 3D gold NPs sensor in S channel and in immerse deposition, respectively. In the fluorescence detection, the average coverages were 35.59% and 11.44% at the concentration of Der p2 10pg/ml, when sensor in S channel and in immerse deposition. Experiment at results showed that 3D gold NP sensor in S channel have 100 times sensitivity than sensor than sensor in immerse deposition. In the other hand, EIS analysis showed larger sensing linearity than fluorescence detection because fluorescence was saturated when the concentration of Der p2 was larger than 1ng/ml. Our results showed that 3D gold NPs sensor in S channel have great sensitivity (0.1pg/ml) compared to ELISA (1ng/ml).
其他識別: U0005-2208201115352200
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