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標題: 以中空纖維支撐液相微萃取結合液相層析-螢光偵測分析樣品中烷基苯酚
Determination of alkylphenols in environmental sample using hollow fiber protected LPME by HPLC-FLD
作者: 許宗洲
Shiu, Tzung-Jou
關鍵字: Alkylphenols;烷基苯酚;LPME;Hollow fiber;HPLC/FLD;液相微萃取;中空纖維管
出版社: 化學系所
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本實驗探討利用中空纖維支撐液相微萃取技術結合高效液相層析-螢光偵測系統分析環境樣品中烷基苯酚之可行性。實驗中探討液相微萃取之前處理條件(萃取溶劑、萃取時間、萃取攪拌速率、pH值影響、鹽類添加及層析條件),以尋求最佳條件來進行分析物之測定。根據實驗結果顯示以HF-LPME/FLD方法分析環境樣品中烷基苯酚之最佳條件為:萃取溶劑Bromooctane、萃取時間20分鐘、萃取攪拌速率400rpm、pH值調整為6.0、鹽類添加10%NaCl。以利用螢光偵測器(λex=222nm,λem =300nm)分析烷基苯酚類,其線性範圍位於1ng/mL~100ng/mL (butylphenol)和2ng/mL ~200ng/mL (octylphenol、nonylphenol),偵測極限為0.8ng/mL (butylphenol)、0.5ng/mL (octylphenol、nonylphenol)。且利用該方法來偵測環境中工業廢水及聚乳酸生物可分解材質(Polylactic acid, PLA)免洗塑膠杯之烷基苯酚可得到良好的再現性及回收率。

Alkylphenol is a kind of plasticizer and anti-oxidant of plastics and rubber and effective non-ionic surfactant utilized in domestic detergents and cleaners. The toxicity of alkylphenol is not very high, however its structure is similar to natural estrogen. It can result in the disruption of several processes by interfering with the signals that control the overall physiology of the organism. So researches have testified that alkylphenol is the environmental hormones. And alkylphenols easily exist in environment by biodegradation and bioaccumulation.
Because the conventional analytical method always need complicated pretreatment steps and larges quantity of organic solvent. In this study, hollow fiber protected liquid phase microextraction is applied as pretreatment step for the determination of alkylphenols in environment samples. This method not only used less organic solvent and simplified operation steps, but also decreased interferes from matrix.
In this study, we wanted to confirm the practicability of using HF-LPME/HPLC-FLD to determine alkylphenol in environment samples. In order to find the best conditions to determine analytes, we confirmed pretreatment conditions included extraction solvent, extraction time, stirring speed, effect of pH value and effect of salting out. Results indicated that the optimal sampling conditions as with bromooactane in hollow fiber to collect analytes of 25mL sample solution (with 10% sodium chloride and pH value was controlled to 6.0) at 400 rpm stirring for 20 mins, and then was determined by fluorescence detector. Under the optimal condition, the detection were in the concentration of 1~100 ng/mL for butylphenol, and is at 2~200 ng/mL for octylphenol and nonylphenol. The detection limited were 0.8 ng/mL for butylphenol, and 0.5 ng/mL for octylphenol and nonylphenol. Besides we obtained good reproducibility and recovery when using this method to determine analytes in the wastewater of the factory and the plastics cup of PLA.
其他識別: U0005-1507200718211300
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