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標題: 利用液相層析串聯質譜技術針對魚腥草中生物鹼之定量檢測
Determination of alkaloids in Houttuynia cordata by liquid chromatography/electrospray ionization-tandem mass spectrometry
作者: 周芳如
Chou, Fang-Ju
關鍵字: aristolactam;生物鹼;cepharadione;Houttuynia cordata;LC/ESI-MS/MS;魚腥草;液相層析串聯質譜
出版社: 化學系所
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This research is to evaluate the determination of aristolactam AⅡ, aristolactam BⅡ and cepharadione B as target markers in crude methanol extracts of Houttuynia cordata by using LC/ion trap tandem MS analysis.

By optimizing the chromatographic separation and mass spectrometric parameters, the precursor ions of the derivatives with the best responses were found, and the sensitivities in the determination of the three derivatives were improved. The optimal ionization behavior resulted when using electrospray ionization (ESI) in positive-ion mode with 0.4% acetic acid as an additive in the mobile phase. The selected reaction monitoring (SRM) mode provided additional improvement in selectivity. The analysis time including column equilibrium was 30 min per sample. The limits of detection (LODs) for aristolactam AⅡ, aristolactam BⅡ and cepharadione B were 20.8, 20.8, and 16.1 ng/g, respectively. The linearity range was 25-2000 ng/g.
其他識別: U0005-2707200911025400
Appears in Collections:化學系所

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