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標題: Nafion 修飾型紅外光感測器量測尿液中肌酸酐含量之效果探討
Development of Nafion-treated Infrared Chemical Sensing System for Detecting Creatinine in Urine Sample
作者: 余奕麟
Yu, Yi-Lin
關鍵字: Infrared;紅外光;ATR;Nafion;Creatinine;減弱式全反射;Nafion;肌酸酐
出版社: 化學系所
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本研究採用紅外光減弱式全反射(attenuated total reflection, ATR)結合固相微量萃取法,利用紅外光於紅外光感測晶體內發生全反射,但當紅外光於密介質到疏介質時,發生約微米長的漸逝波穿出晶體,此時晶體上修飾之特殊高分子與欲分析之待測物間產生特殊作用力使分子濃縮於漸逝波範圍內,產生分子的特性吸收光譜,以此來定性或定量欲分析物種。本實驗使用 ZnSe 作為 ATR 晶體,以 Nafion 作為修飾於晶體上的高分子層,利用 Nafion 的化學惰性、與具親水性和疏水性共存形成親水性通道和磺酸根官能基團與肌酸酐(creatinine)的特殊作用力,達成微量分析和選擇性提升的目的。為了解 Nafion修飾元件之感測性質以及最佳化感測條件,本研究針對可能影響訊號分析之因子如修飾層的穩定度、覆膜方式及厚度、酸鹼值、選擇性、干擾行為、線性濃度區間和訊號再現性,皆詳加討論。實驗結果顯示,Nafion 修飾層的於水溶液中的穩定性良好,於酸性條件下可有效的快速感測肌酸酐,以訊號比雜訊等於三為依據,計算所得之偵測極限約為 460 nM,而線性範圍可至 150 μM,在干擾行為方面,為解決尿液樣品中眾多干擾基質,故本研究利用稀釋樣品降低訊號干擾,再利用標準添加法可快速準確的定量出尿液檢體中對肌酸酐含量。

A simple and fast method based on evanescent-wave infrared
spectroscopy was proposed in this research for determination of creatinine in urines. Nafion was selected to modify the surface of the sensing element to increase both the sensitivity and selectivity in the detection. Nafion bears sulfonic acid group and tends to form water
channels after treated on the sensing element. The water channels allow the interested molecules to rapidly penetrate onto the Nafion thin film to concentrate the analytes for detections. The functional group of sulfonic
acid provides both hydrogen bonding and charge interactions to selectively interact with specific compounds, such as creatinine. To examine the sensing properties of Nafion and to optimize the conditions for detection, factors such as the pH of the sample solutions, the film thickness of Nafion, the long-term stability of sensing phase, regeneration efficiency, interferences of the sensing phase and the linear range in detection were explored. Results indicated that the detection of creatinine favored an acidic condition to increase charge-charge interactions. Under a detection time of 1 min, detection limits around 460 nM could be obtained with a linear range up to 150 μM. In the analysis of interferences, Nafion modified phase has no response to negative charged species owing to the charge repulsion between sulfonate and interferences. Strong interactions from positive charged species were observed.
To minimize the interferences in real-world samples, a dilution technique was proposed and examined. This proposed method allows Nafion modified surface sensor to determine the levels of creatinine in the realworld samples accurately.
其他識別: U0005-3107200905390600
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