Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/16810
標題: 利用線上微透析及分子印模固相萃取結合HPLC-UV分析巴克素之研究
Online microdialysis and molecular imprinted polymer solid-phase extraction coupled with HPLC-UV for the determination of paclobutrazol
作者: 林安邦
Lin, An-Bang
關鍵字: paclobutrazol;巴克素;microdialysis;MIP-SPE;HPLC;微透析;分子印模固相萃取;高效能液相層析
出版社: 化學系所
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摘要: 
巴克素(Paclobutrazol)為一種植物生長調節劑,可破壞植物內部激勃素的合成,抑制植物地上部的生長,在農藝、園藝等領域有許多的應用。為了瞭解農作物及土壤環境的殘留,本研究利用微透析及分子印模固相萃取以線上連接的方式結合HPLC-UV進行巴克素之分析。微透析作為第一次濃縮及隔離基質干擾的工具,而分子印模固相萃取則是以sol-gel process進行表面印模合成的slica gel來填充管柱,作為第二次濃縮及提高選擇性的場所。影響本技術的因素於研究中被詳細探討,以獲得最佳化分析操作條件,並用於實物之分析。
研究結果顯示,利用微透析及分子印模固相萃取線上連接HPLC-UV的技術可有效縮短前處理時間及提昇萃取的選擇性與效果。透過本方法可以快速、低成本地進行樣品檢測,有效降低基質干擾,並且省去過多繁複的前處理步驟,及避免使用有機溶劑於樣品前處理。本技術可望應用在生化、農藝、園藝、食品以及環境等方面樣品巴克素含量之檢測。

Paclobutrazol is a plant growth regulator used to inhibit the biosynthesis of gibberellin and reduce the terminal growth further. Nowadays, it widely applies in the field of agriculture and horticulture. In order to estimate the residue for paclobutrazol in soil and crop, we utilize microdialysis combined with molecular imprinted polymer online solid phase extraction as pretreatments, and HPLC-UV for the determination. Microdialysis is a tool to concentrate and exclude the substrate in sample. Molecular imprinted polymer modified silica gel synthesis processes surface imprinting via sol-gel process. The pre-column packed by molecular imprinted polymer modified silica gel has high selectivity and provides enrichment also. Factors which affect this experiment are well discussed to optimize the analysis conditions and be applied for real samples.
Online microdialysis and molecular imprinted polymer solid phase extraction coupled with HPLC-UV is a rapid, convenience and cost-less method for the determination of paclobutrazol. It can still reduce the interference of the substrate and provide high selectivity. This method is expected to be utilized to analyze the paclobutrazol residue in the field of agriculture, horticulture, biochemistry, food and environment in the future.
URI: http://hdl.handle.net/11455/16810
其他識別: U0005-2008201013475200
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