Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/16817
標題: 以超音波萃取結合非溶劑分離萃取技術及氣相層析電子捕捉偵測器分析柳丁果皮中農藥殘留量
Application of ultrasonic-assisted extraction couple non-solvent extraction for the determination of pesticides in orange samples with gas chromatography-micro electron capture detector
作者: 邱翊桓
Chiou, Yi-Huan
關鍵字: pesticide residues;有機磷;ultrasonic-assisted extraction;non-solvent extraction;除蟲菊;超音波萃取;非溶劑分離
出版社: 化學系所
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摘要: 
鑒於保護人民之健康安全及環境保育,全球漸趨向重視於農作物中農藥的使用量與殘留量,對於各類農作物中農藥殘留的分析方法開發並符合現行農藥最大容許濃度參考標準勢在必行。然而在分析方法開發設計時,往往忽略農藥在於樣品之使用方式,影響最後分析結果與準確度,因此在研究分析方法同時需考慮更加全面性,並針對性設計整體實驗流程與參考標準。在此研究中,開發一項快速、簡單及有機溶劑用量少之前處理技術,利用超音波萃取結合非溶劑分離萃取技術應用於柳丁果皮上兩種有機磷類(陶斯松、愛殺松)與四種除蟲菊類(賽洛寧、畢芬寧、芬普寧、總百滅寧)農藥殘留使用氣相層析儀搭配電子捕捉偵測器作分析,並建立柳丁果皮上兩種農藥殘留量之新參考標準,改善公告方法之農藥最大容許濃度以重量為單位轉變成以面積為單位。
考慮到會對萃取效率有所影響之條件進行最佳化探討,包含萃取溶劑之選擇搭配、萃取溶劑混合比例、非溶劑中鹽類濃度、非溶劑體積、非溶劑pH值、超音波萃取時間、非溶劑分離時間等探討。研究結果顯示,取柳丁果皮固定面積2.25cm2約0.9g置於10mL針筒中,加入1 mL 4%甲苯與丙酮混和萃取溶劑以超音波震盪3分鐘,取5mL非溶劑添加於針筒內並以轉速3200rpm離心4分鐘為最佳化條件確立,使用實驗室開發玻璃毛細取樣管裝置取樣,取1 microL萃取相注入GC-ECD分析。對樣品前處理時間約8分鐘即可完成。相關係數(R2)在0.9917~0.9992之間,偵測極限在0.04~1 ng/g之間。以單位面積上所殘留農藥量表示,相關係數(R2)在0.9929~0.9992之間,偵測極限在0.02~0.6 ng/cm2之間。應用於此方法可在短時間內完成多種農藥快速偵測,過程操作簡便快速,有機溶劑使用量少,並建立以單位面積上農藥殘留量為新參考標準。
URI: http://hdl.handle.net/11455/16817
其他識別: U0005-2308201011014700
Appears in Collections:化學系所

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