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標題: NNO-Schiff Base 之二價銅錯合物的合成與鑑定並探討其DNA光裂解的活性及細胞光毒性
Synthesis and Characterization of NNO-Schiff Base Cu(II) Complexes: Their Application in DNA Photocleavage and Photocytotoxicity
作者: 賴盈如
Lai, Ying-Ju
關鍵字: Schiff Base Cu(II) Complexes;Schiff Base 二價銅錯合物;DNA Photocleavage;Photocytotoxicity;DNA光裂解活性;細胞光毒性
出版社: 化學系所
引用: 1. 行政院衛生署 2. Wang, D.; Lippard, S. J. Nature Reviews Drug Discovery 2005, 4, 307-320. 3. Nishiyama, N.; Morimoto, Y.; Jang, W. -D.; Kataoka, K. Advanced Drug Delivery Reviews 2009, 61, 327–338. 4. Lerman, L. S. J. Mol. Biol. 1961, 3, 18. 5. Toshima, K.; Takano, R.; Ozaka, T.; Matsumura, S. Chem. Commun. 2002, 212 – 213. 6. Kathryn, E. E.; Duncan, T. O.; Barton, J. K. Chem. Rev. 1999, 99, 2777-2795. 7. Saha, S.; Majumdar, R.; Roy, M.; Dighe, R. R.; Chakravarty, A. R. Inorg. Chem. 2009, 48, 2652–2663. 8. Gewies, A. Aporeview, 2003, 1-26. 9. Sasmal, P. K.; Saha, S.; Majumdar, R.; Dighe, R. R.; Chakravarty, A. R. Inorg. Chem. 2010, 49, 849-859. 10. (a) Sigman, D. S. Acc. Chem. Res. 1986, 19, 180-186. (b) Barton, J. K. Science 1986, 233, 727-734. (c) Kalsbeck, W. A.; Grover, N.; Thorp, H. H. Angew. Chem. Int. Ed. Engl. 1991, 30, 1517-1518. (d) Erkkila, K. E.; Odom, D. T.; Barton J. K., Chem. Rev. 1999, 99, 2777-2795. (e) Zhou, Q. X.; Lei, W. H.; Sun, Y.; Chen, J. R.; Li, C.; Hou, Y. J.; Wang, X. S.; Zhang, B. W. Inorg. Chem. 2010, 49, 4729-4731. 11. (a) Watts, D. L. J. Orthomol. Med. 1989, 4, 165-169. (b) Marzano1, C.; Pellei, M.; Tisato, F.; Santini, C. Anti Can Age Med Chem, 2009, 9, 185-211. (c) Tan, S. J.; Yan, Y. K.; Peng, P.; Lee, F. Lim, K. H. Fut Med Chem, 2010, 2, 1591-1608. 12. Shanta, D; Chakravarty, A. R. Inorg. Chem. 2003, 42, 2483-2485. 13. Aguirre, J. D.; Angeles-Boza, A. M.; Chouai, A.; Pellois, J.; Turro, C.; Dunbar, K. R. J. Am. Chem. Soc. 2010, 131, 11353. 14. Saha, S.; Majumdar, R.; Roy, M.; Dighe, R. R.; Chakravarty, A. R. Inorg. Chem. 2009, 48, 2652.
合成一系列以NNO-Schiff Base (L1-L3)與聯吡啶共配位基的二價銅錯合物[Cu(L)B](ClO4) (B = bpy, a; phen, b; dpq, c; dppz, d; dppn, e)。錯合物[Cu(L)B](ClO4)結構經元素分析、ESI-MS及X-ray單晶繞射鑑定,並由CV、UV/Vis吸收光譜與EPR探討其物理性質。
在DNA光裂解實驗中,利用紫外光(365 nm)與藍光(430 nm)為燈源,探討錯合物之DNA光裂解活性與反應機制,並進一步探討錯合物在HeLa、SCC-15、L929癌細胞的光毒性。其中3e在藍光下具有較好的光動力潛力,期望在生物醫藥方面有更廣泛的應用。

A series of copper(II) complexes [Cu(Ln)B](ClO4)] (n=1 - 3,B= bpy, a;phen, b; dpq, c; dppz, d; dppn, e) have been synthesized and characterized by elemental analysis, X-ray crystal structural, LC/MS, Cyclic Voltammetry and UV-Vis spectroscopy.
Their activity and mechanism for DNA photo-induced cleavage are also investigated under UVA light (365 nm) and blue light (430 nm) condition. Furthermore, cellular experiments in HeLa, SCC-15 and L929 cancer cells indicate that complexes 1e, 2e and 3e show very high photo-induced cytotoxicity in which 3e has the best photodynamic therapy potential in blue light (430 nm). These results give impetus to new therapeutic strategies for developing photodynamic drugs.
其他識別: U0005-2606201222135900
Appears in Collections:化學系所

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