Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/1698
標題: 研究細胞運動性微流晶片之設計與製作
Design and fabrication of microfluidic chip for studying cell motility
作者: 戴瑋
Tai, Wei
關鍵字: microfluidics;微流道;metastasis;cell motility;cancer cell migration;cell sorting;轉移;細胞運動性;細胞遷移;細胞篩選
出版社: 機械工程學系所
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摘要: 
本論文將呈現一種研究細胞運動性的微流晶片。目前細胞運動性的研究包括wound healing assay和微流裝置都有些限制。例如wound healing assay可藉由觀察細胞兩個不同時間點得知細胞運動性,但卻無法精確的操作細胞,也無法避免群體細胞之間的相互影響。近年來文獻中提到的一些微流道的方式可以精確的操作細胞並觀察單一細胞的運動性,但仍然無法給予細胞相同的起始條件,以達到更精確、客觀的實驗分析;也無法將不同運動性的細胞篩選並且取出微流道,做不同運動性細胞的後續分析和研究。
本研究為細胞運動性研究設計了一種新的微流晶片。首先,利用migration area與draining channel之間的液壓差將細胞排列成一直線,為細胞創造出相同的起始條件。第二,微流晶片中的每一顆細胞都被觀察和分析。第三,利用collection channels創造出的層流將微流晶片中的細胞按照其位置不同分別被收集出微流晶片。第四,創造濃度梯度場達成細胞趨化研究的功能。本研究提供了一個單一細胞層級的細胞運動性分析平台。
本研究使用人類的乳癌細胞MDA-MB-231和MCF-7作為檢體,測試細胞於本微流晶片中的運動情形。我們成功的將細胞排列成一直線,為細胞創造出相同起始條件;我們精確的量化分析了微流晶片中單一細胞等級的細胞自發性移動,分析了細胞的速度、速率等等運動性的資料;我們將細胞依照其在微流晶片中不同的位置分別收集到不同的培養皿中,並且量化細胞回收率;我們創造出濃度梯度場,達到細胞趨化研究的功能。本研究提供了一個單一細胞層級的細胞運動性分析平台。本論文呈現了微流晶片的細胞排列、細胞運動性分析、細胞收集和創造濃度梯度場等功能。

In this thesis, we present a microfluidic chip for studying cell motility. There are some limitations for current cell motility research including wound healing assay and microfluidic device. For example, wound healing assay could observe two different time point of cultured cells. However, it is not able to manipulate cells precisely and avoid interaction between groups of cells in would healing assay. Recently, some reports in the literature could handle many single cells precisely in their microfludic devices. However, those devices still were not able to provide same initial conditions for cells to study motility. Also, those devices could not sort and collect cells based on their motility for the follow-up research.
In this research, a novel microfluidic device was developed to study cell motility. Firstly, cells in the microfluidic chip were able to align in a single line to create same initial condition for cell motility by different hydraulic pressure between migration area and draining channel. Secondly, motility of each cell was observed and analyzed. Thirdly, cells were collected based on their different position in this device by laminar flow created by collection channels. Fourthly, concentration gradient was formed for chemotaxis research. A new platform for cell motility analysis in single cell level was developed.
In this research, human breast cancer cells MDA-MB-231 and MCF-7 are used as samples to test motion of cells. Same initial condition for cells by align cells in one single line was achieved. Single-cell-level spontaneous migration was analyzed and quantified precisely. Velocities and rates of cell motility data were measured and analyzed. Cells were collected to different culture dishes based on their different position in microfluidic chip. Cell recovery rate was quantified. Concentration gradient was formed for chemotaxis research. A platform for cell motility research in single cell level was performed. The functions of microfluidic chip for cell alignment, cell motility analysis, cell collection and gradient formation were demonstrated.
URI: http://hdl.handle.net/11455/1698
其他識別: U0005-2505201210430500
Appears in Collections:機械工程學系所

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