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標題: 組織工程人工微血管之製作與細胞培養
Tissue Engineering Artificial Capillaries Fabrication and Cell Culture
作者: 徐一峯
Hsu, Yi-Feng
關鍵字: Tissue Engineering;組織工程;Cell Culture;Bio-MEMS;Artificial Capillaries;細胞培養;生醫微機電;人工微血管
出版社: 機械工程學系

Lacking the inherent blood circulatory system to provide sufficient exchange of nutrient and oxygen is the most rigorous challenge of the artificial tissue and organ industry. One of the feasible solutions is to make an artificial microvessel system to enable the regular operation of metabolism. The main goal of this research is to integrate the micro electromechanical fabrication technique and the tissue engineering technique such that the artificial microvessel system can be realized.
At the first stage of this research, the commercial software FEMLAB was employed to optimize the network of the microchannels which were used as the scaffold to cultivate the endothelial cell. It was found that more than two vertical nodes in the network caused unbalanced hydrodynamics among the microchannels. The design principle came out to be a network made up of multi branches with no more than two vertical nodes in individual branch.
Following, a simple and inexpensive lithograph approach, in which the PMMA polymer was selected to be the substrate, the negative photoresist JSR was employed to form the microchannel structure, was adopted to fabricate the microvessel scaffold. In addition, a soft PDMS based microvessel scaffold was built by using a mold that was made up of the negative photoresist JSR. With O2 plasma treatment, the PDMS based microvessel scaffold became more hydrophilic such that the cell culture could be easier to conduct.
During cell culture, it was found that the PMMA base scaffold enabled the bovine endothelial cells (BEC) to statically grow. However, the overall exchange of nutrient and oxygen was inefficient. The scaffold made up of the soft PMMA and processed by O2 plasma could achieve better results. Dynamic seeding was further conducted to have better circulation of culture medium. The bovine endothelial cells could successfully be cultivated in the microvessel scaffold by dynamic seeding.
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