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標題: The Analysis of SELEX by statistical models: Aptamers are selected at pre-equilibrium state
作者: 陳淑姿
Tzu, Chen Shu
關鍵字: SELEX;aptamer;ligand;affinity;binding rate;dissociation rate.
出版社: 應用數學系
We develop a new mathematical model and computer simulations for SELEX (Systematical Evolution of Ligands by EXponential enrichment) experiments. By considering binding and dissociation rates of ligands and targets, we investigate quantitatively the effect of incubation time of binding reactions on the enrichment of high affinity ligands. We incorporate partition efficiency, background contamination and initial winner frequency in our simulations. In most cases, we find that with insufficient incubation times SELEX can still generate aptamers for targets, yet the full enrichment of the ligand library is at the expense of selection cycles.
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