Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/19774
標題: 預測轉位因子關聯之微核醣核酸與其可能調控目標之分析
Prediction of the transposable element derived microRNAs and analysis of their regulatory targets
作者: 劉倍瑜
Liou, Bei-Yu
關鍵字: micro RNA;微核醣核酸(miRNA);transposable element;target prediction;轉位因子(transposable element, TE);目標基因預測(target prediction)
出版社: 資訊網路多媒體研究所
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摘要: 
微核醣核酸(microRNA,miRNA)為髮夾狀的小片段非編碼核糖核酸(noncoding small RNA),普遍存在於動物、植物及病毒的基因體中,主要參與調控胚胎發育、細胞分化、細胞死亡、染色體結構、抗病毒反應、致死基因生成等的基因表現。根據近年來已知的研究報告中證實微核醣核酸與轉位因子(transposable element,TE)間有著密切的關聯性,且經生物實驗研究發現到有些特定的轉位因子序列經過演化後,可衍生出類似微核醣核酸的莖環結構。依過去的研究指出人類基因體中有超過50%是由重複性序列所組成,且這些重複性序列中超過45%是由轉位因子衍生而來。TE,又可稱為移動因子(mobile element),其轉位(transposition)作用會導致基因組的結構發生改變,並因而影響到轉位發生處基因的表現。因此本研究的目的主要在分析探討序列中哪些微核醣核酸同時屬於轉位因子,亦即所謂的TE-derived miRNAs。接著再將預測出的成熟的微核醣核酸序列(mature miRNAs)結合(target)到特定信使核醣核酸(mRNA)的3''端未轉譯區(3''-UTR),藉以觀察了解TE基因可能的分布趨勢與其調控目標。

Micro RNAs(miRNAs) are a hairpin-type small RNA. They exist in animals'', plants'' and virus'' genome. miRNA regulation of gene expression is involved in embryonic development, cell proliferation, cell differentiation, cell dying, chromosome structure, and antiviral response. According to previous studies, human genome contains more than 50% of repetitive sequences, and more than 45% of these repetitive sequences are derived from transposable elements. The transposition of the transposable element, TE, which is also called the mobile element will lead to mutation in the genome structure and affect the translocation at the gene expression. The purpose of this study is major in the analysis of miRNA gene sequences, which may be derived from the transposable elements. This kind of miRNAs is called TE-derived miRNAs. We also target the sequences of the TE-derived miRNAs to the specific messenger RNAs in order to observe the distribution of transposable elements.
URI: http://hdl.handle.net/11455/19774
其他識別: U0005-0808201111393700
Appears in Collections:資訊網路與多媒體研究所

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