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標題: PTPγ在台灣女性乳癌的高度表現
Overexpression of protein tyrosine phosphatase gamma in Taiwanese women with breast cancers
作者: 林如璠
Lin, Ju-Fan
關鍵字: breast cancer;丙型蛋白質酪胺酸磷酸酶;PTPgamma
出版社: 生物醫學研究所
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丙型蛋白質酪胺酸磷酸酶 (protein tyrosine phosphatase gamma, PTPγ)位於第三對染色體短臂14.2的位置,其被推測可能為一腫瘤抑制基因,但卻在淋巴癌及星狀細胞瘤有高度表現的情形。在本研究中,藉由RT-PCR、原位雜交 (in situ hybridization, ISH)及西方墨漬法 (western blot)等技術,我們發現PTPγ在乳癌細胞株及台灣女性乳癌組織檢體都有高度表現的情形。此外,我們也沒有在細胞株及臨床檢體中發現任何特殊的ptprg基因缺失 (deletion)及突變 (mutation)。免疫組織染色結果顯示PTPγ主要分布於腫瘤組織,且令人意外地,有約30%的病患其PTPγ有進到細胞核內的情形。進一步地,我們發現幾乎所有的浸潤性腺管癌 (infiltrating ductal carcinoma, IDC) 的病患,雖其細胞分化程度較差,但都有PTPγ的高度表現;而這樣的情形卻只在58%的浸潤性小葉癌 (infiltrating lobular carcinoma, ILC)病患見到。綜合上述結果,我們證實了PTPγ在台灣女性乳癌的確有高度表現,其可能扮演致癌基因的角色,並可能為一乳癌預後因子及潛在的治療標的。

Breast cancer is a leading cause of cancers in Taiwanese women. The incidence and mortality rate increased rapidly in the past two decades. The average onset age of breast cancer in Taiwan is 8-10 years younger than that in Euro-America. However, the molecular difference is still unclear.
Protein tyrosine phosphatase gamma (PTPγ) is located at chromosome 3p14.2. This molecule is suggested as a tumor suppressor candidate, but is up-regulated during lymphoma and astrocytoma tumorigenesis. In this study, we found that PTPγ was overexpressed in breast cancer cell lines and breast cancer specimens by using RT-PCR, in situ hybridization, and western blot analysis. However, no specific deletion or mutation was found in PTPγ of cancer cell lines or clinical samples. Immunohistochemical staining showed that PTPγ was mainly distributed in tumor lesion, and surprisingly, PTPγ was located in the nucleus in about 30% of tumor samples. Moreover, we found that nearly all infiltrating ductal carcinoma (IDC), but only 58% infiltrating lobular carcinoma (ILC) patients had high level of PTPγ expression. These data indicated that PTPγ is a potential oncogene and may be a prognostic factor and therapeutic target of breast cancer in Taiwan.
其他識別: U0005-1108200810191500
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