Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/2018
標題: 製作表面奈米結構之生物可降解人工微血管支架
Fabrication of Biodegradable Microvessel Scaffold with Nano Patterned inner Wall
作者: 林晏成
Lin, Yen-Cheng
關鍵字: nano;奈米;biodegradable;scaffold;生物可降解;支架
出版社: 機械工程學系所
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摘要: 
PLGA為廣泛應用之生物可降解材料之一,而具奈米結構之PLGA更是在組織工程上有極大之應用潛能。本研究利用陽極氧化鋁模(AAO)結合真空抽氣壓印法之物理方式翻模,製作不同奈米結構之PLGA支架,以牛頸動脈內皮細胞之生長比較不同奈米結構對於細胞生長與貼附的影響。將PLGA溶液澆鑄於陽極氧化鋁模(AAO)上,經過1-2小時使得表面凝固;再放入真空烘箱經由抽氣使得PLGA半凝固態之PLGA材料能擠壓進入奈米孔洞中,形成柱狀奈米結構,奈米結構之尺寸可由抽氣時間加以控制;接著,將抽氣後的PLGA試片浸泡於磷酸溶液中去除氧化鋁,或直接脫膜,即可得到奈米結構PLGA支架,細胞貼附測試結果,可發現奈米結構PLGA支架有較好之細胞貼附性。本研究所提出之新方法除製程簡單外,更可藉由陽極氧化鋁模之製作控制奈米結構之圖案,使得奈米結構對細胞生長之影響之觀測更易於進行。因此本研究將人工微血管支架與奈米結構結合,以黃光微影技術製作JSR光阻母模,配合光阻熱熔法形成半圓形母模結構,再濺鍍金屬鋁薄膜於母模表面,經過陽極處理於表面生成奈米孔洞,再以一次翻模方式製作以PLGA為基材之半圓形微管道結構,最後以ICP接合形成具圓形截面之PLGA微血管道支架。此製程步驟簡單,並可製作出良好精度之微管道結構。在細胞培養方面,因PLGA膨潤無法觀測,於是本研究使用螢光染色方法觀測管道內細胞,PLGA具良好之生物相容性且由類動態培養有良好之培養機制,因此細胞於PLGA微管道內能有很好之貼附生長情形。
URI: http://hdl.handle.net/11455/2018
其他識別: U0005-1107200811181500
Appears in Collections:機械工程學系所

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