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標題: 立枯絲核菌第四融合群致病性與DNA變異之探討
Exploration of pathogenicity and DNA variation of Rhizoctonia solani AG-4
作者: 黃柏挺
Huang, Po-Ting
關鍵字: Rhizoctonia solani;立枯絲核菌;random amplified polymorphic DNA (RAPD);rDNA;riboprinting;pathogenicity;致病性;逢機增幅多型性核酸;核糖指紋
出版社: 植物學系
立枯絲核菌 (Rhizoctonia solani) 為一世界性分佈之土媒植物病原真菌
異。本論文為探討此菌之致病性與DNA 變異,選取15株台灣地區之立枯絲
核菌第四融合群 (R. solani AG-4) 菌株,及一HGI 標準菌株,共16株供
性菌株。篩選出12條逢機增幅多型性核酸 (RAPD) 之引子,增幅之產物經
洋菜凝膠電泳分析,記錄成二元 (binary) 資料,共獲得467個數據列,
(universal) 核糖核酸引子ITS1與ITS4 進行PCR,增幅之ITS1-5.8S
rDNA-ITS2之片段大小約 650至750 bp,再分別以內切限制EcoR I、Hae
III、Mbo I等切割,產生多種核糖指紋 (riboprinting) 圖譜。ITS1-5.8
S rDNA-ITS2片段大小與核糖指紋圖譜與供試菌株之致病性具有相關性。

Rhizoctonia solani K(hn is a world-wide soil-borne pathogenic
fungus which show tremendous variation in characteristics such
as geographic locations, morphology, host specificity and
pathogenicity. The relation of pathogenicity and DNA variation
of fifteen isolates of Rhizoctonia solani belonging to
anastomosis group (AG) 4 from Taiwan and one isolate belonging
to HGI were examined. The virulence of each strain was tested in
loose leaf lettuce seedlings. The results indicated that eight
strains were designated hypervirulent, two strains were middle
virulent, four strains were hypovirulent and two strains were
non-virulent of the sixteen tested strains. The genetic
variation of these strains were evaluated by random amplified
polymorphic DNA (RAPD) method with a set of twelve primers. The
amplification results revealed scorable polymorphisms among
these isolates, and a total of 467 band positions were scored
with binary coding and assayed for Numerical Taxonomy and
Multivariate Analysis System (NTSYS) software. The results of
analysis indicated that the DNA banding patterns of the highly
virulent isolates were highly similarity each other. Approximate
650 to 750 base pairs of ITS1-5.8S rDNA ITS2 fragments were
amplified by universal primers ITS1 and ITS4 of fungal ribosomal
DNA. The fragments were subjected to restriction digestion with
recognition endonucleases EcoRI, HaeIII, MboI and different
riboprinting patterns were displayed. The virulence of the
sixteen isolates can be related with differences in the size of
the fragments of ITS1-5.8S rDNA ITS2 and riboprinting patterns.
Appears in Collections:生命科學系所

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