Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/20913
標題: 臺灣地區孟宗竹族群核酸多型性遺傳變異之研究
Population genetic variation of Phyllostachys pubescens in Taiwan based on DNA polymorphisms
作者: 賴杰治
Lai, Chieh-Chih
關鍵字: Phyllostachys pubescens;孟宗竹;DNA polymorphism;genetic variation;核酸多型性;遺傳變異
出版社: 植物學系
摘要: 
孟宗竹乃屬於地下莖橫走側出單稈散生型竹類, 在台灣是重要經濟
竹類。 本研究的目地是以 RAPD 及微衛星與迷你衛星序列為引子放大基
因組DNA 的分析方法, 來探究孟宗竹的營養系(基因型)以及不同營養
系在台灣的分佈情況。從本省各地共採得 178 個孟宗竹、 4 個龜甲竹及
5 個江氏孟宗竹竹葉樣本進行分析。自每一個樣本所萃取的基因組 DNA
,以 13個 RAPD 引子、 3 個微衛星與 1 個迷你衛星引子進行 PCR 反應
,擴大基因組的 DNA 片段, 以此結果來鑑別營養系。研究結果共得 9
個孟宗竹營養系,以及 7 個龜甲竹與江氏孟宗竹營養系。 孟宗竹的
繁殖主要是依賴地下莖, 因此一般咸認為其遺傳變異必然有限。從本研
究之結果得以驗證此一觀點, 孟宗竹之遺傳變異主要集中於所採集的樣
區內, 地區間及樣區間分化情形並不顯著。對於本研究所分析出的基因
型, 可能是當初自大陸引進之時便已存在,而一直繁衍至今。然而由營
養系經體細胞的變異而衍生新系的可能性, 亦不能將之排除。研究結果
並發現所有的基因型在南投地區均有存在, 而其他地區幾乎均為同一營
養系。 此一事實或許可推想當初引進之時,南投可能為最早的栽植地,
其他地區是由此進一步引種栽植的。

Phyllostachys pubescens is a bamboo species with
leptomorphrhizomes. It is an economically important bamboo in
Taiwan. Theamplifying genomic DNA segments using RAPD,
microsatellite, andminisatellite sequences as primers and to
investigate the purposeof the present study is to identify
clones (genotypes) bydistribution of clones in Taiwan. One
hundred and seventy-eightsamples of the species, together with
5 samples of Phyllostachyspubescens cv."Tao Kiang" and 4
samples of Phyllostachys pubescensvar. heterocycla were
collected around the island. Genomic DNAfragment were
amplified using 13 RAPD primers, 3 microsatellitepatterns were
used for clone identification. Nine clones wereidentified
form 178 samples of the species. Seven clones wereidentified
from samples of cultivar and the variety. Phyllostachys
pubescens is reproduced mainly by rhizome. Itsgenetic variation
is generally expected to be limited. The mainsource of
variation is resided within sampling sites. Thereis no
significant genetic diversification between sites andbetween
regions. The observed clones in the present study mightbe
introduced from mainland china couple hundred years ago
andmaintained to the present day. However, we can not rule out
thepossibility that some of the clones might be originated
throughtsomatic mutation. The results also showed that the
regions aroundNan-Tou County consisted of all of the 9 clones of
the specieswhile the rest regions generally consisted of only 1
commonclone. This evidence indicated that Nan-Tou region might
be thefirst location of cultivation and later the cultivation
spreadedto other regions.
URI: http://hdl.handle.net/11455/20913
Appears in Collections:生命科學系所

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