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The physiological characterizatiions and cross protection of transformant from Rhizoctonia solani Kuehn
|關鍵字:||Rhizoctonia solani;立枯絲核菌;physiological;transformant;cross protection;生理特性;轉形株;交叉保護||出版社:||植物學系||摘要:||
立枯絲核菌(Rhizoctonia solani Kn)為本省重要的植物病原真菌，能
感染的寄主種類廣泛。R. solani第四融合群(anastomosis group 4,簡稱
之弱致病株則不具有線形質體；而R. solani CHU344+pUCH1-ES是經轉形
In Taiwan, Rhizoctonia solani Kuehnn is an important plant
pathogenic fungus. It possesses a wide host range. A strong
pathogenic strain CHU341 which belongs to anastomosis group 4
(AG-4) carries a linear plasmid about the size of 2.6 Kb.
Another strain CHU344 is a weak pathology without linear
plasmid. The other stable transformant CHU344+pUCH1-ES was
obtained from CHU341 and CHU344. This study analyzed the
relationship between R. solai linear plasmid and growth
conditions as well as their pathogenecity.The strong pathogenic
strain CHU341 is well adapted to carbon source and nitrogen
source. The weakly pathogenic strain CHU344 and the transformant
CHU344+pUCH1-ES are sensitive of carbon and nitrogen sources.
The optimal temperatures and pH-values for the growth of R.
solani strains are 27℃ and pH 6-7. The physiological
characteristics of transfor-mant are similar to that of the
weakly pathogenic strain CHU344. The results of the growth
condition tests could be used for the biological control and
selection of R. solani strains. The pathogenecity for radish of
the transformant was between CHU341 and CHU344, and the radish's
chitinase induced by the transformant had similar relation. The
above results suggested that the linear plasmid of R. solani may
consist a relationship with pathogenecity and the linear plasmid
as well as induction of chitinase.
|Appears in Collections:||生命科學系所|
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