Please use this identifier to cite or link to this item:
標題: 以RFLP分析玉米第八對染色體之尖端缺失
Characterization of terminal deficiency associated with the long arm of chromosome 8 in maize
作者: 張士鼎
Chang, Shih-Ting
關鍵字: maize;玉米;r-X1 deletion;monosome;partial monosome;RFLP;r-X1 缺失;染色體不分離;單染體;部分單染體;限制片段多形性;實體定位
出版社: 植物學系
(complete monosomy,簡稱CM)及部分單染體(partial monosomy,簡稱
的目的是利用已知位置之限制片段多形性(restriction fragment
length polymorphism,簡稱RFLP)標誌(markers)標定第8條染色體PM的斷
位於 RFLP標誌umc103A與umc12(umc93)之間,三株位於RFLP標誌umc12(
umc93)與 umc30之間,最遠有一株斷於umc30與j1基因座(locus)之間。利

The r-X1 deficiency in maize was first isolated with X
irradiation by L.J.Stadler. It is a submicroscopic, intercalary
deficiency located on chromosome 10. It is transmitted to
progeny only through the maternal plant. It can induce the
formation of monosomic (or CM) and partial monosomic (or PM)
progeny as the result of chromosome nondisjunction and breakage
that occur during megagametogenesis. The purpose of this study
is to map the breakpoints of paitial monosome 8s by using the
well-mapped RFLP (restriction fragment length polymorphism)
markers, and to use them to reevaluate the accuracy of current
RFLP linkage map of the long arm chromosome 8. In this study,
three partial monosomic plants were obtained, one of them had
been lost. The remaining two in addition to seven PMs obtained
earlier were used for RFLP analysis. The result shows that the
breakpoints of these nine PMs are not located at the same
chromosome location. Five of them are located in the 103A -
umc12 (umc93) region, and three others are located in the umc12
(umc93) - umc30 rejion. The last one is located in the region
between umc30 and j1 locus. The physical map of RFLP markers on
the long arm of chromosome 8 as constructed by using these nine
partial monosomes is consistent with the conventional linkage
map: no rearranged gene order is observed. This study also map
centromere of chromosome 8 to the chromosome region close to
umc 103A.
Appears in Collections:生命科學系所

Show full item record
TAIR Related Article

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.