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標題: 應用ISSR研究唐杜鵑之族群遺傳變異
Genetic Variation of Rhododendron simsii Based on ISSR Markers
作者: 陳硯茹
Chen, Yen-Ju
關鍵字: Rhododendron simsii;唐杜鵑;genetic variation;ISSR;Ericaceae;遺傳變異;ISSR;杜鵑花科
出版社: 生命科學系
杜鵑花屬是杜鵑花科的一個大屬。根據台灣植物誌第二版,台灣原生杜鵑屬植物有15種,其中12種為特有種植物。唐杜鵑(Rhododendron simsii Planch.)在台灣呈現特殊的南北分佈,個體變異極大,分類問題一直存有許多爭議。在形態研究部分,並無有力資訊可將唐杜鵑區分為南北兩群。本研究在ISSR研究部分,以9個ISSR引子檢視唐杜鵑台灣南、北及金門三個地區之8個樣點82個個體,共得到125個條帶,其中多型性條帶有109個(87.20%),單型性條帶有16個(12.80%)。AMOVA分析結果顯示,唐杜鵑3個地區8個樣點之樣點內個體間變方成分百分比為46.82%(P<0.001),較地區間的35.44%(P<0.001)為高,且兩者都遠大於地區內樣點間的17.74%(P<0.001)。POPGENE分析結果顯示,總Nei’s基因歧異度值為0.3423,遺傳分化指數(Gst)為0.5081,基因流(Nm)為0.4841。歸群分析與主座標分析結果顯示,唐杜鵑在地區間及樣點間遺傳分化顯著,首先可分為台灣南部、北部及金門三大群,南部樣點又可再分為南仁山及欖仁溪與壽卡兩小群,南仁山與欖仁溪之間亦有較小程度的分化,在北部樣點間則無顯著遺傳分化,這可能與其特殊的地理分佈情況有關。歸群分析與主座標分析結果與地理分佈位置吻合,相近地區間遺傳變異較小,顯示唐杜鵑的族群變異受到地理距離的影響。

Rhododendron is a large genus in the family Ericaceae. According to the second edition of the Flora of Taiwan, there are 15 species in Taiwan and among them 12 species are endemic. Rhododendron simsii Planch. is distributed discontinuously in southern and northern Taiwan. There are many different classification treatments of the species due to high degree of variation within this species. Morphological study indicated that there is no significant morphological difference between southern and northern populations. In the present study based on ISSR molecular markers, 82 individual samples at 8 sampling sites in 3 regions of southern Taiwan, northern Taiwan, and Kinmen were studied using 9 ISSR primers and resulted in a total of 125 bands. Among them, 109 bands were polymorphic ( 87.20 % ) while 16 band were monomorphic ( 12.80 % ). Analysis of molecular variance (AMOVA) revealed that variance components attributable to the difference among regions, the difference among sampling sites within regions, and the difference among individuals within sampling sites were 35.44% ( P < 0.001 ), 17.74% ( P < 0.001 ), and 46.82% ( P < 0.001 ), respectively. The result using POPGENE software indicated the total Nei's gene diversity index was 0.3423 and genetic differentiation index (Gst) was 0.581 while gene flow (Nm) was 0.4841. Cluster analysis and principal coordinate analysis revealed that the species is diverged genetically into southern Taiwan, northern Taiwan, and Kinmen groups. Within southern Taiwan group, it is further divided into Soka and Nanzunshan-Nunzunshi sub groups. A smaller but significant genetic differentiation was also observed between Nanzunshan and Nunzunshi sampling sites. However, there is no significant genetic differentiation among sampling sites within northern Taiwan region. The results of the present study indicated that the genetic differentiation of the species is correlated with geographic distance.
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