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標題: 應用ISSR研究刺柏之族群遺傳變異
Genetic Variation of Juniperus formosana Hayata Based on ISSR Markers
作者: 蔡依錡
Tsai, Yi-Chi
關鍵字: Juniperus formosana;刺柏;ISSR;genetic variation;ISSR;遺傳變異
出版社: 生命科學系
刺柏(Juniperus formosana Hayata)為台灣原生柏屬植物之一,

Juniperus formosana is generally distributed in mountain grasslands and rocky habitats above 2,500m in altitude in Taiwan. Few populations also occur in lower altitude on the east side of the island. It is a representative first or second successive tree species of the high mountain plant community. In the present study, 117 individuals from 8 populations were studied for ISSR variation. One hundred and twenty-six bands were obtained using 10 primers. Eighty bands (63.49%) were polymorphic bands. AMOVA analysis revealed that the perceontages of variance components attributable to variation among regions, among population within regions, and among individuals within populations were 5.26% (P<0.001), 18.96% (P<0.001), and 75.78% (P<0.001), respectively. POPGENE analysis revealed that the total gene diversity (Ht) of the species was 0.3536 and the gene diversity of populations ranged from 0.1593 to 0.2458. The population genetic diversity index (Gst) was 0.2177 while the gene flow index (Nm) was 1.797. The result indicated that the population differentiation was higher than the average of conifer species. There is a significant genetic difference between Liu-Shue population of Hualien County and the rest population. Sian-Yang population is significantly different from Yushan and Guanshan population of the same southern region. Shieshan population is significantly different from Dabagienshan population of the same moutain range.
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