Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/21779
標題: Functional Characterization of NGFB on the Malignancy of Lung Cancer
神經生長因子在肺癌惡性度之功能分析
作者: Su, Te-Jen
舒德仁
關鍵字: 非小細胞肺癌;NSCLC;神經生長因子;NGF
出版社: 分子生物學研究所
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摘要: 
Lung cancer has increased the most in mortality among all cancers worldwide in recent twenty years. In Taiwan, the average death of lung cancer every year is catching up those of liver cancer, and lung cancer takes the first place of death in female population and keeps on aggravating. Metastasis is the leading cause of death among patients with cancers and has been studied extensively for years. Many genes or proteins involved in invasion and metastasis have been identified and characterized in past decades; however, most of the molecules don't exist in metastatic cancer cells exclusively, in most cases, they also exist in normal cells. Microarray is a powerful tool to analyze gene expressions parallel and in large quantity, and has been utilized in search the differences of gene expressions as well as classification of diseases.
In earlier studies, studying a series of lung adenocarcinoma cell lines with varying degrees of invasiveness (invasiveness: CL 1-0In this thesis, we stress on exploring the possible role of NGF in tumorigenesis and metastasis further. We measured NGF mRNA expression by real time PCR in 77 non-small cell lung cancer surgical specimens, and correlated with the patient's clinical outcome. The result showed that the expression of NGF positively
correlated with survival of lung cancer patients (p=0.039). We investigated the effect of NGF on the invasion and migration of the lung adenocarcinoma cell line (CL1-0) with 2-3 fold of invasive capacity, which revealed that the increasing of NGF promoted tumor cell migration and invasion. To further elucidate the potential mechanisms by which NGF contributes to tumor invasion and tumorigenesis, Affymetrix genechips were used to profile the alterations of gene expression after overexpressing NGF. The microarray data demonstrated that NGF regulated genes involving in angiogenesis, cell proliferation, signal transduction cell migration and metastasis. Our studies herein provided a new insight into how NGF may contribute to tumor invasion and tumorigenesis.

近二十年來,肺癌是全世界癌症死亡率增加最快的疾病。在台灣,肺癌的平均死亡人數也直逼肝癌,在女性人口則為第一位,而且死亡率持續竄升。癌細胞轉移(metastasis)是癌症病患的主要死因,已被廣泛的研究多年。在過去幾十年,有許多參與腫瘤細胞侵入及轉移作用的基因或蛋白質被驗証及定性。然而,這些分子並非獨一且專一的存在於癌轉移細胞中,在大部分的例子,也存在於正常細胞中。而微陣列(microarray)為近年發展可大量平行分析基因表現的有力工具,亦已應用於許多差異表現基因的搜尋及臨床疾病的分類研究上。先前實驗中,利用cDNA微陣列研究具不同侵入能力(invasion)的肺癌模式細胞株(侵入能力:CL1-0本研究在探討NGFB在肺癌生成及轉移過程中可能扮演的角色,首先藉由即時定量RT-PCR(Real-time RT-PCR)分析77個非小細胞肺癌組織檢體中NGF表現量與其臨床診斷結果的相關性。結果發現,NGFB表現低的病人有較高的存活率(p值為0.039)。對於侵襲能力低的CL1-0細胞中,大量表現NGF,結果發現會促使細胞增加侵襲及轉移能力。
實驗結果顯示,NGFB可能與侵襲能力( cell invasion)、細胞遷移(cell migration)及轉移相關之基因調控有關,依照研究結果推測,增加NGFB的表現可
增加癌細胞侵襲能力及腫瘤生成的作用。
URI: http://hdl.handle.net/11455/21779
其他識別: U0005-2808200613273800
Appears in Collections:分子生物學研究所

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