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Studies of immunohistochemical staining on the distribution of antigen presenting cells (APC) in human intestinal tissues
Chen, Chiung Ju
|關鍵字:||antigen presenting cell;抗原呈現細胞;immunohistochemical stain;dendritic cell;macrophage;免疫組織化學染色法;樹突細胞;巨噬細胞||出版社:||生命科學院碩士在職專班||摘要:||
抗原呈現細胞具MHC class I / II分子且能呈現T細胞專一的抗原性胜肽給專一性T細胞，調控免疫反應。它具強烈免疫活化能力，能捕獲、處理抗原並呈現給輔助性T細胞或細胞毒殺性T細胞，調控專一性免疫反應。包含巨噬細胞、樹突細胞與B細胞三大類。
Antigen-presenting cells containing MHC class I & II molecules can process and present T cell peptide epitope to specific T cells, and thus regulate acquired immunity. They have powerful capability to capture, process and present antigens to specific Th cells or Tc cells. They play a major role in specific immune responses. They include macrophages, dendritic cells and B cells.
The intestinal immune system plays a key role in human beings. It is the largest human immune organ. Since the gut is continuously exposed to a lot intestinal bacteria and dietary protein antigens, it needs a defense mechanism to protect the body. It is an interesting topic for the intestinal immune system to recognize harmful bacteria but not to overreact to food protein antigens. The distribution of intestinal antigen-presenting cells is well documented in experimental animals such as mice. However, it is still unknown for the distribution of intestinal antigen-presenting cells in humans. The aim of the present project is aimed to characterize the distribution of antigen-presenting cells in human gut. Using immunohistochemical staining, the types and location of human gut antigen-presenting cells are thoroughly studied.
The specimens were first prepared as frozen sections or paraffin sections. They were immunohistochemically stained to evaluate the types and location of antigen-presenting cells in the human gut. From 2002 to 2004, thirty-three samples were collected in Shin Kong Wu Ho-Su Memorial Hospital in Taipei. All samples were initially checked for morphology using H and E staining. Using 17 primary antibodies, immunohistochemical staining and double staining were performed to characterize T cells, monocytes, B cells, macrophages, dendritic cells, and their distribution.
Studies of immunohistochemical staining show: 1. Langerhans cells are mainly located in the skin. 2. T cells are found in the interfollicular area of Peyer's patches and lamina propria. 3. B cells are present in the follicle of Peyer's patches and lamina propria. 4. The distribution of monocytes, macrophages and histiocytes is similar in the lamina propria of ileum, colon and appendix. They are scattered in Peyer's patches. 5. Follicular dendritic cells are found in the germinal center of Peyer's patches and appendix. 6. CD35-positive dendritic cells are rich in appendix but weakly present in the lamina propria of ileum, colon and appendix. 7. CD83-positive dendritic cells are found in the lamina propria of ileum and colon.
Immunohistochemical staining can extensively characterize the cell surface markers. The present study has clearly relvealed the types and location of antigen-presenting cells in the human gut. However, further investigations are still needed to elucidate the mechanims underlying the induction of oral tolerance by human intestinal antigen-presenting cells.
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