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標題: 應用ISSR研究玉山杜鵑之族群遺傳變異
Genetic Variation of Rhododendron pseudochrysanthum Based on ISSR Markers
作者: 鄭之煒
Cheng, ChihWei
關鍵字: Rhododendron pseudochrysanthum;玉山杜鵑;genetic variation;ISSR;遺傳變異;ISSR
出版社: 生命科學系
根據台灣植物誌第二版,台灣原生杜鵑屬植物有15種,其中12種為特有種植物。玉山杜鵑(R. pseudochrysanthum Hayata)為台灣特有種杜鵑中,分布海拔最廣泛也是最高的杜鵑種類。以11個ISSR引子研究玉山杜鵑10個族群157個個體,共得到127個條帶,其中98個為多型性條帶(77.16%)。根據AMOVA分析結果顯示,玉山杜鵑10個族群在地區間之變方成分百分比佔總變方之20.49%(P<0.001),大於地區內族群間之變方成分百分比6.76%(P<0.001),而又小於族群內個體間之變方成分百分比72.75%(P<0.001)。而歸群分析與主座標分析結果與地理位置吻合,另外同地區的族群間彼此遺傳變異較小,顯示玉山杜鵑在族群間受到地理距離與海拔高低影響。在POPGENE分析方面,各族群基因歧異度值由0.186-0.294,總Nei’s基因歧異度為0.3371,遺傳分化指數(Gst)為0.2659,基因流(Nm)為1.38。由這些結果看來,玉山杜鵑在地區間及族群間分化顯著,此可能與其授粉昆蟲無法打破距離限制,而導致地區間族群無法有較頻繁的基因交流,以及適應不同環境所產生之方面性天擇有關。

According to the second edition of Flora of Taiwan, there are 15 species of Rhododendron in Taiwan and among them 12 are endemic. Rhododendron pseudochrysanthum has the widest altitudinal range in Taiwan. One hundred and fifty-seven individuals from ten populations were studied for ISSR variation. One hundred and twenty-seven bands were observed using 11 primers. Ninety-eight (77.16%) were polymorphic bands. AMOVA analysis revealed that the percentages of variance components attributable to variation among regions was 20.49% (p<0.001), among populations within regions was 6.76% (p<0.001), and among individuals within populations was 87.09% (p<0.001). Results of cluster analysis and principal coordinate analysis are consistent with geographic locations, moreover, genetic variations among populations in the same region are smaller, indicating that the genetic variations of Rhododendron pseudochrysanthum among populations are attributable to distance and altitudinal differences among populations. POPGENE analysis revealed that the total gene diversity (H) of the species was 0.3371 and the gene diversities of populations ranged from 0.186 to 0.294. The population genetic diversity index (Gst) was 0.2659 while the gene flow index (Nm) was 1.38. The result indicated that there was significant genetic difference among populations. The infrequent gene flow among populations due to limited flying distance of its pollinators and the directional natural selection under different environmental conditions may explain the observed genetic differentiation among populations.
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