Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/22029
標題: Xanthomonas campestris pv.campestris 17 mopB 突變株的菌體集結現象之探討
Aggregate formation in the mopB mutant of xanthomonas campestris pv.campestris
作者: 蔡哲豪
Tsai, Che-Hao
關鍵字: xanthomonas;黃單胞桿菌;biofilm;black rot;生物膜;黑腐病
出版社: 分子生物學研究所
引用: 蕭懿民. (2003) 十字花科黑腐病菌蛋白酶基因 prt1 與纖維酶基因 engA 之轉 錄調控研究. 國立中興大學分子生物研究所博士論文. 陳智華. (2004) 十字花科黑腐病菌 Clp 蛋白之自我調控及對啟動子調控之研 究. 國立中興大學分子生物研究所研究生論文進度期中報告研究計劃書. 吳杰浩. (2005) Xanthomonas campestris pv. campestris噬菌體ΦL7 的寄主吸收附座膜蛋白質之尋找與探討. 國立中興大學分子生物研究所論文. 張惠雯.(2005) Xanthomonas campestris pv. campestris 主導次要果膠酶 PelB 的基因之特性. 國立中興大學分子生物研究所碩士論文. 王証鴻. (2007) Stenotrophomonas maltophilia 染色體外DNA 之探討. 國立中興大學分子生物研究所論文. Andrade, M. O., Alegria, M. C., Guzzo, C. R., Docena C., Rosa M. C., Ramos C. H. & Farah, C. S. (2006) The HD-GYP domain of RpfG mediates a direct linkage between the Rpf quorum-sensing pathway and a subset of diguanylate cyclase proteins in the phytopathogen Xanthomonas axonopodis pv citri. Mol Microbiol 62: 537-551. Barber, C. E., Tang J. L., Feng J. X., Pan M. Q., Wilson T. J., Slater H., J. M., Dow P. Williams & Daniels, M. J. (1997) A novel regulatory system required for pathogenicity of Xanthomonas campestris is mediated by a small diffusible signal molecule. Mol Microbiol 24: 555-566. Barrios, A. F., Zuo R., Ren D. & Wood, T. K. (2006) Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and conjugation plasmids abolish motility. Biotechnol Bioeng 93: 188-200. Becker, A., Katzen, F., Puhler, A. & Ielpi, L. (1998) Xanthan gum biosynthesis and application: a biochemical/genetic perspective. Appl Microbiol Biotechnol 50, 145-152. Birnboim, H. C. & Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513-1523. Boles, B. R. & Horswill, A. R. (2008) Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog 4: e1000052. Bond, P. J., Faraldo-Gomez J. D., Deol ,S. S. & Sansom, M. S. (2006) Membrane protein dynamics and detergent interactions within a crystal: a simulation study of OmpA. Proc Natl Acad Sci U S A 103: 9518-9523. Boon, C., Deng Y., Wang L. H., He Y., Xu J. L., Fan Y., Pan S. Q. & Zhang, L. H. (2008) A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition. ISME J 2: 27-36. Braun, V. & Schmitz, G. (1980) Excretion of a protease by Serratia marcescens. Arch Microbol 124: 55-61 Braunagel, S. C. & Benedik ,M. J. (1990) The metalloprotease gene of Serratia marcescens strain SM6. Mol Gen Genet 222: 446-451. Bromke, B.J. & Hammel, J.M. (1979) Regulation of extracellular protase formation by Serratia marcescens. Can J Microbiol 25:47-52. Chan, J. W. & Goodwin, P. H. (1999) The molecular genetics of virulence of Xanthomonas campestris. Biotechnol Adv 17, 489-508. Chatterjee, S. & Sonti, R. V. (2002) rpfF mutants of Xanthomonas oryzae pv. oryzae are deficient for virulence and growth under low iron conditions. Mol Plant Microbe Interact 15: 463-471. Chen, L. Y., D. Y. Chen, J. Miaw & Hu, N. T. (1996) XpsD, an outer membrane protein required for protein secretion by Xanthomonas campestris pv. campestris, forms a multimer. J Biol Chem 271: 2703-2708. Chen, Y., Shiue, S. J., Huang, C. W., Chang, J. L., Chien, Y. L., Hu, N. T. & Chan, N. L. (2005) Structure and function of the XpsE N-terminal domain, an essential component of the Xanthomonas campestris type II secretion system. J Biol Chem 280, 42356-42363. Chou, F. L., Chou, H. C., Lin, Y. S., Yang, B. Y., Lin, N. T., Weng, S. F. & Tseng, Y. H. (1997). The Xanthomonas campestris gumD gene required for synthesis of xanthan gum is involved in normal pigmentation and virulence in causing black rot. Biochem Biophys Res Commun 233, 265-269. Claggett, S. B., Grabar T. B., Dunn S. D. & Cain, B. D. (2007) Functional incorporation of chimeric b subunits into F1Fo ATP synthase. J Bacteriol 189: 5463-5471. Crossman, L. & Dow, J. M. (2004) Biofilm formation and dispersal in Xanthomonas campestris. Microbes Infect 6: 623-629. Daniels, M. J., Barber, C. E., Turner, P. C., Cleary, W. G. & Sawczyc, M. K. (1984a) Isolation of mutants of Xanthomonas campestris pv. campestris showing altered pathogenicity. J Gen Microbiol 130, 2447-2455. Daniels, M. J., Barber, C. E., Turner, P. C., Sawczyc, M. K., Byrde, R. J. W. & Fielding, A. H. (1984b) Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1. Embo J 3, 3323-3328. Daniels, M. J., Collinge, D. B., Dow, J. M., Osbourn, A. E. & Roberts, I. N. (1987) Molecular biology of the interaction of Xanthomonas campestris with plant. Plant Physiol Biochem 25, 353-359. de Crecy-Lagard, V., Glaser, P., Lejeune, P., Sismeiro, O., Barber, C. E., Daniels, M. J. & Danchin, A. (1990) A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity. J Bacteriol 172, 5877-5883. Dong, Q. & Ebright, R. H. (1992) DNA binding specificity and sequence of Xanthomonas campestris catabolite gene activator protein-like protein. J Bacteriol 174, 5457-5461. Dow, J. M., Feng, J. X., Barber, C. E., Tang, J. L. & Daniels, M. J. (2000) Novel genes involved in the regulation of pathogenicity factor production within the rpf gene cluster of Xanthomonas campestris. Microbiology 146 ( Pt 4), 885-891. Dow, J. M., Milligan, D. E., Jamieson, L., Barber, C. E. & Daniels, M. J. (1989) Molecular cloning of a polygalacturonate lyase gene from Xanthomonas campestris pv. campestris and role of the gene product in pathogenicity. Physiol Mol Plant Pathol 35, 113-120. Dow, J. M., Scofield, G., Trafford, K., Turner, P. C. & Daniels, M. J. (1987) A gene cluster in Xanthomonas campestris pv. campestris required for pathogenicity controls the excretion of polygalacturonate lyase and other enzymes. Physiol Mol Plant Pathol 31, 261-271. Dow, J. M., Clarke B. R., Milligan D. E., Tang J. L. & Daniels, M. J. (1990) Extracellular proteases from Xanthomonas campestris pv. campestris, the black rot pathogen. Appl Environ Microbiol 56: 2994-2998. Dow, J. M., Crossman L., Findlay K., He Y. Q., Feng J. X. & Tang, J. L. (2003) Biofilm dispersal in Xanthomonas campestris is controlled by cell-cell signaling and is required for full virulence to plants. Proc Natl Acad Sci U S A 100: 10995-11000. Dow, J. M., Fouhy Y., Lucey J. F. & Ryan, R. P. (2006) The HD-GYP domain, cyclic di-GMP signaling, and bacterial virulence to plants. Mol Plant Microbe Interact 19: 1378-1384. Evaces, G.N. & Jeffries, C.D. ( 1963) Isolation and properties of an extracellular nuclease of Serratia marcescens, J Bacteriol 85: 273-278. Ferreira, M. I., Ferro, E. F., Formighieri, M. C., Franco, C. C., Greggio, A., Gruber, A. M., Katsuyama, L. T., Kishi, R. P., Leite, E. G., Lemos, M. V., Lemos, E. C., Locali, M. A., Machado, A. M., Madeira, N. M., Martinez-Rossi, E. C., Martins, J., Meidanis, C. F., Menck, C. Y., Miyaki, D. H., Moon, L. M., Moreira, M. T., Novo, V. K., Okura, M. C., Oliveira, V. R., Oliveira, H. A., Pereira, A., Rossi, J. A., Sena, C., Silva, R. F. de Souza, L. A., Spinola, M. A., Takita, R. E., Tamura, E. C., Teixeira, R. I., Tezza, M. Trindade dos Santos, D., Truffi, S. M., Tsai, F. F., White, J. C. Setubal & Kitajima,J. P. (2002) Comparison of the genomes of two Xanthomonas pathogens with differing host specificities. Nature 417: 459-463. Francetic, O., Belin, D., Badaut, C. & Pugsley, A. P. (2000) Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion. Embo J 19, 6697-6703. Fronzes, R., Weimann T., Vaillier J., Velours J. & Brethes, D. (2006) The peripheral stalk participates in the yeast ATP synthase dimerization independently of e and g subunits. Biochemistry 45: 6715-6723. Greie, J. C., Heitkamp T. & Altendorf, K. (2004) The transmembrane domain of subunit b of the Escherichia coli F1F(O) ATP synthase is sufficient for H(+)-translocating activity together with subunits a and c. Eur J Biochem 271: 3036-3042. Harding, N. E., Cleary, J. M., Cabanas, D. K., Rosen, I. G. & Kang, K. S. (1987) Genetic and physical analyses of a cluster of genes essential for xanthan gum biosynthesis in Xanthomonas campestris. J Bacteriol 169, 2854-2861. Hayat, M. A. & Mancarella, D. A. (1995) Nucleoid proteins. Micron 26, 461-480. He, Y. W., M. Xu, K. Lin, Y. J. Ng, C. M. Wen, L. H. Wang, Z. D. Liu, H. B. Zhang, Y. H. Dong, J. M. Dow & Zhang, L. H. (2006) Genome scale analysis of diffusible signal factor regulon in Xanthomonas campestris pv. campestris: identification of novel cell-cell communication-dependent genes and functions. Mol Microbiol 59: 610-622. He, Y. W., A. Y. Ng, M. Xu, K. Lin, L. H. Wang, Y. H. Dong & Zhang, L. H. (2007) Xanthomonas campestris cell-cell communication involves a putative nucleotide receptor protein Clp and a hierarchical signalling network. Mol Microbiol 64: 281-292. He, Y. W. & Zhang, L. H. (2008) Quorum sensing and virulence regulation in Xanthomonas campestris. FEMS Microbiol Rev 32: 842-857. Higgins, C. F., Hinton, J. C., Hulton, C. S., Owen-Hughes, T., Pavitt, G. D. & Seirafi, A. (1990) Protein H1: a role for chromatin structure in the regulation of bacterial gene expression and virulence? Mol Microbiol 4, 2007-2012. Hsiao, Y. M., Liao, H. Y., Lee, M. C., Yang, T. C. & Tseng, Y. H. (2005) Clp upregulates transcription of engA gene encoding a virulence factor in Xanthomonas campestris by direct binding to the upstream tandem Clp sites. FEBS Lett 579, 3525-3533. Ish-Horowicz, D. & Burke, J.F. (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9, 2989-2998. James, V. & Hugouvieux-Cotte-Pattat, N. (1996) Regulatory systems modulating the transcription of the pectinase genes of Erwinia chrysanthemi are conserved in Escherichia coli. Microbiology 142 ( Pt 9), 2613-2619. Kasimoglu, E., Park S. J., Malek J., Tseng C. P. & Gunsalus, R. P. (1996) Transcriptional regulation of the proton-translocating ATPase (atpIBEFHAGDC) operon of Escherichia coli: control by cell growth rate. J Bacteriol 178: 5563-5567. Kjaergaard, K., Schembri M. A., Ramos C., Molin S. & Klemm, P. (2000) Antigen 43 facilitates formation of multispecies biofilms. Environ Microbiol 2: 695-702. Kohler, T., Curty, L. K., Barja, F., van Delden, C. & Pechere, J. C. (2000) Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili. J Bacteriol 182, 5990-5996. Kolb, A., Busby, S., Buc, H., Garges, S. & Adhya, S. (1993) Transcriptional regulation by cAMP and its receptor protein. Annu Rev Biochem 62, 749-795. Lee, T. C., Chen, S. T., Lee, M. C., Chang, C. M., Chen, C. H., Weng, S. F. & Tseng, Y. H. (2001) The early stages of filamentous phage Φ Becker, A., Katzen, F., Puhler, A. & Ielpi, L. (1998) Xanthan gum biosynthesis and application: a biochemical/genetic perspective. Appl Microbiol Biotechnol 50, 145-152. Birnboim, H. C. & Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513-1523. Boles, B. R. & Horswill, A. R. (2008) Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog 4: e1000052. Bond, P. J., Faraldo-Gomez J. D., Deol ,S. S. & Sansom, M. S. (2006) Membrane protein dynamics and detergent interactions within a crystal: a simulation study of OmpA. Proc Natl Acad Sci U S A 103: 9518-9523. Boon, C., Deng Y., Wang L. H., He Y., Xu J. L., Fan Y., Pan S. Q. & Zhang, L. H. (2008) A novel DSF-like signal from Burkholderia cenocepacia interferes with Candida albicans morphological transition. ISME J 2: 27-36. Braun, V. & Schmitz, G. (1980) Excretion of a protease by Serratia marcescens. Arch Microbol 124: 55-61 Braunagel, S. C. & Benedik ,M. J. (1990) The metalloprotease gene of Serratia marcescens strain SM6. Mol Gen Genet 222: 446-451. Bromke, B.J. & Hammel, J.M. (1979) Regulation of extracellular protase formation by Serratia marcescens. Can J Microbiol 25:47-52. Chan, J. W. & Goodwin, P. H. (1999) The molecular genetics of virulence of Xanthomonas campestris. Biotechnol Adv 17, 489-508. Chatterjee, S. & Sonti, R. V. (2002) rpfF mutants of Xanthomonas oryzae pv. oryzae are deficient for virulence and growth under low iron conditions. Mol Plant Microbe Interact 15: 463-471. Chen, L. Y., D. Y. Chen, J. Miaw & Hu, N. T. (1996) XpsD, an outer membrane protein required for protein secretion by Xanthomonas campestris pv. campestris, forms a multimer. J Biol Chem 271: 2703-2708. Chen, Y., Shiue, S. J., Huang, C. W., Chang, J. L., Chien, Y. L., Hu, N. T. & Chan, N. L. (2005) Structure and function of the XpsE N-terminal domain, an essential component of the Xanthomonas campestris type II secretion system. J Biol Chem 280, 42356-42363. Chou, F. L., Chou, H. C., Lin, Y. S., Yang, B. Y., Lin, N. T., Weng, S. F. & Tseng, Y. H. (1997). The Xanthomonas campestris gumD gene required for synthesis of xanthan gum is involved in normal pigmentation and virulence in causing black rot. Biochem Biophys Res Commun 233, 265-269. Claggett, S. B., Grabar T. B., Dunn S. D. & Cain, B. D. (2007) Functional incorporation of chimeric b subunits into F1Fo ATP synthase. J Bacteriol 189: 5463-5471. Crossman, L. & Dow, J. M. (2004) Biofilm formation and dispersal in Xanthomonas campestris. Microbes Infect 6: 623-629. Daniels, M. J., Barber, C. E., Turner, P. C., Cleary, W. G. & Sawczyc, M. K. (1984a) Isolation of mutants of Xanthomonas campestris pv. campestris showing altered pathogenicity. J Gen Microbiol 130, 2447-2455. Daniels, M. J., Barber, C. E., Turner, P. C., Sawczyc, M. K., Byrde, R. J. W. & Fielding, A. H. (1984b) Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1. Embo J 3, 3323-3328. Daniels, M. J., Collinge, D. B., Dow, J. M., Osbourn, A. E. & Roberts, I. N. (1987) Molecular biology of the interaction of Xanthomonas campestris with plant. Plant Physiol Biochem 25, 353-359. de Crecy-Lagard, V., Glaser, P., Lejeune, P., Sismeiro, O., Barber, C. E., Daniels, M. J. & Danchin, A. (1990) A Xanthomonas campestris pv. campestris protein similar to catabolite activation factor is involved in regulation of phytopathogenicity. J Bacteriol 172, 5877-5883. Dong, Q. & Ebright, R. H. (1992) DNA binding specificity and sequence of Xanthomonas campestris catabolite gene activator protein-like protein. J Bacteriol 174, 5457-5461. Dow, J. M., Feng, J. X., Barber, C. E., Tang, J. L. & Daniels, M. J. (2000) Novel genes involved in the regulation of pathogenicity factor production within the rpf gene cluster of Xanthomonas campestris. Microbiology 146 ( Pt 4), 885-891. Dow, J. M., Milligan, D. E., Jamieson, L., Barber, C. E. & Daniels, M. J. (1989) Molecular cloning of a polygalacturonate lyase gene from Xanthomonas campestris pv. campestris and role of the gene product in pathogenicity. Physiol Mol Plant Pathol 35, 113-120. Dow, J. M., Scofield, G., Trafford, K., Turner, P. C. & Daniels, M. J. (1987) A gene cluster in Xanthomonas campestris pv. campestris required for pathogenicity controls the excretion of polygalacturonate lyase and other enzymes. Physiol Mol Plant Pathol 31, 261-271. Dow, J. M., Clarke B. R., Milligan D. E., Tang J. L. & Daniels, M. J. (1990) Extracellular proteases from Xanthomonas campestris pv. campestris, the black rot pathogen. Appl Environ Microbiol 56: 2994-2998. Dow, J. M., Crossman L., Findlay K., He Y. Q., Feng J. X. & Tang, J. L. (2003) Biofilm dispersal in Xanthomonas campestris is controlled by cell-cell signaling and is required for full virulence to plants. Proc Natl Acad Sci U S A 100: 10995-11000. Dow, J. M., Fouhy Y., Lucey J. F. & Ryan, R. P. (2006) The HD-GYP domain, cyclic di-GMP signaling, and bacterial virulence to plants. Mol Plant Microbe Interact 19: 1378-1384. Evaces, G.N. & Jeffries, C.D. ( 1963) Isolation and properties of an extracellular nuclease of Serratia marcescens, J Bacteriol 85: 273-278. Ferreira, M. I., Ferro, E. F., Formighieri, M. C., Franco, C. C., Greggio, A., Gruber, A. M., Katsuyama, L. T., Kishi, R. P., Leite, E. G., Lemos, M. V., Lemos, E. C., Locali, M. A., Machado, A. M., Madeira, N. M., Martinez-Rossi, E. C., Martins, J., Meidanis, C. F., Menck, C. Y., Miyaki, D. H., Moon, L. M., Moreira, M. T., Novo, V. K., Okura, M. C., Oliveira, V. R., Oliveira, H. A., Pereira, A., Rossi, J. A., Sena, C., Silva, R. F. de Souza, L. A., Spinola, M. A., Takita, R. E., Tamura, E. C., Teixeira, R. I., Tezza, M. Trindade dos Santos, D., Truffi, S. M., Tsai, F. F., White, J. C. Setubal & Kitajima,J. P. (2002) Comparison of the genomes of two Xanthomonas pathogens with differing host specificities. Nature 417: 459-463. Francetic, O., Belin, D., Badaut, C. & Pugsley, A. P. (2000) Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion. Embo J 19, 6697-6703. Fronzes, R., Weimann T., Vaillier J., Velours J. & Brethes, D. (2006) The peripheral stalk participates in the yeast ATP synthase dimerization independently of e and g subunits. Biochemistry 45: 6715-6723. Greie, J. C., Heitkamp T. & Altendorf, K. (2004) The transmembrane domain of subunit b of the Escherichia coli F1F(O) ATP synthase is sufficient for H(+)-translocating activity together with subunits a and c. Eur J Biochem 271: 3036-3042. Harding, N. E., Cleary, J. M., Cabanas, D. K., Rosen, I. G. & Kang, K. S. (1987) Genetic and physical analyses of a cluster of genes essential for xanthan gum biosynthesis in Xanthomonas campestris. J Bacteriol 169, 2854-2861. Hayat, M. A. & Mancarella, D. A. (1995) Nucleoid proteins. Micron 26, 461-480. He, Y. W., M. Xu, K. Lin, Y. J. Ng, C. M. Wen, L. H. Wang, Z. D. Liu, H. B. Zhang, Y. H. Dong, J. M. Dow & Zhang, L. H. (2006) Genome scale analysis of diffusible signal factor regulon in Xanthomonas campestris pv. campestris: identification of novel cell-cell communication-dependent genes and functions. Mol Microbiol 59: 610-622. He, Y. W., A. Y. Ng, M. Xu, K. Lin, L. H. Wang, Y. H. Dong & Zhang, L. H. (2007) Xanthomonas campestris cell-cell communication involves a putative nucleotide receptor protein Clp and a hierarchical signalling network. Mol Microbiol 64: 281-292. He, Y. W. & Zhang, L. H. (2008) Quorum sensing and virulence regulation in Xanthomonas campestris. FEMS Microbiol Rev 32: 842-857. Higgins, C. F., Hinton, J. C., Hulton, C. S., Owen-Hughes, T., Pavitt, G. D. & Seirafi, A. (1990) Protein H1: a role for chromatin structure in the regulation of bacterial gene expression and virulence? Mol Microbiol 4, 2007-2012. Hsiao, Y. M., Liao, H. Y., Lee, M. C., Yang, T. C. & Tseng, Y. H. (2005) Clp upregulates transcription of engA gene encoding a virulence factor in Xanthomonas campestris by direct binding to the upstream tandem Clp sites. FEBS Lett 579, 3525-3533. Ish-Horowicz, D. & Burke, J.F. (1981) Rapid and efficient cosmid cloning. Nucleic Acids Res 9, 2989-2998. James, V. & Hugouvieux-Cotte-Pattat, N. (1996) Regulatory systems modulating the transcription of the pectinase genes of Erwinia chrysanthemi are conserved in Escherichia coli. Microbiology 142 ( Pt 9), 2613-2619. Kasimoglu, E., Park S. J., Malek J., Tseng C. P. & Gunsalus, R. P. (1996) Transcriptional regulation of the proton-translocating ATPase (atpIBEFHAGDC) operon of Escherichia coli: control by cell growth rate. J Bacteriol 178: 5563-5567. Kjaergaard, K., Schembri M. A., Ramos C., Molin S. & Klemm, P. (2000) Antigen 43 facilitates formation of multispecies biofilms. Environ Microbiol 2: 695-702. Kohler, T., Curty, L. K., Barja, F., van Delden, C. & Pechere, J. C. (2000) Swarming of Pseudomonas aeruginosa is dependent on cell-to-cell signaling and requires flagella and pili. J Bacteriol 182, 5990-5996. Kolb, A., Busby, S., Buc, H., Garges, S. & Adhya, S. (1993) Transcriptional regulation by cAMP and its receptor protein. Annu Rev Biochem 62, 749-795. Lee, T. C., Chen, S. T., Lee, M. C., Chang, C. M., Chen, C. H., Weng, S. F. & Tseng, Y. H. (2001) The early stages of filamentous phage Becker, A., Katzen, F., Puhler, A. & Ielpi, L. (1998) Xanthan gum biosynthesis and application: a biochemical/genetic perspective. Appl Microbiol Biotechnol 50, 145-152. Birnboim, H. C. & Doly, J. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513-1523. Boles, B. R. & Horswill, A. R. (2008) Agr-mediated dispersal of Staphylococcus aureus biofilms. PLoS Pathog 4: e1000052. Bond, P. J., Faraldo-Gomez J. D., Deol ,S. S. & Sansom, M. S. 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(2002) rpfF mutants of Xanthomonas oryzae pv. oryzae are deficient for virulence and growth under low iron conditions. Mol Plant Microbe Interact 15: 463-471. Chen, L. Y., D. Y. Chen, J. Miaw & Hu, N. T. (1996) XpsD, an outer membrane protein required for protein secretion by Xanthomonas campestris pv. campestris, forms a multimer. J Biol Chem 271: 2703-2708. Chen, Y., Shiue, S. J., Huang, C. W., Chang, J. L., Chien, Y. L., Hu, N. T. & Chan, N. L. (2005) Structure and function of the XpsE N-terminal domain, an essential component of the Xanthomonas campestris type II secretion system. J Biol Chem 280, 42356-42363. Chou, F. L., Chou, H. C., Lin, Y. S., Yang, B. Y., Lin, N. T., Weng, S. F. & Tseng, Y. H. (1997). The Xanthomonas campestris gumD gene required for synthesis of xanthan gum is involved in normal pigmentation and virulence in causing black rot. Biochem Biophys Res Commun 233, 265-269. Claggett, S. B., Grabar T. B., Dunn S. D. & Cain, B. D. 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摘要: 
十字花科黑腐病菌 Xanthomonas campestris pv. campestris (Xcc) 為革蘭氏陰性菌,能感染十字花科作物造成黑腐病 (black rot)。 此菌所分泌的胞外黏多醣 (exopolysaccharide, EPS),胞外酵素,以及與hrp (hypersensitive response and pathogenicity)、及 avr (avirulence) 相關之基因等均為重要致病因子。 先前研究顯示Xcc之 mopB 基因被破壞後, EPS 下降,胞外酵素 polygalacturonase分泌減少,病原性喪失。 此外,於 mopB 突變株的液體培養基中觀察到菌體集結顆粒, Xcc Diffusible Signal Factor (DSF) 基因突變株的菌體集結現象相似。本論文之目的為尋找能將 mopB 突變株產生的集結物分散之物質並了解其機制,於Xcc DSF 突變株培養液中添加 DSF 能使集結菌體擴散成為均勻狀態,但在 mopB 突變株中添加 DSF 則無此功能。 推測, mopB 突變株培養液中形成的集結顆粒與生物膜組成不同。 然而,於 mopB 突變株之培養液中,添加 Serratia marcescens (Sm) 上清液,可使菌體集結顆粒分散均勻。 進一步探討結果顯示 Sm 上清液的 protease 具有分散Xcc之 mopB 突變株集結之功能。 此外,分析野生株與mopB 突變株之胞內蛋白,發現當菌體集結時在 mopB 突變株中有些蛋白會被大量表現。 其中之疑似蛋白質為AtpF,因此,構築 atpF 突變株,並製備 AtpF 抗體,以了解 AtpF在集結現象中所扮演的功能及角色。結果發現,Xc17及 mopB 之 AtpF 隨時間增加並無顯著增加之差異。 另一方面,利用二維電泳比較 Xc17 及 mopB 突變株胞外蛋白,發現有一些具有明顯差異之蛋白可能與集結相關,值得繼續探討。

The Gram-negative plant pathogen Xanthomonas camperstris pv. camperstris (Xcc) causes black rot in crucifers. The virulence of this bacterium is mainly related to its ability to produce a large mount of exopolysaccharride (EPS, also called xanthan gum) , extracellular enzymes, and other virulence factor. In addition to these substances, Avr (avirulence) protein and hrp genes encoded Type III secretary pathway are also essential for virulence. The previous study showed that the Xcc mopB mutant caused the EPS production decreasing, and lost the pathogenesis of this bacterium to plants. In addition, while the wild type grew in a dispersed planktonic fashion, aggregate were in the mopB mutant. This phenotype is similar to that in the rpfF mutant. Addition of DSF triggered dispersion of the aggregates present in rpfF mutant but adding DSF to the mopB mutant dispersal has no effect. It is suggested that the structure and mechanism of aggregate are different in between mopB and rpfF mutant. However, addition of supernatant of Serratia marcescens (Sm) caused dispersal of the aggregates of mopB mutant. The SmT43 protease were purified and added to mopB mutant, the result showed the aggregates were dispersed. Moreover, base on the analysis of SDS-PAGE, the Xc17 (wild-type) and mopB mutant intracellular protein profiles were compared. One of the overexpressed profiles were identified as AtpF. AtpF Antibody were generated to find out the expression of AtpF in Xc17 and mopB mutant. when the mopB mutant form the aggregates. On the other hand, the extracellular proteins of Xc17 and mopB mutant were isolated and analyzed by 2D electrophoresis to find the possible factors related to the aggregates.
URI: http://hdl.handle.net/11455/22029
其他識別: U0005-1708200915143900
Appears in Collections:分子生物學研究所

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