Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/22143
標題: 立枯絲核菌(Rhizoctonia solani K hn) AG-4融合群中質體DNA限 制▇圖譜及選殖之研究
Mapping and cloning of linear plasmid DNA in Rhizoctonia solani K hn AG-4
作者: 李建勳
Lee, Chien-shiun
關鍵字: Rhizoctonia solani;立枯絲核菌;linear plasmid;mapping;cloning;線形質體;限制▇圖譜;選殖
出版社: 植物學系
摘要: 
質體 pCHU341是存在於Rhizoctonia solani (strain CHU341) 中的直線
型分子,分子量約 2.6 kb 。本研究目的乃分析 pCHU341之限制▇圖譜及
利用 pCHU341限制▇片段構築一個新轉形載體。質體 pCHU341限制▇切位
,乃以包含質體之總體 DNA限制▇切位做初步判斷。正確的質體限制▇切
位則用限制▇(AvaI 、BamHI 、BglII、EcoRI 、 HindIII、SacI、 SphI
及 SmaI) 切割純化的質體得到。利用質體限制▇切位並配合分殖技術(
subcloning)可製作質體 pCHU341之限制▇圖譜。 pCHU341經限制▇切割
所得之片段可利用選殖作用(cloning)在Escherichia coli中進行大量複
製。選殖片段可被黏接於轉形載體 pUCH1,此新構築的真菌轉形載體有助
於轉形實驗及其他有關質體 pCHU341 功能之研究。

Plasmid pCHU341 is a linear element of Rhizoctonia solani
CHU341 the molecular weight of 2.6 Kb . The aim of this study
was tried to analyze the restriction map of plasmid pCHU341 and
construct a new transformation vector bearing a restriction
fragment from pCHU341 . The cut sites of plasmid pCHU314 were
deduced roughly from the restriction fragments of total DNA
containing plasmid digested with various enzymes . The cut
sites of plasmid were deduced from restriction fragments of
purified pCHU341 digested with restriction enzymes ( AvaI、
BamHI、 BglII、 EcoRI、 HindIII、 SacI、 SphI and SmaI ) .
The restriction map of pCHU341 was made by cut sites of
plasmid associated with subcloning technique . The
restriction fragments of pCHU341 can be subcloned by a
cloning vector in Escherichia coli . The cloned restriction
fragments were fused to a fungal transformation vector
pUCH1 . The newly constructed vector derived from pUCH1
bearing the restriction fragment of pCHU341 is useful
for transformation experiments and another studies about the
function of pCHU341 .
URI: http://hdl.handle.net/11455/22143
Appears in Collections:生命科學系所

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