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|標題:||A Comparative Proteomic Analysis of Rat Bronchoalveolar Lavage Fluid in Response to ZnO Nanoparticles Exposure
|關鍵字:||proteomic analysis;蛋白質體分析;proteome;zinc oxide nanoparticles;biomarker;LC-MS/MS;two-dimentional electrophoresis;蛋白質體;奈米氧化鋅;生物標記;液相層析串聯式質譜儀;二維膠體電泳||出版社:||分子生物學研究所||引用:||Aebersold R, Goodlett DR. (2001) Mass spectrometry in proteomics. Chemical Review, 101: 269-295. Andersson A, Ritz C, Lindgren D, Eden P, Lassen C, et al. (2007) Microarray-based classification of a consecutive series of 121 childhood acute leukemias: prediction of leukemic and genetic subtype as well as of minimal residual disease status. Leukemia, 21: 1198-1203. Aufderheide M, Costa DL, Devlin R, Feron V, Harkema JR, et al. (2005) Experimental Assessment of the Toxicological Effects of Inhaled Complex Mixtures on the Respiratory System, 23-25 April 2005, Barcelona, Spain. Summary and conclusions of the review committee. Experimental and toxicologic pathology, 1: 239-243. Bachmann R, Laurell CB. (1963) Electrophoretic and immunologic classification of M-components in serum. 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Zinc oxide (ZnO) nanoparticles have been widely used in producing ofrubber, tire, paint, and cosmetics. The size are ranged 1~100 nm. With the advancement of nanotechnology, the potential health effects of nanoparticles were very concerns. For toxicity studies of ZnO nanoparticles, the toxicity studies of ZnO nanoparticles were to assess lung toxicity using rats by a furnace tube airflow system. Health rats were exposed to 35nm and 250nm nanoparticles at similar surface area concentration for 6 hr respectively (high, moderate and low dose, n=6). After 24 hr exposure, rats were sacrificed and bronchoalveolar lavage fluid (BALF) were collected.
For the aim that investigated changes in rat bronchoalveolar lavage fluid proteins associated with ZnO nanoparticles, we adopted a proteomics approach to identify the bronchoalveolar lavage fluid (BALF) proteins changes by 2-D electrophoresis and quadrupole-time of flight mass spectrometer (Q-TOF MS). We found that 87 spots exhibited significant changes between high, moderate and low-dose groups of exposure to 35 nm ZnO nanoparticles and control by 2-D electrophoresis assay. Similarly, We found that 134 spots exhibited significant changes between high, moderate and low-dose groups of exposure to 250nm ZnO nanoparticles and control.
The proteins from the individual spots were identified by LC- Q-TOF MS and sequence database searching. From these 87 protein gel spots, 52 unique proteins were identified in 35 nm groups. These proteins involve antioxidative protein, ER stress, immune response, anticoagulation and heme metabolism, energy and nucleotide metabolism, lung mucous-association, signal transduction, cytoskeleton, and other proteins (miscellaneous). In 52 unique proteins, 12 proteins were a significant increase and dose-dependent between 35 nm nanoparticles groups. And 4 proteins with a significant increase decrease and dose-dependent between 35 nm nanoparticles groups. To further evaluate these proteins, 2 proteins were reported to associate with pulmonary fibrosis by previous studies, including protein Plunc and gelsolin. And 3 proteins were reported to associate with hypoxia by previous studies, including annexin A5, biliverdin reductase, phosphatidylethanolamine-binding protein 1 (PEBP1).
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