Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/22490
標題: 開發大花咸豐草水萃物為第二型糖尿病健康食品之功能性探討
Evaluation of Bidens pilosa water extract as a food supplement for type 2 Diabetes
作者: 許曉茹
Hsu, Hsiao-Ju
關鍵字: type2 diabetes;第二型糖尿病;Bidens pilosa;food supplements;大花咸豐草;健康食品
出版社: 生命科學系所
引用: Alarcon-Aguilar FJ, Roman-Ramos R, Flores-Saenz JL, Aguirre-Garcia F (2002) Investigation on the hypoglycaemic effects of extracts of four Mexican medicinal plants in normal and alloxan-diabetic mice. Phytother Res 16: 383-386 Arakawa K, Ishihara T, Oku A, Nawano M, Ueta K, Kitamura K, Matsumoto M, Saito A (2001) Improved diabetic syndrome in C57BL/KsJ-db/db mice by oral administration of the Na(+)-glucose cotransporter inhibitor T-1095. British Journal of Pharmacology 132: 578-586 Berger J, Moller DE (2002) The mechanisms of action of PPARs. Annual Review of Medicine 53: 409-435 Chang CL, Chang SL, Lee YM, Chiang YM, Chuang DY, Kuo HK, Yang WC (2007) Cytopiloyne, a polyacetylenic glucoside, prevents type 1 diabetes in nonobese diabetic mice. J Immunol 178: 6984-6993 Chang CL, Kuo HK, Chang SL, Chiang YM, Lee TH, Wu WM, Shyur LF, Yang WC (2005) The distinct effects of a butanol fraction of Bidens pilosa plant extract on the development of Th1-mediated diabetes and Th2-mediated airway inflammation in mice. J Biomed Sci 12: 1-11 Chang MS, Oh MS, Kim do R, Jung KJ, Park S, Choi SB, Ko BS, Park SK (2006) Effects of Okchun-San, a herbal formulation, on blood glucose levels and body weight in a model of Type 2 diabetes. J Ethnopharmacol 103: 491-495 Cheng AY, Fantus IG (2005) Oral antihyperglycemic therapy for type 2 diabetes mellitus. CMAJ 172: 213-226 Chiang YM, Chang CL, Chang SL, Yang WC, Shyur LF (2007) Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator. J Ethnopharmacol 110: 532-538 Combettes MM (2006) GLP-1 and type 2 diabetes: physiology and new clinical advances. Curr Opin Pharmacol Derosa G, Gaddi AV, Piccinni MN, Salvadeo S, Ciccarelli L, Fogari E, Ghelfi M, Ferrari I, Cicero AF (2006) Differential effect of glimepiride and rosiglitazone on metabolic control of type 2 diabetic patients treated with metformin: a randomized, double-blind, clinical trial. Diabetes, obesity & metabolism 8: 197-205 Hammarstedt A, Andersson CX, Rotter Sopasakis V, Smith U (2005) The effect of PPARgamma ligands on the adipose tissue in insulin resistance. Prostaglandins, leukotrienes, and essential fatty acids 73: 65-75 Harrity T, Farrelly D, Tieman A, Chu C, Kunselman L, Gu L, Ponticiello R, Cap M, Qu F, Shao C, Wang W, Zhang H, Fenderson W, Chen S, Devasthale P, Jeon Y, Seethala R, Yang WP, Ren J, Zhou M, Ryono D, Biller S, Mookhtiar KA, Wetterau J, Gregg R, Cheng PT, Hariharan N (2006) Muraglitazar, a novel dual (alpha/gamma) peroxisome proliferator-activated receptor activator, improves diabetes and other metabolic abnormalities and preserves beta-cell function in db/db mice. Diabetes 55: 240-248 Irwin N, Green BD, Gault VA, Cassidy RS, O''Harte FP, Harriott P, Flatt PR (2006) Effects on glucose homeostasis and insulin secretion of long term activation of the glucose-dependent insulinotropic polypeptide (GIP) receptor by N-AcGIP(LysPAL37) in normal mice. Peptides 27: 893-900 Laakso M (2001) Insulin resistance and its impact on the approach to therapy of type 2 diabetes. Int J Clin Pract Suppl: 8-12 Lindsay JR, Duffy NA, McKillop AM, Ardill J, O''Harte FP, Flatt PR, Bell PM (2005) Inhibition of dipeptidyl peptidase IV activity by oral metformin in Type 2 diabetes. Diabet Med 22: 654-657 Liu K, Xu L, Szalkowski D, Li Z, Ding V, Kwei G, Huskey S, Moller DE, Heck JV, Zhang BB, Jones AB (2000) Discovery of a potent, highly selective, and orally efficacious small-molecule activator of the insulin receptor. Journal of Medicinal Chemistry 43: 3487-3494 Marles RJ, Farnsworth NR (1995) Antidiabetic plants and their active constituents. Phytomedicine 2: 137-189 Muller G (2000) The molecular mechanism of the insulin-mimetic/sensitizing activity of the antidiabetic sulfonylurea drug Amaryl. Molecular medicine (Cambridge, Mass 6: 907-933 Muller G, Satoh Y, Geisen K (1995) Extrapancreatic effects of sulfonylureas--a comparison between glimepiride and conventional sulfonylureas. Diabetes Research and Clinical Practice 28 Suppl: S115-137 Nguelefack TB, Dimo T, Mbuyo EP, Tan PV, Rakotonirina SV, Kamanyi A (2005) Relaxant effects of the neutral extract of the leaves of Bidens pilosa Linn on isolated rat vascular smooth muscle. Phytother Res 19: 207-210 Park MY, Lee KS, Sung MK (2005) Effects of dietary mulberry, Korean red ginseng, and banaba on glucose homeostasis in relation to PPAR-alpha, PPAR-gamma, and LPL mRNA expressions. Life Sciences 77: 3344-3354 Pfutzner A, Schondorf T, Seidel D, Winkler K, Matthaei S, Hamann A, Forst T (2006) Impact of rosiglitazone on beta-cell function, insulin resistance, and adiponectin concentrations: results from a double-blind oral combination study with glimepiride. Metabolism: clinical and experimental 55: 20-25 Taylor SI (1999) Deconstructing type 2 diabetes. Cell 97: 9-12 Tripathi BK, Srivastava AK (2006) Diabetes mellitus: complications and therapeutics. Med Sci Monit 12: RA130-147 Ubillas RP, Mendez CD, Jolad SD, Luo J, King SR, Carlson TJ, Fort DM (2000) Antihyperglycemic acetylenic glucosides from Bidens pilosa. Planta Med 66: 82-83 Uchida T, Nakamura T, Hashimoto N, Matsuda T, Kotani K, Sakaue H, Kido Y, Hayashi Y, Nakayama KI, White MF, Kasuga M (2005) Deletion of Cdkn1b ameliorates hyperglycemia by maintaining compensatory hyperinsulinemia in diabetic mice. Nat Med 11: 175-182 Zander M, Taskiran M, Toft-Nielsen MB, Madsbad S, Holst JJ (2001) Additive glucose-lowering effects of glucagon-like peptide-1 and metformin in type 2 diabetes. Diabetes Care 24: 720-725
摘要: 
糖尿病是一種常見的慢性疾病,主要分為第一型與第二型。臨床上常用的糖尿病藥物包括胰島素分泌刺激劑、胰島素增敏劑或碳水化合物酵素抑制劑。本研究目的在探討口服大花咸豐草水萃物於第二型糖尿病鼠上控制糖尿病的效果,並証實其促進胰島素分泌的能力,再利用野生型小鼠來進行安全性的實驗,以評估其發展為健康食品的可能性與利基。首先利用大花咸豐草水萃物對第二型糖尿病鼠進行短期降血糖試驗,發現在單次投與受試物的刺激下,大花咸豐草水萃物能顯著降血糖及增加血中胰島素。使用C57BL/6J野生型小鼠來評估大花咸豐草水萃物在正常鼠降空腹血糖的效果,發現無顯著的降血糖情形。接著利用第二型糖尿病鼠進行長期動物實驗的觀察,發現灌食不同濃度的大花咸豐草水萃物,除了可以有效控制餐後與空腹血糖值、糖化血色素及改善葡萄糖耐受性之外,亦可增加胰島素值的分泌,且不影響體重及進食量。利用Wistar大鼠胰島細胞來評估大花咸豐草水萃物是否直接促進胰島細胞分泌胰島素,結果顯示給予不同劑量的大花咸豐草水萃物後30分鐘,胰島素分泌比負控制組與正控制組 (Glimepiride) 顯著,且各組間呈現劑量相關的分泌情形,初步推測大花咸豐草水萃物能直接刺激自胰島細胞分泌胰島素。為證實大花咸豐草水萃物的降血糖效果主要屬於胰島素分泌刺激劑,因此用Streptozotocin 破壞小鼠的胰島細胞誘發其成為糖尿病鼠,再給予大花咸豐草水萃物刺激,發現失去降血糖功能,因此再次推論大花咸豐草水萃物降血糖的原因來自於刺激胰島細胞分泌胰島素。
綜合上述,大花咸豐草水萃物能直接刺激胰島細胞分泌胰島素,不論在短期或長期治療都有降血糖效果及抗糖尿病惡化,且無低血糖與增重的副作用。

Diabetes mellitus (DM) is a chronic metabolic disease. There are two major forms of diabetes: type 1 and type 2 diabetes .Current available oral anti-diabetic agents are insulin secretagogues, insulin sensitizers and α-glucosidase inhibitors. The purpose of this research is to evaluate the anti-diabetic effect of Bidens pilosa water extract (BPWE) in db/db mice and to study its likely mechanism. We first evaluated the short term blood glucose-lowing effect of BPWE in diabetes db/db mice. Our data showed that a single oral gavage BPWE could decrease postprandial blood glucose and increased serum insulin level significantly. Then we investigated the hypoglycemic effect of BPWE in normal C57BL/6J mice. This plant did not show any significant hypoglycemic effect. Mice daily received an oral gavage of BPWE at 10,50 and 250mg/kg body weight. Glimepiride at 2.5 mg/kg body wt (positive control), and ddH2O (negative control) for 28 days. Our results demonstrated that BPWE could significantly lower fasting blood glucose, postprandial blood glucose level, elevate the blood insulin level, improve glucose tolerance and decrease HbA1C level. We tested if BPWE could stimulate β cell to secrete insulin. We found that a single administration of BPWE at different dosages, obviously increased insulin release. To understand the mechanism by which BPWE could lower blood glucose, we also used STZ-induced diabetic mice to evaluate the effect of BPWE on blood glucose lowing. The results indicate that BPWE reduced blood glucose levels via its insulin-releasing effect on β cell.
Overall, this study showed that BPWE is able to treat type2 diabetes in db/db mice. It appeared that BPWE had, if any, little effect on weight gain. These data show promise for the development of BPWE as a functional food.
URI: http://hdl.handle.net/11455/22490
其他識別: U0005-0607200721044100
Appears in Collections:生命科學系所

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.