Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/22716
標題: 台灣四種中草藥-地筍、台灣黃岑、石香薷、生毛將軍玻璃質化法超低溫冷凍保存處理流程之探討
Study of Protocol on the Cryopreservation of Four Taiwan Medicinal Herbs - Lycopus lucidus Turcz., Scutellaria taiwanensis C.Y.Wu, Mosla chinensis Maxim., Blumea lacera (Burm.f.) DC. by Vitrification.
作者: 陳岱陽
Chen, Tai-Yang
關鍵字: cryopreservation;冷凍保存;medicinal herbs;vitrification;中草藥;玻璃化法
出版社: 生命科學系所
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摘要: 
本研究以台灣四種中草藥(地筍、台灣黃岑、石香薷、生毛將軍)的組織培養苗為材料,探討玻璃化法超低溫冷凍保存處理流程對存活率之影響。以不同濃度蔗糖培養基處理不同時間,配合不同PVS2處理時間,得到冷凍保存後最終存活率。結果顯示,地筍先以含0.3 M蔗糖之MS培養基健化30天後,再以冷凍保護劑LS處理60分鐘和PVS2處理180分鐘,存活率可達95.72%。台灣黃岑以含0.2 M蔗糖之1/2 MS培養基健化3天後,以冷凍保護劑LS處理30分鐘和PVS2處理80分鐘,存活率可達50%。石香薷以含0.2 M蔗糖之1/2 MS培養基健化7天後,以冷凍保護劑LS處理30分鐘和PVS2處理40分鐘,存活率可達71.52%。生毛將軍以含0.3 M蔗糖之MS培養基健化20天後,以冷凍保護劑LS處理60分鐘和PVS2處理80分鐘,存活率可達70%。
以地筍為材料進行生理分析測定,發現地筍經高濃度蔗糖長期預培養後之相對含水量、水勢、滲透潛勢遞減、可溶性糖類及蔗糖含量大量累積、可溶性蛋白含量都增加。此結果顯示,高糖培養導致地筍頂芽脫水並累積溶質,並提高冷凍保存後之存活率,故高糖預培養有助於提高滲透逆境之適應性及增加冷凍保存之保護性。
本實驗提供台灣四種中草藥玻璃質化法超低溫冷凍保存處理流程,以做為珍貴種原長期保存及永續利用之參考。

This research uses four Taiwanese medical herbs Lycopus lucidus Turcz., Scutellaria taiwanensis., Mosla chinensis Maxim., and Blumea hieraciifolia as materials to investigate the effects of survival rates of cryopreservation treated by vitrification. Plantlets of Lycopus lucidus Turcz. that have been cultured on MS medium containing 0.3 M sucrose for 30 days were treated with a loading solution (LS) for 60 minutes and dehydrated with a plant vitrification solution (PVS2) for 180 minutes. The results showed the best survival rates of 95.72%. Plantlets of Scutellaria taiwanensis which have been cultured on 1/2 MS medium containing 0.2 M sucrose for 3 days were treated with LS for 30 minutes and dehydrated with PVS2 for 80 minutes. The best survival rates of 50% was obtained. Plantlets of Mosla chinensis Maxim. which have been cultured on 1/2 MS medium containing 0.2 M sucrose for 7 days were treated with LS for 30 minutes and then dehydrated with PVS2 for 40 minutes. The best survival rates was 71.52%. Plantlets of Blumea hieraciifolia which have been cultured on MS medium containing 0.3 M sucrose for 20 days were treated with LS for 60 minutes and then dehydrated with PVS2 for 80 minutes, and the best survival rates was 70%.
Physiological analysis of Lycopus lucidus Turcz. showed reduced relative water content, water potential and osmotic potential reduce and increased soluble sugar content, sucrose content and soluble protein content. These results indicate that Lycopus lucidus Turcz. culturing with high concentrations of sucrose results in dehydration of shoot tips and accumulation of solutes. The preculture procedures render increasing survival rates after cryopreservation due to the acclimatization in osmotic stress and increase freezing-tolerance for cryopreservation.
This experiment suggests the protocol for four Taiwanese medical herbs cryopreservation by vitrification and can be served as a reference for long-term conservation of rare plant species.
URI: http://hdl.handle.net/11455/22716
其他識別: U0005-2008200813224300
Appears in Collections:生命科學系所

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