Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/22725
標題: 台灣原生藥用植物-高氏柴胡玻璃質化法超低溫冷凍保存流程之探討
Study of Protocol on the Cryopreservation of Taiwan Native Medicinal Herb - Bupleurum kaoi Liu, Chao et Chuang by Vitrification.
作者: 林維熙
Lin, Wei-hsi
關鍵字: Bupleurum kaoi Liu, Chao et Chuang;高氏柴胡;cryopreservation;vitrification;超低溫冷凍保存;玻璃質化法
出版社: 生命科學系所
引用: 杜景红、左廷兵、李鳳蘭、胡寶忠。2003。柴胡屬 (Bupleurum L.) 植物研究進展。東北農業大學學報34(3):352-359。 林俊清。2002。台灣原生藥用植物-高氏柴胡之種原培育及生物活性開發研究。農業生物技術國家型科技計劃93年度成果發表會。農業生物技術國家型科技計畫辦公室。台北。 林俊清。2003。勇抗肝炎得高氏柴胡。科學發展364:38-41。 林俊清、顏銘宏。1999。高氏柴胡的資源開發與藥效評估。藥用植物資源之開發與利用學術研討會論文集。林俊義、盧煌勝、劉新裕、陳忠川、張成國 主編 pp.51-55。臺灣省農業試驗所編印。臺中。 祖 寧、李 平。2005。柴胡皂苷的生理作用及臨床意義。中國中醫藥信息雜誌12(4):94-96。 倪力軍、鄔科芳、史萬忠、張立國。2007。解熱抗炎中藥揮發性成分的藥效學研究。中成藥 29(8):1217-1221。 柯 勇。2002。植物生理學。藝軒出版社。pp. 669-676。 陳威臣、闕甫心、夏奇鈮、葉茂生、蔡新聲。2005。2,4-D對高氏柴胡葉片癒合組織誘導、增殖與柴胡皂苷含量之影響。作物、環境與生物資訊2:39-49。 陳嘉吉、陳世賢。1995。植物種原保育政策及方向。pp.150-156。作物種原保育技術研習會專刊。溫英杰編。臺灣省農業試驗所。台中。 廖松淵。1987。大豆水分代謝、氣孔特性及組織構造之生理生態與解剖學研究。國立中興大學植物研究所博士論文。 張永文。1996。柴胡果膠多糖的結構與藥理活性研究進展。國外醫學中藥中醫分册18(4):20-23。 黃秋蘭、郭能成。1995。三島柴胡育種指標性狀之篩選與利用。臺灣地區藥用植物資源之開發與利用學術研討會專刊。杜金池、盧煌勝、劉新裕編pp.69-84。臺灣省農業試驗所。臺中。 單宇、逢煦、董云發、袁昌齊。2004。柴胡屬植物化學成分及藥理研究新進展。中國野生植物資源 23(4):5-14。 楊忠祐。2002。台農69號品種甘藷之超低溫冷凍保存研究。國立中興大學植物學研究所碩士論文。 楊秀雅。2005。高氏柴胡之組織培養條件對跟生長與柴胡皂素含量的影響。國立中興大學生命科學研究所碩士論文。 葉曉平、宋純清。柴胡皂苷藥理研究進展。2004。中草藥35(12):1434-1436。 劉新裕、王昭月、劉慧瑛、宋麗梅。1995。不同海拔與採收期對本省柴胡性狀、產量及成分影響。中華農業研究 44(3):258-278。 劉新裕、林俊儀、張成國主編 。2002。保肝藥用植物柴胡之開發與利用。出自:2002年藥用植物專輯。行政院農業委員會農業試驗所特刊第98號 pp.129-133。 劉新裕、李興進。2004。柴胡。出自:台灣農家要覽 農作篇(一)。潘芝、葉惠美、朱德民、王慶一、朱鈞、林明仁、張金城 主編pp.233-236。 劉新裕、李進興。2005。柴胡之開發利用。出自:2005藥用植物資源之開發與利用。劉新裕、林俊義、林宜信、謝伯舟 主編。行政院農業委員會農業試驗所特刊第112號 pp.87-89。 魯湘鄂、郭海福、汪洪武、李湘。2006。HPLC法測定柴胡中柴胡皂苷a,d的含量。肇慶學院學報27:43-46。 顏銘宏。1991。台灣產生藥高氏柴胡的品質評價基礎研究。高雄醫學院藥學研究所博士論文。 羅文陽。2007。番木瓜玻璃質化法超低溫冷凍保存流程之探討。國立中興大學生命科學研究所碩士論文。 Al-Ababneh S. S., Karam N. S.and Shibli R. A. 2002.Cryopreservation of sour orange (Citrus aurantium L.) by encapsulation–dehydration. In Vitro Cell. Dev. Biol. Plant 38:602-607. Ahuja S., Mandal B. B., Dixit S.and Srivastava P. S. 2002. Molecular, phenotypic and biosynthetic stability in Dioscorea floribunda plants derived from cryopreserved shoot tips. Plant Sci. 163:971-977. Bartolomé A. P., Rupérez P. and Fúster C. 1995. Pineapple fruit: morphological characteristics, chemical composition and sensory analysis of Red Spanish and Smooth Cayenne cultivars. Food Chem. 53:75-79. Bhargava S. 2006.Genetically modified cyanobacterium Nostoc muscorum overproducing proline in response to salinity and osmotic stresses. J. Biosci. 31:265-272. Bradford M. 1976. A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254. Buitink J., Hemminga M. A. and Hoekstra F. A. 1999. Characterization of molecular mobility in seed tissues: an electron paramagnetic resonance spin probe study, Biophys. J. 76:3315-3322. Buitink J. and Leprince O. 2004. Glass formation in plant anhydrobiotes: survival in the dry state. Cryobiology 48:215-228. Charoensub R., Phansiri S., Sakai A. and Yongmanitchai W. 1999. Cryopreservation of cassava in vitro-grown shoot tips cooled to -196℃ by vitrification. CryoLett. 20:89-94. Cho E. G., Hor Y. L., Kim H. H., Rao V. R. and Engelmann F. 2001. Cryopreservation of Citrus madurensis zygotic embryo axes by vitrification: importance of pregrowth and preculture conditions. CryoLett. 22:391-396. Chu C., Dai Z., Maurice S.B., and Edward G.. E. 1990. Induction of crassulacean acid metabolism in the facultative halophyte Mesembryanthemum crystallium by abscisic acid. Plant Physiol. 93:1253-1260. Crowe J. H., Crowe L. M., Carpenter J. F. and Wistrom C. A. 1987. Stabilization of dry phospholipid bilayers and proteins by sugars. Biochem J. 242:1-10. Decruse S. W., Seeni S. and Nair G. M. 2004. Preparative procedures and culture medium affect the success of cryostorage of Holostemma annulare shoot tips. Plant Cell Tissue Organ Cult. 76:179-182. Dhindsa R.S. 1991. Drought stress, enzymes of glutathione metabolism, oxidation injury, and protein synthesis in Tortula ruralis. Plant Physiol. 95:648-651. Ding F., Jin S., Hong N., Zhong Y., Cao Q., Yi G., Wang G. 2008. Vitrifcation-cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from on vitro adult shoot tips. Plant Cell Rep. 27:241-250. Dussert S., Chabrillange N., Engelmann F., Anthony F. and Hamon S. 1997. Cryopreservation of coffee (Coffea arabica L.) seeds: importance of the precooling temperature. CryoLett. 18:269-276. Ellis R. E., Hong T. and Roberts E. H. 1990. An intermediate category of seed storage behaviour ? I. coffee. J. Exp. Bot. 41:1167-1174. Engelmann F. 1997. In vitro conservation methods. In: Callow J. A., Ford-Lloyd B.V., Newbury H. J. eds. Biotechnology and Plant Genetic Resources. CAB International, Oxford, pp.119-161. Engelmann F. 2004. Plant cryopreservation: progress and prospects.In Vitro Cell. Dev. Biol. Plant 40:427-433. Fahy G. M., MacFarlane D. R., Angell C. A. and Meryman H. T. 1984. Vitrification as an approach to cryopreservation. Cryobiology 21:407-426. Frankel O. and Soule M. 1981. Conservation and evolution. Cambridge Univ. Press, Cambridge. Franks F., Hatley R. H. M. and Mathias S. 1991. Materials science and the production of shelf-stable biologicals. BioPharm. 4:38-42, 55. Franks J. R. 1999. In situ conservation of plant genetic resources for food and agriculture: a UK perspective. Land Use Policy 16:81-91. Grospietsch, M.; Stodulkova, E. and Zameenik, J. 1999. Effect of somatic stress on the dehydration tolerance and cryopreservation of Solanum tuberosum shoot tips. CryoLett. 20: 339-346. Hao Y. I. and Deng X. X. 2002. Occurrence of chromosomal variations and plant regeneration from long-term-cultured citrus callus, In Vitro Cell. Dev. Biol. Plant 38: 472-476. Helliot B, Panis B, Poumay Y, Swenen R., Lepoivre P. and Frison E.2002. Cryopreservation for the elimination of cucumber mosaicand banana streak viruses from banana (Musa spp.). Plant Cell Rep. 20:1117-1122. Helliot B., Swennen R., Poumay Y., Frison E., Lepoivre P. and Panis B. 2003. Ultrastructural changes associated with cryopreservation of banana (Musa spp.) highly proliferating meristems. Plant Cell Rep 21:690-698. Hirai D. and Sakai A. 1999. Cryopreservation of in vitro-grown axillary shoot tip meristems of mint (Mentha spicata L.) by encapsulation– vitrification. Plant Cell Rep. 19:150-155. Hirano T., Godo T., Mii M. and Ishikawa K. 2005. Cryoreservation of immature seeds of Bletilla by vitrification. Plant Physiol. 138:24-38. Hiraoka N. 1989. Bulpeurum falcatum L. embryogenesis and the production of saikosapins. p. 69-81. In: Biotechnology in Agriculture and Forestry 7. YPS Bajaj (ed.)Springer-Verlag, Berlin. Hitmi A., Sallanon H. and Barthomeuf C. 1997. Cryopreservation of Chrysanthemum cinerariaefolium Vis. cells and its impact on their pyrethrin biosynthesis ability. Plant Cell Rep. 17: 60-64. Hitmi A., Coudret A., Barthomeuf C. and Sallanon H. 1999. The role of sucrose in freezing tolerance in Chrysanthemum cinerariaefolium L. cell cultures. CryoLett. 20:45-54. Jensen W. A. 1962a. Carbohydrates and Cell Wall Constituents. In Botanical Histochemistry. pp. 198-199. Jensen W.A. 1962b. Histological Procedures. In Botanical Histochemistry Freeman and Company, San Francisco London. pp. 55-99. Jitsuyama Y, Suzuki T, Harada T. and Fujikawa S. 2002. Sucrose incubation increases freezing tolerance of asparagus (Asparagus officinalis L.) embryogenic cell suspensions. CryoLett. 23:103-112. Kan W. S. 1985. Bupleurum falcatum L., Bupleurumkaoi Liu, Chao et Chuang. (in Chinese) p.650-651. In: Manual of Medicinal Plants in Taiwan. National Research Institute of Chinese Medicine, Taipei, Taiwan. Kartha K. K. and Engelmann F. 1994. Cryopreservation and germplasm storage. In: Vasil I. K., Thorpe T. A. eds., Plant Cell and Tissue Culture. Dordrecht: Kluwer pp. 195-230. Koster K. L., Lei Y. P., Anderson M., Martin S. and Bryant G.. 2000. Effects of vitrified and nonvitrified sugars on phosphatidylcholine fluid-to-gel phase transitions. Biophysical J.78:1932-1946. Lambardi M., Fabbri A. and Caccavale A. 2000. Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips. Plant Cell Rep. 19: 213-218. Leunufna S. and Keller E. R. J. 2005. Cryopreservation of yams using vitrification modified by including droplet method: effects of cold acclimation and sucrose. CryoLett. 26:93-102. Mandal B. B., Chandel K. P. S. and Dwivedi S. 1996. Cryopreservation of yam (Dioscorea spp.) shoot apices by encapsulation–dehydration. CryoLett. 17:165-174. Matsumoto T., Sakai S. and Yamada K. 1994. Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration. Plant Cell Rep. 13: 442-446. Maxted N. 2003. Conserving the genetic resources of crop wild relatives in European Protected Areas. Biol. Conserv. 113:411-417. Maxted N., Ford-Lloyd B. V. and Hawkes J. G. 1997. Complementary conservation strategies. In: Maxted N., Ford-Lloyd B.V., Hawkes J.G. (Eds.) Plant Genetic Conservation. Chapman and Hall, London, pp. 15-39. Mazur P. 1984. Freezing of living cells: mechanisms and applications. Am. J. Physiol. Cell Physiol. 247:C125-C142. Meryman H. T., Williams R. J. and Douglas M. S. J. 1977. Freezing injury from solution effects and its prevention by natural or artificial cryoprotection. Cryobiology 14:287-302. Moges A. D., Shible R. A. and Karam N. S. 2004. Cryopreservation of African violet (Saintpaulia Ionantha Wendl.) shoot tips. In Vitro Cell. Dev. Biol. Plant 40:389-395. Niino T, Sakai A, Yakuwa H and Nojiri K. 1992. Cryopreservation of in vitro-grown shoot tips of apple and pear by vitrification. Plant Cell Tissue Organ. Cult. 28:261-266. Niino T., Tanaka D., Ichikawa S., Takano J., Ivette S., Shirata K. and Uemura M. 2003. Cryopreservation of in vitro-grown apical shoot tips of strawberry by vitrification. Plant Biotechnol. 20:75-80. Niino T., Tanaka D., Tantely R. R. and Fukui K. 2007. Cryopreservation of basal stem buds of in vitro-grown Mat Rush (Juncus Spp.) by vitrification. CryoLett. 28:197-206. Panis B., Totte N., Van K., Withers L. A. and Swennen R. 1996. Cryopreservation of banana (Musa spp.) meristem cultures after preculture on sucrose. Plant Sci. 121: 95-106. Panis B., Piette B. and Swennen R. 2005. Droplet virtification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Sci.168: 45-55. Park I. S., Kamg E. M. and Kim N. 2000. High-Performance Liquid Chromatographic analysis of saponin compounds in Bupleurum falcatum. J. Chromatogr. Sci. 38:229-233. Paulet F., Engelmann F. and Glaszmann J. 1993.Cryopreservation of apices of in vitro plantlets of sugar cane (Saccharum sp. hybrids) using encapsulation/dehydration. Plant Cell Rep. 12:525-529. Pennycooke J. C. and Towill L. E. 2000.Cryopreservation of shoot tips from in vitro plants of sweet potato [Ipomoea batatas (L.) Lam.] by vitrification. Plant Cell Rep.19: 733-737. Plessis P., Leddet C., Collas A. and Dereuddre J. 1993. Cryopreservation of Vitis vinifera L. cv. Chardonnay shoot tips by encapsulation– dehydration: effects of pretreatment, cooling and post culture conditions. CryoLett. 14:309-320. Reed, B. M. 2001. Implementing cryogenic storage of clonally propagated plants. CryoLett. 22: 97-104. Reed B. M. and Chang Y. 1997. Medium- and long-term storage of in vitro cultures of temperate fruit and nut crops. In: Razdan M. K., Cocking E. C. eds., Conservation of Plant Genetic Resources in vitro, vol. 1. General aspects. Enfield: Science Publishers pp.67-105. Reed B. M., Kovalchuk I., Kushnarenko S., Meier-Dinkel A., Schoenweiss K., Pluta S., Straczynska K. and Benson E. E. 2004. Evaluation of critical points in technology transfer of cryopreservation protocols to international plant conservation laboratories. CryoLett. 25:341-352. Reed B. M., Schumacher L., Dumet D., Benson E. E. 2005. Evaluation of a modified encapsulation–dehydration procedure incorporating sucrose pretreatments for the cryopreservation of ribes germplasm. In Vitro Cell. Dev. Biol. Plant 41:431-436. Ryynanen L. and Haggman H. 1999. Substitution of ammonium ions during cold hardening and post-thaw cultivation enhances recovery of cryopreserved shoot tips of Betula pendula. J. Plant Physiol. 154:735-742. Sae Shimizu-Sato and Hitoshi M. 2001. Control of Outgrowth and Dormancy in Axillary Buds. Plant Physiol. 127:1405-1413. Sakai A. 2004. Plant cryopreservation. In Fuller B., Lane N., Benson E. E. eds., Life in the Frozen State. FL/CRC, Boca Raton, Press. pp 329-345. Sakai A., Kobayashi S. and Oiyama I. 1990. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Rep. 9:30-33. Sakai A., Kobayashi S. and Oiyama I. 1991. Survival by vitri Wcation of nucellar cells of navel orange (Citrus sinensis var. brasiliensis Tanaka) cooled to -196℃. J. Plant Physiol. 137:465-470. Sakai A., Matsumoto T., Hirai D. and Charoensub R. 2002. Survival of tropical apices cooled to -196℃ by vitrification. In: Li P. H., Palva E. T., eds. Plant Cold Hardiness, Gene Regulation and Genetic Engineering. New York: Kluwer Academic/Plenum Publishers pp. 109-119. Sakai A., Matsumoto T., Hirai D. and Niino T. 2000. Newly developeden capsulation–dehydration protocol for plant cryopreservation. CryoLett. 21:53–62. Sarkar D. and Naik P. S. 1998. Cryopreservation of shoot tips of tetraploid Solanum tuberosum L. clones by vitrification. Ann. Bot. 82:455-462. Shibli, R. A., Haagenson, D. M., Cunningham S. M., Berg W. K. and Volence J. J. 2001. Cryopreservation of alfalfa (Medicago sativa L.) cells by encapsulation–dehydration. Plant Cell Rep. 20:445-450. Slavik B. 1974. Water in cells and tissues. In B Slavik ed. Methods of Studying Plant Water Relations. Academid Publishing House of the Czevhoslovak Academy of Science Prague, New York .pp.1-20. Suzuki T., Kaneko M., Harada T. 1997. Increase in freezing resistance of excised shoot tip of Asparagus officinalis L. by preculture on sugar-rich media. Cryobiology 34: 264-275. Taiz L. and Zeiger E. 2006. Plant Physiology-4nd ed. pp.679-680. Takagi H., Thinh N. T., Islam O. M., Senboku T. and Sakai A. 1997. Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedures. Plant Cell Rep. 16:594-599. Turner S. R., Senaratna T., Bunn E., Tan B., Dixon K. W. and Touchell D. H. 2001. Cryopreservation of shoot tips from six endangered Australian species using a modified vitrification protocol. Ann. Bot. 87:371-378. Turner S., Senaratna T., Touchell D., Bunn E., Dixon K. and Tan B. 2001. Stereochemical arrangement of hydroxyl groups in sugar and polyalcohol molecules as an important factor in effective cryopreservation. Plant Sci. 160:489-497. Urbanova M., Kosuth J. and Cellarova E. 2006. Genetic and biochemical analysis of Hypericum perforatum L. plants regenerated after cryopreservation. Plant Cell Rep.25:140-147. Vidal ., Sa´nchez C., Jorquera L., Ballester A., and Vietez A. M. 2005. Cryoreservation of chestnut by vitrification of in vitro-growth shoot tips. In Vitro Cell. Dev. Biol. Plant 41:63-68. Volk G. M. and Walters C. 2006. Plant vitrification solution 2 lowers water content and alters freezing behavior in shoot tips during cryoprotection. Cryobiology 52:48-61. Volk G. M., Harris J. L. and Rotindo K. E. 2006. Survival of mint shoot tips after exposure to cryoprotectant solution components. Cryobiology 52:305-308. Wang Q.C., Batuman O., Li P., Bar-Joseph M. and Gafny R. 2002a. A simple and efficient cryopreservation of in vitro-grown shoot tips of ‘Troyer’ citrange [Poncirus trifoliata Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation–vitrification. Euphytica 128:135-142. Wang Q.C., Batuman Ö., Li P., Bar-Joseph M. and Gafny R. 2002b. Cryopreservation of in vitro-grown shoot tips of ‘Troyer’ citrange [Poncirus trifoliata (L.) Raf. × Citrus sinensis (L.) Osbeck.] by encapsulation-dehydration. Plant Cell Rep. 20:901-906. Wang Q. C, Mawassi M, Sahar N, Li P, Violeta C-T, Gafny R, Sela I, Tanne E. and Perl A. 2004. Cryopreservation of grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation–vitrification. Plant Cell, Tissue Org. Cult. 77:267-275. Wang Q. and Laamanen J. 2005. Cryopreservation of vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification. Plant Cell Rep.24:280-288. Wang Q., Gafny R., Sahar N., Sela I., Mawassi M., Tanne E. and Perl A. 2002. Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions by encapsulation-dehydration and subsequent plant regeneration. Plant Sci. 162: 551-558. Wowk B., Leitl E., Rasch C. M., Mesbah-karimi N., Harris S. B. and Fahy G. M. 2000. Vitrification enhancement by synthetic ice blocking agents. Cryobiology 40:228-236. Yoshida S., Forno J. H., Cook H. and Gornez K. A. 1976. Determination of chlorophyll in plant tissue. Laboratory manual of physiological studies for rice. IRRI 26:43-49.
摘要: 
This study investigates the cryopreservation of the in-vitro grown axillary buds of Taiwan native medicinal herb Bupleurum kaoi Liu, Chao et Chuang(B. kaoi)using the vitrification procedure. Factors influencing the success of B. kaoi cryopreservation include the duration of exposure to the cryoprotectant solution and the composition of the post-cryostorage recovery medium. The plantlets obtained from 45-day shoot cultures were precultured for 3 days on solidified Murashige and Skoog (MS) medium supplemented with 0.2 M sucrose. The 2–3 mm excised stem segments with 1-2 axillary buds were treated for 60 min at room temperature with a loading solution (LS, 2 M glycerol + 0.4 M sucrose) and dehydrated with PVS2 (plant vitrification solution 2) for 120 min at 0℃ before rapid immersion in liquid nitrogen (LN). After 1 week in liquid nitrogen, rapid rewarming at 40℃ for 30 sec in water bath was followed by unloading in 1.2 M enriched sucrose solution for 35 min. The axillary buds were plated on petri dishes containing 1/2 MS medium as recovery medium for 2 weeks and transfered to conical flask containing 1/2 MS medium supplemented with 0.1 mg/L benzyladenine (BA) and 0.1 mg/L α-naphthaleneactic acid (NAA) for 2 month. This protocol achieved 59.63% buds recovery rates and 73.33% regeneration rate. The morphology of the plantlets after cryopreservation were indistinguishable to the normal ones. Analysis the contents of saikosaponins found that the main accumulation was in the aboveground. After cryopreservation, the subculture derived from aboveground and root of regeneration plantlet were the same as the non-cryopreserved plantlet. In conclusion, the protocol established in this study could be adapted to the cryopreservation of B. kaoi by vitrification.
URI: http://hdl.handle.net/11455/22725
其他識別: U0005-2108200812530600
Appears in Collections:生命科學系所

Show full item record
 
TAIR Related Article

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.