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Analysis of chicken testis regeneration
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觀察自體移植睪丸生長的情形，結果顯示自體移植睪丸的重量增加倍率明顯比正常發育的睪丸多。在經過12週的生長之後，可以從自體移植睪丸中得到具有活動力的精子。由此可以認為自體移植的睪丸有進行再生，並且回復原本的產精功能。進一步比較正常發育睪丸與自體移植睪丸的各細胞型態生長情形，我們利用Sox9標記發育中Sertoli cell 和Leydig cell；以及WT1 和Aromatase分別標定成熟的Sertoli cell 和Leydig cell 來探討各細胞型態在睪丸再生過程中增生與分化的狀態。在5,6週時可以開始發現有生殖細胞在增生，第7-9週體細胞(Leydig 和Sertoli cell)進行大量增生，進入第十週後體細胞的增生開始減緩，而生殖細胞則開始進行減數分裂，到了12週開始有精細胞的出現。在成功再生的自體移植睪丸內，也可以觀察到有與正常發育睪丸類似的細胞型態分佈。
Regeneration is a process that reactivates developmental programs in post-embryonic life to restore missing tissues. Chicken testis are known to regenerate in a small population of castrated males, however no detailed documentation of this unique phenomenon has being reported. We developed an auto-transplantation system that by inserting a small piece of testicular tissue under the skin can allow regeneration to occur. By continuous monitoring the development of transplanted testicular, we found the transplanted testicular tissue grow much faster than the endogenous un-castrated testes and the growth rate continues till after week 30. The histological analysis revealed slightly distorted seminiferous tubules, but the major cell types: Sertoli cells, different stages of developing sperm and Spermatogonia, and the interstitial Leydig cells are all present; furthermore we are able to collect motile sperm from this auto-grafted testicular tissue, indicating the functionality of seminiferous tubules are not damaged. According these results, we conclude that the transplanted chicken testicular tissues have regenerative ability.
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