Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23211
標題: 內皮細胞在不同粒徑奈米銀-聚胺酯複合材料上之行為評估
Behavior of endothelial cells on the nanocomposites of polyurethane and silver nanoparticles of different sizes
作者: 陳培陽
Chen, Pei-Yang
關鍵字: nanosilver;奈米銀;nanosilver-polyurethane composites;NO production;Gene expression;奈米銀-聚胺酯複合材料;NO量;基因表現
出版社: 生命科學院碩士在職專班
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摘要: 
本研究是以不同粒徑的奈米銀-聚胺酯複合材料做為研究對象,針對材料特性、材料與細胞培養、材料與細胞基因表現等三大主題的評估。首先就材料特性方面,可發現奈米銀與聚胺酯所形成的複合材料,其表面有明顯的奈米相。在細胞貼附與生長方面,發現不同粒徑的奈米銀-聚胺酯複合材料較控制組有良好的細胞貼附與生長,由SEM觀察可發現細胞在奈米銀粒徑為25 nm的奈米銀-聚胺酯複合材料貼附形態較奈米銀粒徑為45 nm的奈米銀-聚胺酯複合材料平坦且有較多的細小薄偽足(filopodia)攀附,預期可增進內皮細胞在材料上的貼附和生長。在基因表現方面;我們發現在三種不同粒徑奈米銀的奈米銀-聚胺酯複合材料,同時比較25 ppm、50 ppm、75 ppm三種濃度的NO量、鈣離子濃度、eNOS表現,在50 ppm有最佳表現。由上述結果我們可推測在奈米銀-聚胺酯複合材料50 ppm對內皮細胞的貼附生長是最佳濃度。同時可證實內皮細胞與材料間的反應機制。最後我們可知道內皮細胞在不同粒徑的奈米銀-聚胺酯複合材料中的貼附生長以25 nm的粒徑大小最佳。

The subject of this research is different particle sizes of nanosilver-polyurethane composites and it is discussed under the three topics of (1)material characteristic, (2)material and cell culture and (3)material and gene expression to analyze the experiment.
First, in material characteristic, we can find nano-phases are formed at the surface of nanosilver-polyurethane composites. In cell adhesion and growth, different particle sizes of nanosilver-polyurethane composites show better performance than the ones in control group. Through the SEM observation, we can find that the cell morphology is smoother in 25nm particle size of nanosilver-polyurethane composites than in 45nm one and there are more filopodia attached, which is an effective alternative for endothelial cells. In gene expression, we can find when NO production、calcium concentration and eNOS expression of the three concentrations(25ppm、50ppm、75ppm)were compared at the same time, the 50ppm one has the best performance among the three different particle sizes of nanosilver-polyurethane composites. The results show that the best concentration for the growth of endothelial cells is 50ppm nanosilver-polyurethane composites; meanwhile, it can be confirmed by the mechanism of reaction between endothelial cells and materials. Finally, we can understand the 25nm particle size has the best performance among the growth of different particle sizes of nanosilver-polyurethane composites.
URI: http://hdl.handle.net/11455/23211
其他識別: U0005-2007200621525600
Appears in Collections:生命科學系所

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