Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23350
DC FieldValueLanguage
dc.contributor鄭旭辰zh_TW
dc.contributor余長澤zh_TW
dc.contributor.advisor林赫zh_TW
dc.contributor.author劉美芳zh_TW
dc.contributor.authorLiu, Meei-Fangen_US
dc.contributor.other中興大學zh_TW
dc.date2009zh_TW
dc.date.accessioned2014-06-06T07:20:07Z-
dc.date.available2014-06-06T07:20:07Z-
dc.identifierU0005-0907200816431600zh_TW
dc.identifier.citation參考文獻 Ayliffe, G. A. 1997. The progressive international spread of methicillin-resistant Staphylococcus aureus. Clin. Infect. Dis. 24:74-79 Barber M. Methicillin-resistant staphylococci. J Clin Pathol 1961; 14:385-393. Barber, M., and M. Rozwadowska-Dowzenko. 1948. Infection by penicillin-resistant staphylococci. Lancet ii:641-644. Bell JM, Turnidge JD. High prevalence of oxacillin-resistant Staphylococcus aureus isolates from hospitalized patients in Asia-Pacific and South Africa: results from SENTRY antimicrobial surveillance program, 1998–1999. Antimicrob Agents Chemother 2002; 46: 879–881. Bone, R. C. 1994. Gram-positive organisms and sepsis. Arch. Intern. Med. 154:26-34 Boyce JM, Landry M, Deetz TR, DuPont HL. Epidemiologic studies of an outbreak of nosocomial methicillin-resistant Staphylococcus aureus infections. Infect Control 1981; 2: 110–116. Boyce JM, White RL, Causey WA, Lockwood WR. Burn units as a source of methicillin-resistant Staphylococcus aureus infections. JAMA 1983; 249: 2803–2807. Boyce JM.Understanding and controlling Methicillin resistant staphylococcus aureus infection. Infect Control Hosp Epidemiol 2002;23:485-487. Chabbert YA, Baudens JG, Acar JF,et al. The natural resistance of staphylococci to methicillin and oxacillin. Rev Fr Etud Clin Biol 1965;10:495-506. Chambers HF. Methicillin-resistant in staphylococci:molecular and biochemical basis and clinical implications. Clin Microbiol Rev 1997;10:781-791. Chung M, Dickinson G, De Lencastre H, Tomasz A. International clones of methicillin-resistant Staphylococcus aureus in two hospitals in Miami, Florida. J Clin Microbiol 2004; 42: 542–547. Clinical and Laboratory Standards Institute. 2005. Performance standards for antimicrobial susceptibility testing, fifteenth informational supplement. Approved standard MS100-S15. CLSI, Wayne, Pa. Cosgrove, S. E., G. Sakoulas, E. N. Perencevich, M. J. Schwaber, A. W. Karchmer, and Y. Carmeli. 2003.Comparison of mortality associated with of methicillin-resistant and of methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin. Infect. Dis. 36:53-59. Coia, J. E.,G. J. Duckworth, D. I. Edwards, M. Farrington., et al.2006. Guidelines for the control and prevention of methicillin-resistant Staphylococcus aureus (MRSA)in healthcare facilities. J. Hosp. Infect. 635:s1-s44. Crisostomo, M. I., H. Westh, A. Tomasz, M. Chung, D. C. Oliveira, and H. de Lencastre. 2001. The evolution of methicillin resistance in Staphylococcus aureus: similarity of genetic backgrounds in historically early methicillin-susceptible and -resistant isolates and contemporary epidemic clones. Proc. Natl. Acad. Sci. USA 98:9865-9870. Chambers, H. F. 2001. The changing epidemiology of Staphylococcus aureus? Emerg. Infect. Dis. 7:178-182. Chang, S., D. M. Sievert, J. C. Hageman, M. L. Boulton, F. C. Tenover, F. P. Downes, S. Shah, J. T. Rudrik, G. R. Pupp, W. J. Brown, D. Cardo, and S. K. Fridkin. 2003. Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene. N. Engl. J. Med. 348:1342-1347. Duckworth, G. J.,J. Z. Jordens.1990. Adherence and survival properities of an an epidemic methicillin-resistant strain of Staphylococcus aureus compared wth those of methicillin-sensitive strains. J. Med. Microbiol. 32:195-200 Deurenberg RH, Vink C, Oudhuis GJ et al,.. Different clonal complexes of methicillin-resistant Staphylococcus aureus are disseminated in the Euregio Meuse-Rhine region. Antimicrob Agents Chemother 2005; 49: 4263–4271. Denis O, Deplano A, Nonhoff C et al. National surveillance of methicillin-resistant Staphylococcus aureus in Belgian hospitals indicates rapid diversification of epidemic clones. Antimicrob Agents Chemother 2004; 48: 3625–3629. de Sousa, M. A., M. I. Crisostomo, I. S. Sanches, J. S. Wu, J. Fuzhong, A. Tomasz, and H. de Lencastre. 2003. Frequent recovery of a single clonal type of multidrug-resistant Staphylococcus aureus from patients in two hospitals in Taiwan and China. J. Clin. Microbiol. 41:159-163. de Sousa, M. A., I. S. Sanches, M. L. Ferro, M. J. Vaz, Z. Saraiva, T. Tendeiro, J. Serra, and H. de Lencastre. 1998. Intercontinental spread of a multidrug-resistant methicillin-resistant Staphylococcus aureus clone. J. Clin. Microbiol. 36:2590-2596. Deurenberg R. H.,C.Vink, S. Kalenic, A. W. Friedrich,et al.,2007 The molecular evolution of methicillin-resistant Staphylococcus aureus.Clin Microbiol Infect;13:222-235. Dufour, P., Y. Gillet, M. Bes, G. Lina, F. Vandenesch, D. Floret, J. Etienne, and H. Richet. 2002. Community-acquired methicillin-resistant Staphylococcus aureus infections in France: emergence of a single clone that produces Panton-Valentine leukocidin. Clin. Infect. Dis. 35:819-824. Enright, M. C., N. P. Day, C. E. Davies, S. J. Peacock, and B. G. Spratt. 2000. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J. Clin. Microbiol. 38:1008-1015. Enright MC, Spratt BG. Multilocus sequence typing. Trends Microbiol 1999; 7: 482–487. Enright, M. C., D. A. Robinson, G. Randle, E. J. Feil, H. Grundmann, and B. G. Spratt. 2002. The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA). Proc. Natl. Acad. Sci. USA 99:7687-7692. Farr, B. M., W. R. Jarvis. 2002. Would active surveillance culture help control healthcare-related methicillin-resistant Staphylococcus aureus infection?. Infect Control Hosp Epidemiol 23:65-75. Fluit, A. C., F. J. Schmitz, and J. Verhoef. 2001. Frequency of isolation of pathogens from bloodstream, nosocomial pneumonia, skin and soft tissue, and urinary tract infections occurring in European patients. Eur. J. Clin. Microbiol. Infect. Dis. 20:188-191. Frank, A. L., J. F. Marcinak, P. D. Mangat, and P. C. Schreckenberger. 1999. Increase in community-acquired methicillin-resistant Staphylococcus aureus in children. Clin. Infect. Dis. 29:935-936. Gillet, Y., B. Issartel, P. Vanhems, J. C. Fournet, G. Lina, M. Bes, F. Vandenesch, Y. Piemont, N. Brousse, D. Floret, and J. Etienne. 2002. Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 359:753-759. Gorak, E. J., S. M. Yamada, and J. D. Brown. 1999. Community-acquired methicillin-resistant Staphylococcus aureus in hospitalized adults and children without known risk factors. Clin. Infect. Dis. 29:797-800. Grundmann, H., S. Hori, and G. Tanner. 2001. Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms.J. Clin. Microbiol. 39:4190-4192. Hiramatsu, K., H. Hanaki, T. Ino, K. Yabuta, T. Oguri, and F. C. Tenover. 1997. Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J. Antimicrob. Chemother. 40:135-136. Hussain, F. M., S. Boyle-Vavra, C. D. Bethel, and R. S. Daum. 2000. Current trends in community-acquired methicillin-resistant Staphylococcus aureus at a tertiary care pediatric facility. Pediatr. Infect. Dis. J. 19:1163-1166. Hackbarth CJ, Chambers HF. Methicillin-resistant staphylococci:genetics and mechanisms of resistance. Antimicrob. Agents Chemother. 1989;33:991-994. Hirai, Y. 1991. Survival of bacteria under dry conditions;from a viewpoint of nosocomial infection. J. Hosp. Infect. 19:191-200. Hiramatsu, K., K. Okuma, X. X. Ma, M. Yamamoto, S. Hori, and M. Kapi. 2002. New trends in Staphylococcus aureus infections: glycopeptide resistance in hospital and methicillin resistance in the community. Curr. Opin. Infect. Dis. 15:407-413. HIto, T., Y. Katayama, K. Asada, N. Mori, K. Tsutsumimoto, C. Tiensasitorn, and K. Hiramatsu. 2001. Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus.Antimicrob. Agents Chemother. 45:1323-1336. Ito T, Ma XX, Takeuchi F et al. Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase ccrC. Antimicrob Agents Chemother 2004; 48: 2637–2651. Jernigan, J. A.., M. G. Titus., D. H. Groschel., et al. 1996.Effectiveness of contact isolation during a hospital outbreak of methicillin-resistant Staphylococcus aureus. Am. J Epidemiol ,143:496-504. Jevons, M. P. 1961. "Celbenin"-resistant staphylococci. Br. Med. J. 124:124-125 Jernigan JA, Titus MG, Groschel DH, Getchell-White S, Farr BM. Effectiveness of contact isolation during a hospital outbreak of methicillin-resistant Staphylococcus aureus. Am J Epidemiol 1996; 143: 496–504. Kanerva M., Blom M., Tuominen U.,et al. 2007 Costs of an outbreak of methicillin-resistant Staphylococcus aureus infection. J Hosp Infect .66:22-8 Kayser FN. Methicillin-resistant staphylococci, 1965-1975.Lancet 1975;2:650-653. Kollef MH, Inadequate antimicrobial treatment: An important determinant of outcome for hospitalized patients, Clin Infect Dis., Volume: 31, Issue: Suppl 4 (2000), pp. S131--S138 Kluytmans, J., A. van Belkum, and H. Verbrugh. 1997. Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin. Microbiol. Rev. 10:505-520. Ko KS, Lee JY, Suh JY et al. Distribution of major genotypes among methicillin-resistant Staphylococcus aureus clones in Asian countries. J Clin Microbiol 2005; 43: 421–426. Katayama, Y., T. Ito, and K. Hiramatsu. 2000. A new class of genetic element, Staphylococcus cassette chromosome mec, encodes methicillin resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 44:1549-1555. Kerr, S., G. E. Kerr, C. A. Mackintosh, and R. R. Marples. 1990. A survey of methicillin-resistant Staphylococcus aureus affecting patients in England and Wales.J. Hosp. Infect. 16:35-48. Lacey, R. W.,K. W. Barr, V. E. Barr, T. J. Inglis.1986. Properities of methicillin-resistant Staphylococcus aureus colonizing patients in a burns unit. J. Hosp. Infect. 7:137-148. Liu, P. Y. F., Y. J. Lau, B. S. Hu, et al,.1995.Analysis of clone relationships among isolates of Shigella sonnei by different molecular typing method. . J. Clin. Microbiol. 33:1779-1783. Lowy FD. Staphylococcus aureus infections. N Engl J Med 1998; 339: 520–532. Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. J Clin Invest 2003; 111: 1265–1273. Lyon BR, Skurray R. Antimicrobial resistance of Staphylococcus aureus:genetic basis. Microbiol Rev 1987;51:88-134. Murchan S, Kaufmann ME, Deplano A et al. Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 2003; 41: 1574–1585. Moran, G. J., and J. Mount. 2003. Staphylococcus aureus resistant to vancomycin—United States, 2002. Ann. Emerg. Med. 41:148-151. Ma, X. X., T. Ito, C. Tiensasitorn, M. Jamklang, P. Chongtrakool, S. Boyle-Vavra, R. S. Daum, and K. Hiramatsu.2002 . Novel type of staphylococcal cassette chromosome mec identified in community-acquired methicillin-resistant Staphylococcus aureus strains. Antimicrob. Agents Chemother. 46:1147-1152. Mazmanian, S. K., H. Ton-That, and O. Schneewind. 2001. Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus. Mol. Microbiol. 40:1049-1057. Musser, J. M., and V. Kapur. 1992. Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination. J. Clin. Microbiol. 30:2058-2063. Marples, R. R., and E. M. Cooke. 1985. Workshop on methicillin-resistant Staphylococcus aureus held at the headquarters of the Public Health Laboratory Service on 8 January 1985.J. Hosp. Infect. 6:342-348. Oliverira , D. C.,Lencastre, H. D. 2002. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant in staphylococcus aureus. Antimicrob. Agents Chemother. 46:2155-2161. Oliveira DC, Tomasz A, de Lencastre H. Secrets of success of a human pathogen: molecular evolution of pandemic clones of methicillin-resistant Staphylococcus aureus. Lancet Infect Dis 2002; 2: 180–189. Opal, S. M., and J. Cohen. 1999. Clinical gram-positive sepsis: does it fundamentally differ from gram-negative bacterial sepsis? Crit. Care Med. 27:1608-1616. Okuma, K., K. Iwakawa, J. D. Turnidge, W. B. Grubb, J. M. Bell, F. G. O''Brien, G. W. Coombs, J. W. Pearman, F. C. Tenover, M. Kapi, C. Tiensasitorn, T. Ito, and K. Hiramatsu. 2002. Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J. Clin. Microbiol. 40:4289-4294. Oliveira, D. C., and H. de Lencastre. 2002. Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 46:2155-2161. Peacock JE, Marsik FJ, Wenzel RP. Methicillin-resistant Staphylococcus aureus: introduction and spread within a hospital. Ann Intern Med 1980; 93: 526–532. Robinson DA, Enright MC. Evolution of Staphylococcus aureus by large chromosomal replacements. J Bacteriol 2004; 186: 1060–1064. Siboni K, Poulsen L, Digman E. The dominance of Methicillin-resistant staphylococci in a country hospital. Dan Med Bull 1968;15:161-165. Sanger, F. Determination of nucleotide sequence in DNA. Science. 1981. 214:1205-10. Sa-Leao R, Sanches IS, Couto I, Alves CR, de Lencastre H. Low prevalence of methicillin-resistant strains among Staphylococcus aureus colonizing young and healthy members of the community in Portugal. Microb Drug Resist 2001; 7: 237–245. Susan, B. V. E. Ben, C. C. Wang, S. D. Robert. 2005 Successful multiresistant community –associated methicillin-resistant Staphylococcus aureus lineage from Taipei, Taiwan,that carries either the novel Staphylococcal chromosome cassette mec(SCCmec)type VT or SCCmec type IV. J. Clin. Microbiol. 43:4719-4730. Tenover FC, Arbeit R, Archer G et al. Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. J Clin Microbiol 1994; 32: 407–415. van Belkum A, van Leeuwen W, Kaufmann ME et al. Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments: a multicenter study. J Clin Microbiol 1998; 36: 1653–1659. Wu SW, de Lencastre H, Tomasz A. Recruitment of the mecA gene homologue of Staphylococcus sciuri into a resistance determinant and expression of the resistant phenotype in Staphylococcus aureus. J Bacteriol 2001; 183: 2417–2424. Wielders CL, Vriens MR, Brisse S et al. In-vivo transfer of mecA DNA to Staphylococcus aureus. Lancet 2001; 357: 1674–1675. Ward TT, Winn RE, Hartstein AI, Sewell DL. Observations relating to an inter-hospital outbreak of methicillin-resistant Staphylococcus aureus: role of antimicrobial therapy in infection control. Infect Control 1981; 2: 453–459. 國家衛生研究院(2007,3月4日)。國家衛生研究院電子報188期。國衛院全球資訊網。摘自http//www.nhri.org.tw/nhri6/enews.phpzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/23350-
dc.description.abstract摘 要 金黃色葡萄球菌(Staphylococcus aureus)是醫院最常見的致病菌,自從1961年分離出第一株抗甲氧苯青黴素金黃色葡萄球菌(methecillin resistant Staphylococcus aureus;MRSA)之後,MRSA很快的出現在各國,由於抗生素的大量使用,使MRSA成為目前院內感染最常見的菌種。本研究收集台灣地區5家醫院自2001年1月至2008年3月共107株MRSA,期望藉脈衝電場電泳基因分型法(Pulsed-field gel electrophoresis;PFGE)、多基因組序列分析(Multilocuse sequence typing;MLST)兩種方法,分析菌株的基因相關性,並進一步和其他國家比較,找出台灣的本土流行株,並比較台灣不同地域MRSA的親源相關性。脈衝電場電泳結果,經比對分析菌株親緣樹枝圖後,親緣百分比>80%即定義為同一族群(cluster),因此得到五種族群:A(80.5%)、B(89.8%)、C(80.4%)、D(88.0%)與E(87.5%),其中A群與B群全台5家醫院都存在此兩群,C、D、E群則各分布在不同醫院。本次107株MRSA經MLST分型再與資料庫比對,共87株可比對,且有6種型別,包括:ST239、ST59、ST241、ST5、ST25與ST221,最主要的是ST239與ST59,各佔59.8%與13%。從本研究發現ST239是臺灣最主要的流行株,與亞洲國家有些異同,如韓國主要的流行株是ST5與ST239,而日本主要的流行株是ST5,又中國的主要菌株是ST239,和台灣具相同的流行株,原因可能是中國和台灣經常有台商和探親來往頻繁之故。另一方面,本研究呈現MRSA在臺灣的醫院是廣泛分佈的,所以醫院對於此抗藥性菌株,除了持續的監測,完備的感控措施及完善的抗生素管制外,嚴謹的院內感染管制政策的落實,是最重要的預防院內感染(nosocomial infection)之道。zh_TW
dc.description.tableofcontents目 次 誌謝--------------------------------------------------------------- i 中文摘要----------------------------------------------------------- ii 英文摘要-------------------------------------------------------- iii 目次------------------------------------------------------------------------- iv 圖表次----------------------------------------------------------------------- v 壹、前言-------------------------------------------------------------------- 1 貳、材 料 與 方 法 一、菌株來源-------------------------------------------------------------- 6 二、藥品-------------------------------------------------------------------- 7 三、培養基----------------------------------------------------------------- 7 四、抗生素----------------------------------------------------------------- 8 五、細菌之培養與鑑定-------------------------------------------------- 8 六、最低抑菌濃度-------------------------------------------------------- 11 七、脈衝電場電泳法----------------------------------------------------- 12 八、PFGE條紋片段的比對---------------------------------------------- 14 九、DNA萃取--------------------------------------------------------------- 15 十、進行MLST前的PCR反應------------------------------------------- 15 十一、多基因定序分型-------------------------------------------------- 16 十二、mecA測定---------------------------------------------------------- 17 叁、結果 一、細菌鑑定-------------------------------------------------------------- 18 二、最低抑菌濃度-------------------------------------------------------- 18 三、PCR反應--------------------------------------------------------------- 19 四、脈衝電場電泳-------------------------------------------------------- 19 五、多基因定序分型----------------------------------------------------- 20 肆、討論-------------------------------------------------------------------- 21 伍、結論-------------------------------------------------------------------- 27 陸、參考文獻-------------------------------------------------------------- 48 柒、附錄-------------------------------------------------------------------------------- 63 圖 表 次 表一金黃色葡萄球菌對oxacillin的最低抑菌濃度結果-------- 30 表二、MLST PCR引子的序列------------------------------------------ 31 表三、MLST基因型的表現--------------------------------------- 32 表四、台灣地區醫院ST的分布--------------------------------------- 33 表五、臺灣地區PFGE型別的分佈------------------------------------ 34 圖一、金黃色葡萄球菌在血液培養基的菌落型態----------------- 35 圖二、革蘭氏染色結果-------------------------------------------------- 36 圖三、tpi、gmk與pta為引子的PCR產物--------------------------- 37 圖四、mecA為引子的PCR產物--------------------------------------- 38 圖五、MRSA的脈衝電場電泳結果------------------------------------- 39 圖六、107株MRSA的PFGE結果親緣樹枝圖------------------------ 40 圖七、中榮MRSA的PFGE結果親緣樹枝圖------------------------ 41 圖八、馬偕MRSA的PFGE結果親緣樹枝圖-------------------------- 42 圖九、桃園MRSA的PFGE結果親緣樹枝--------------------------- 43 圖十、屏東MRSA的PFGE結果親緣樹枝圖------------------------ 44 圖十一、高雄MRSA的PFGE結果親緣樹枝圖-------------------- 45 圖十二、ST59的PFGE結果親緣樹枝圖------------------------------ 46 圖十三、ST239的PFGE結果親緣樹枝圖---------------------------- 47zh_TW
dc.language.isoen_USzh_TW
dc.publisher生命科學院碩士在職專班zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-0907200816431600en_US
dc.subjectMRSAen_US
dc.subject抗甲氧苯青黴素金黃色葡萄球菌zh_TW
dc.subjectPFGEen_US
dc.subjectMLSTen_US
dc.subjectclusteren_US
dc.subjectnosocomial infectionen_US
dc.subject脈衝電場電泳基因分型法zh_TW
dc.subject多基因組序列分析zh_TW
dc.subject院內感染zh_TW
dc.subject族群zh_TW
dc.title台灣地區抗甲氧苯青黴素金黃色葡萄球菌的流行病學分析zh_TW
dc.titleEpidemiology of methecillin resistant Staphylococcus aureus isolates in Taiwanen_US
dc.typeThesis and Dissertationzh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeThesis and Dissertation-
Appears in Collections:生命科學系所
Show simple item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.