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標題: Cisplatin和Go6976在乳癌細胞生長的協同抑制作用
Synergistic inhibition of breast cancer cells growth by Cisplatin and Go6976
作者: 邢華冑
Hsing, Hua-Chou
關鍵字: 順鉑;breast cancer;抗藥性;蛋白激酶抑制劑;協同作用;cisplatin;Go6973;drug resistance;protein kinase C α;inhibitor;synergistic effect
出版社: 生命科學院碩士在職專班
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Cisplatin是已知有效抗腫瘤藥劑之一,在臨床上廣泛運用在許多不同固體瘤的治療,它可以造成細胞DNA損傷,影響細胞存活甚至造成不可逆的凋亡程式,但以cisplatin為基礎的化學治療其療效受限於抗藥性的產生。已知蛋白激酶Cα與癌細胞的惡化轉型、生長、凋亡、轉移和活化有關連,研究報告顯示蛋白激酶Cα在癌症治療上是一個 具有潛力的標的蛋白。而Go6976是蛋白激酶Cα/β和檢查點激酶Chk1/Chk2的抑制劑,它會增進腫瘤細胞對毒性藥劑的敏感度。本研究使用兩種不同表現型的乳癌細胞株,MCF-7 (ER+, wild type p53) 和 MDA-MB-231 (ER-, p53 mutant),分別以cisplatin和Go6976組合處理以觀察對此兩種細胞株的細胞生長是否有抑制情形,結果顯示這兩種藥物的組合在MDA-MB-231細胞株產生明顯的協同抑制效果,而在MCF-7細胞株則無,這種不同作用機制的藥物協同作用,將可降低化療使用的藥物濃度,避免傷害正常細胞,減低藥物引起的副作用。
其他識別: U0005-1508200823274300
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