Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23378
標題: 利用聚胺酯-奈米金複合物及血管內皮細胞做為靜脈留置導管表面處理之研究
Surface modification of indwelling venous catheter by polyurethane-nanogold composite and endothelial cells coating
作者: 林昱君
Lin, Yugerne
關鍵字: tissue engineering;織工程;nanotechonology;nanogold;polyurethane;endothelial cell;indwelling venous catheter;biomaterial;rat jugular vein;air plasma;platelet adhesion test;monocyte activation test;奈米科技;奈米金;聚胺酯;血管內皮細胞;靜脈留置導管;生醫材料;大鼠頸靜脈;大氣電漿;血小板貼附試驗;單核球活化試驗
出版社: 生命科學院碩士在職專班
引用: 參考文獻 1. R.Langer and J.P.Vacanti ,Tissue engineering.Science, 1993, page 920-926. 2.財團法人器官捐贈移植登錄中心 , http://www.torsc.org.tw 3. www.apligraf.com 4. Nicolas L’Heureux, Ph.D. ,Todd N. McAllister, Ph.D.,The New England journal of medicine ,2007 ,page 1451-1453. 5. Global information Inc. , 2007. 6. University of Toronto Medical Journal, volume 81, number 3, May 2004 7.楊志明,徐善慧,組織工程,九州圖書文物有限公司 8. Petr Klement, Ying Jun Du, Leslie R. Berry …etc, Chronic performance of polyurethane catheters covalently coated with ATH complex: A rabbit jugular vein model, Biomaterials 27 (2006) 5107–5117 9. Toshihiko Shirotaa, Hisataka Yasui, Hiroaki Shimokawa…etc, Fabrication of endothelial progenitor cell (EPC)-seeded intravascular stent devices and in vitro endothelialization on hybrid vascular tissue, Biomaterials Volume 24, Issue 13, June 2003, Pages 2295-2302 10. E . McFadden , E . Stabile , E . Regar...etc, Late thrombosis in drug-eluting coronary stents after discontinuation of antiplatelet therapy, The Lancet , Volume 364 , Issue 9444 , Pages 1519 – 1521 11. Helen M. Burt, and William L. Hunter, Drug-eluting stents: A multidisciplinary success story,Advanced Drug Delivery Reviews Volume 58, Issue 3, 3 June 2006, Pages 350-357 12. M. S. Ramakrishna , D. D. Deshpande, G. N. Babu, Radiation-induced copolymerization of hydrophilic monomers: Synthesis and thermal properties, Journal of Polymer Science Part A: Polymer Chemistry Volume 26 Issue 2, , Mar 2003 Pages 445 – 455 13.林宏道,大氣電將改質聚氨酯表面應用於人工小血管材料之研究,國立中興大學化學工程研究所碩士論文,中華民國九十六年七月。 14.Marina A. Dobrovolskaia,Barry W. Neun, analysis of platelet aggregation ,Nanotechnology characterization laboratory ,National cancer institute at Frederick ,November 2005. 15. Priyabrata Mukherjee,Resham Bhattacharya,Ping Wang…etc., Antiangiogenic Properties of Gold Nanoparticles, Clin Cancer Res 2005;11(9) May 1, 2005 ,page 3530-3534 16. Hanako Kobayashi, P Charles Lin, Nanotechnology for antiangiogenic cancer therapy, Nanomedicine ,June 2006, Vol. 1, No. 1, Pages 17-22 17. C. Abdelghani-Jacquin , A. Abdelghani , G. Chmel, Decorated surfaces by biofunctionalized gold beads :application to cell adhesion studies, Eur Biophys J (2002) 31:page 102–110 18. Alison P. McGuigan and Michael V. Sefton, The influence of biomaterials on endothelial cell thrombogenicity, Biomaterials. 2007 June ; 28(16): 2547–2571 19. 周志謂,Study of NanoBiomaterials for wound dressing,中興大學博士論文。
摘要: 
摘要
靜脈留置導管在醫療上的用途很廣泛,譬如靜脈輸液、血液透析、血壓或靜脈壓力監測、化學治療…等等。但是隨之而來的,就是靜脈留置導管所引起的併發症,例如靜脈血栓、狹窄、感染和出血…等等。
造成靜脈留置導管併發症最可能的原因,就是生醫材料的血液相容性不佳和外來物反應。近年來組織工程和奈米科技的研究,發現可以增加材料的生物相容性,減少傳統生醫材料的缺點。
本實驗利用聚胺酯-奈米金複合物、大氣電漿和血管內皮細胞做為靜脈留置導管表面改質,觀察材料表面處理的不同,對於血小板貼附、單核球活化、和大鼠靜脈植入之後的反應。本實驗採用商品化靜脈留置導管(Terumo® Surflash) ,依照不同的表面處理分為下列五組:(1)控制組(2)大氣電漿(3)大氣電漿加上血管內皮細胞包覆(4)大氣電漿加上聚氨酯-奈米金複合物(5) 大氣電漿加上聚氨酯-奈米金複合物加上血管內皮細胞包覆。經由研究可以發現:(1)大氣電漿表面改質,有助於血管內皮細胞貼附在聚胺酯(靜脈留置導管的材料)的表面,但是同時也會增加血小板貼附和強烈地活化單核球,在大鼠靜脈植入模式,則有靜脈纖維化、阻塞的不佳表現。(2)聚氨酯-奈米金複合物的表面處理,同樣地有助於血管內皮細胞貼附在聚胺酯材料表面,卻不會引起強烈的血小板貼附、和單核球活化。在大鼠靜脈植入模式,有輕微的靜脈纖維化和阻塞的現象。(3)聚胺酯-奈米金複合物加上血管內皮細胞包覆的組別,結果相似於聚氨酯-奈米金複合物表面處理的效果。
基於以上的結果,希望能應用奈米科技和組織工程在人工血管、血管內支架、人工心臟瓣膜、和體外循環管路…等等方面,減少這些和血液相接觸的醫療裝置血液相容性不佳的問題,減少血栓的併發症,製造出更理想的生醫材料。

Abstract
Indwelling venous catheters are widely used in clinical medical practice, such as intra-venous fluid administration , hemodialysis , intra-vascular pressure monitor , and chemotherapy… etc. The global annual cost for indwelling venous catheters is about 800 million USD . There also many complication resulting from indwelling venous catheters, such as vein thrombosis, stenosis and infection …etc. In the recently reports ,we found that the nano-technology and tissue engineering can provide better hemo-compatibility and bio-compatibility for biomaterials of catheters .The better hemo-compatibility can further reduce thrombosis ,that seems good for indwelling venous catheter ( In this experiment ,we chose commercialized polyurethane catheter “ Terumo® surflo “) .
We designed the catheters which surface are modified by polyurethane-nanogold composite, air plasma and endothelial cells.
According to the different treatment of polyurethane catheters ,there are 5 groups(1)control group (2) air plasma treated (3) air plasma treated and endothelial cells coating (4) polyurethane-nanogold composite coating (5) polyurethane-nanogold composite and endothelial cells coating .
Rat jugular vein is used for indwelling venous catheter implantation test in animal model . Pre-implantation test are platelet adhesion test and monocyte activation test .
Result: (1) In air plasma treated group, there are more endothelial cells adhesion on air plasma treated polyurethane indwelling catheter than control group . Platelet adhesion test showed increased in number of platelet adhesion on air treated catheter than control group .Air plasma and endothelial cells coating had less vein fibrosis in animal experiment . (2) In polyurethane-nanogold composite group , there are also increasing endothelial cells adhesion effect . Platelet adhesion are fewer than air plasma treated group .Monocyte activation are low in degree. Rat jugular vein injury was slightly .(3)In polyurethane-nanogold composite and endothelial cells coating group , the result are similar to polyurethane-nanogold composite group.
Based on the above preliminary result ,we hope he polyurethane-nanogold composite can applied to the lining of artificial vascular graft, intra-vascular stent , artifical valve ,or lining of cardiopulmonary bypass circuit . If we can improve the hemo-compatibility of biomaterials , the problem of thrombo-embolism can be reduced .
URI: http://hdl.handle.net/11455/23378
其他識別: U0005-2208200814170200
Appears in Collections:生命科學系所

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