Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23511
標題: 腎葉山螞蝗及菘藍藥用植物增殖系統之建立
Establishment of multiplication protocol on medicinal plants Desmodium renifolium (L.)Schindler and Isatis indigotica Fortune
作者: 彭怡靜
Peng, I-Ching
關鍵字: Desmodium renifolium (L.)Schindler;腎葉山螞蝗;Isatis indigotica Fortune;菘藍
出版社: 生命科學院碩士在職專班
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摘要: 
植物體內所含的藥用成份極具價值,但由於很多人為因素,近年來有許多藥用植物正在消失。本試驗利用微體繁殖方法,建立腎葉山螞蝗和菘藍兩種藥用植物的增殖系統,過程包含建立無菌培植體、芽體誘導、發根及健化移植。芽體誘導試驗腎葉山螞蝗以生長調節劑BA/NAA=10/1的比例,濃度分別為BA 0.2 mg/L加NAA 0.02 mg/L、BA 0.4 mg/L加NAA 0.04 mg/L、BA 0.6 mg/L加NAA 0.06 mg/L、BA 0.8 mg/L加NAA 0.08 mg/L及BA 1 mg/L加NAA 0.1 mg/L的WPM培養基作為試驗組;未添加任何生長調節劑的WPM培養基作為對照組,結果顯示腎葉山螞蝗腋芽培養於WPM培養基添加BA 0.6 mg/L和NAA 0.06 mg/L,可獲得最多芽數,但添加生長調節劑BA會抑制植物生長,植株高度較矮小;發根試驗腎葉山螞蝗以WPM、1/2 WPM、1/2 WPM加IBA 0.2 mg/L、1/2 WPM加IBA 0.5 mg/L進行試驗,結果顯示WPM培養基是最適合的發根培養基,培養八週發根率為93.3%。芽體誘導試驗菘藍以生長調節劑BA/NAA=10/1的比例,濃度分別為BA 0.2 mg/L加NAA 0.02 mg/L、BA 0.4 mg/L加NAA 0.04 mg/L、BA 0.6 mg/L加NAA 0.06 mg/L、BA 0.8 mg/L加NAA 0.08 mg/L、BA 1 mg/L加NAA 0.1 mg/L及BA 1.4 mg/L加NAA 0.14 mg/L的MS培養基作為試驗組;未添加任何生長調節劑的MS培養基作為對照組,結果顯示菘藍腋芽培養於MS培養基添加BA 0.8 mg/L和NAA 0.08 mg/L,可獲得最多芽數;發根試驗菘藍以MS、1/2 MS、1/2 MS加IBA 0.2 mg/L、1/2 MS加IBA 0.5 mg/L、1/2 MS加NAA 0.2 mg/L、1/2 MS加NAA 0.5 mg/L進行試驗,結果顯示以1/2 MS加NAA 0.5 mg/L在第六週時發根率最佳達到100%。

The medicinal components of plants are valueable.Because of many artificial factors,there are many medicinal plants vanishing in recent years. The aims of this study were to establish in vitro multiplication protocol of Desmodium renifolium (L.)Schindler and Isatis indigotica Fortune.The processes include establishing aseptic explants,inducing shoots,rooting and transplanting.In the inducing shoots test, the experimental concentrations of Desmodium renifolium (L.)Schindler were BA 0.2 mg/L+NAA 0.02 mg/L,BA 0.4 mg/L+NAA 0.04 mg/L,BA 0.6 mg/L+NAA 0.06 mg/L,BA 0.8 mg/L+NAA 0.08 mg/L, BA 1 mg/L+NAA 0.1 mg/L with BA-NAA ratio 10:1 and the Woody plant medium (WPM) was the control group.In vitro micropropagation of Desmodium renifolium (L.)Schindler was established to get maximum shoots multiplication rate by cultivating axillary buds on Woody plant medium (WPM) supplemented with BA 0.6 mg/L+ NAA 0.06 mg/L,but added plant growth regulators suppressing growth of explants.In the rooting test, the experimental concentrations of Desmodium renifolium (L.) Schindler were WPM,1/2 WPM,1/2 WPM with IBA 0.2 mg/L and 1/2 WPM with IBA 0.5 mg/L.Rooting rate achieved 93.3% by cultivating on Woody plant medium for 8 weeks. In the inducing shoots test, the experimental concentrations of Isatis indigotica Fortune were BA 0.2 mg/L+NAA 0.02 mg/L,BA 0.4 mg/L+NAA 0.04 mg/L,BA 0.6 mg/L+NAA 0.06 mg/L,BA 0.8 mg/L+NAA 0.08 mg/L,BA 1 mg/L+NAA 0.1 mg/L,BA 1.4 mg/L+NAA 0.14 mg/L with BA-NAA ratio 10:1 and the Murashige and Skoog (MS) medium was the control group.In vitro micropropagation of Isatis indigotica Fortune was established to get maximum shoots multiplication rate by cultivating axillary buds on Murashige and Skoog (MS) medium supplemented with BA 0.8 mg/L+ NAA 0.08 mg/L. In the rooting test, the experimental concentrations of Isatis indigotica Fortune were MS,1/2 MS,1/2 MS with IBA 0.2 mg/L,1/2 MS with IBA 0.5 mg/L,1/2 MS with NAA 0.2 mg/L and 1/2 MS with NAA 0.5 mg/L.Rooting rate achieved 100% by cultivating on 1/2 MS with NAA 0.5 mg/L for 6 weeks.
URI: http://hdl.handle.net/11455/23511
其他識別: U0005-2007201013395400
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