Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23513
標題: 青脆枝及銀杏藥用植物增殖系統之建立
Establishment of multiplication protocol on medicinal plants of Nothapodytes nimmoniana (Graham) Mablerley and Ginkgo biloba L.
作者: 朱俐蒔
Ju, Li-Shr
關鍵字: 青脆枝;Nothapodytes nimmoniana (Graham) Mablerley;銀杏;Ginkgo biloba L.
出版社: 生命科學院碩士在職專班
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摘要: 
本實驗利用微體繁殖方法建立兩種藥用植物之增殖系統,為青脆枝和銀杏兩種木本植物,方法過程包含建立無菌培植體、芽體誘導、快速增殖與發根。青脆枝以BA/IBA為0/0 (對照組) 、1/0.1、2/0.2、3/0.3、4/0.4、5/0.5進行芽體增殖試驗,結果顯示青脆枝腋芽培養於BA 2 mg/L及IBA 0.2 mg/L之WPM培養基,可獲得最多芽數,若BA濃度達3 mg/L以上,植物外觀產生明顯的變異,植株矮小且葉子捲曲,第8週,BA 3mg/L其變異率為44%,BA 4 mg/L其變異率為63%,BA 5 mg/L其變異率為74%,可見濃度越高植株變異率越高。發根方面取培養於BA 1 mg/L、IBA 0.1 mg/L之WPM培養基4週的植株,再移至巨量元素減半之WPM培養基,培養8週後,可得發根率56%,顯著高於取培養於WPM培養基4週的植株再移至巨量元素減半之WPM培養基及IBA 0.5 mg/L、巨量元素減半之WPM培養基,其發根率為32%,表示發根需要BA協同作用。銀杏以BA/IBA為0/0 (對照組) 、1/0.1、2/0.2、3/0.3、4/0.4、5/0.5進行芽體增殖試驗,但銀杏有嚴重褐化問題,實驗結果表示褐化與不同植株有關係,與光照強度和BA濃度無關。為避免其褐化,改以添加5%(w/v)蔗糖、2 g/L活性碳、0.2 g/L檸檬酸之WPM培養基(新WPM)。BA 5 mg/L及IBA 0.5 mg/L可獲得最多芽數,但高濃度BA繼代易產生變異,因此以WPM為增殖培養基;培養於NAA 0.5 mg/L、巨量元素減半之新WPM培養基,8週後,可獲得最佳發根率10%,但發根結果太低,有待後續研究。

The aims of this study were to establish in vitro multiplication protocol of Nothapodytes nimmoniana(Graham)Mablerley and Ginkgo biloba L.. The process contains sterile culture, shoots induction, rapid proliferation and roots induction. Shoots multiplication of Nothapodytes nimmoniana(Graham)Mablerley takes BA/IBA 0/0 (control group), 1/0.1, 2/0.2, 3/0.3, 4/0.4,5/0.5 (five experimental groups) to proceed experiment. In vitro micropropagation of Nothapodytes nimmoniana(Graham)Mablerley was established to get maximum shoot multiplication rate by cultivating axillaries buds on WPM medium supplemented with BA 2 mg/L and IBA 0.2 mg/L. BA concentration up to more than 3 mg/L, the plant had a notable variations in appearance, small plants and leaf curl. In the eighth week, BA 3mg/L its variation rate is 44%;BA 4 mg/L its variation rate is 63%;BA 5 mg/L its variation rate is 74%, and the density higher explants variation rate is higher. Move the explants of Nothapodytes nimmoniana(Graham)Mablerley on WPM medium supplemented with BA 1 mg/L、IBA 0.1 mg/L for four weeks to WPM medium with half strength of macro elements. After eight weeks, rooting rate achieved 56% significantly higher than for explants of Nothapodytes nimmoniana(Graham)Mablerley (32%) cultivated on WPM medium for four weeks and then moved to two different mediums: WPM medium with half strength of macro elements and WPM medium with half strength of macro elements supplemented with IBA 0.5 mg/L. Represents the root induction needs BA synergies. Shoot multiplication of Ginkgo biloba L. takes BA/IBA 0/0 (control group), 1/0.1, 2/0.2, 3/0.3, 4/0.4,5/0.5 (five experimental groups) to proceed experiment. The Ginkgo biloba L. has serious problems with browning, the experimental result expressed browning has the relations with the different adult plants, has nothing to do with the strength of illumination and the BA density. In order to avoid browning, change 3% (w/v) sucrose to 5%(w/v) sucrose, 2 g/L activated carbons, 0.2 g/L citric acid in WPM medium (new WPM medium).In vitro micropropagation of Ginkgo biloba L. was establishment to get maximum shoot multiplication rate by cultivating axillaries buds on new WPM medium supplemented with BA 5 mg/L and IBA 0.5 mg/L. But high BA concentration easy to have the variation following the generation, therefore take new WPM medium. After eight weeks, rooting rate achieved 10% by cultivating on new WPM with half strength of macro elements supplemented with NAA 0.5 mg/L. The rooting rate is too low. To waits for further studying.
URI: http://hdl.handle.net/11455/23513
其他識別: U0005-2007201014195900
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