Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23585
標題: 青花菜和臺灣蝴蝶蘭花粉乾燥法超低溫冷凍保存流程之探討
Investigation on Drying Protocols of Cryopreservation of Pollen of Brassica oleracea var. italica and Phalaenopsis aphrodite subsp. formosana
作者: 邱智聖
Chiu, Chih-Sheng
關鍵字: Brassica oleracea var. italica;青花菜;Phalaenopsis aphrodite subsp. formosana;pollen;cryopreservation;臺灣蝴蝶蘭;花粉;超低溫冷凍保存
出版社: 生命科學系所
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摘要: 
花粉含水率對花粉冷凍保存存活率是一項重要的因素,本試驗利用乾燥法,分別探討青花菜(Brassica oleracea var. italica)花粉和臺灣蝴蝶蘭(Phalaenopsis aphrodite subsp. formosana)花粉塊進行超低溫冷凍保存之處理流程。結果獲知,天氣晴朗早上,採集新鮮青花菜的花苞,將花藥取出後放在室內使其自然開裂,再經矽膠乾燥脫水處理,花粉含水率為17.8%,冷凍保存後,花粉萌發率為17.7%。另一結果獲知,早上採集當天開的台灣蝴蝶蘭花朵,取出花粉塊後,以矽膠乾脫水處理,花粉塊含水率為28.0%,冷凍保存後,花粉塊萌發率為91.7%。以上青花菜花粉與臺灣蝴蝶蘭花粉塊冷凍保存之處理方法簡單、可靠,業者容易應用於產業上來進行大量配子種質保存,加速育種工作。

Using the pollen of Brassica oleracea var. italica and the pollinia of Phalaenopsis aphrodite subsp. formosana to investigate the drying protocols of cryopreservation in this study. Water content is important to pollen viability after cryopreservation. This study indicated that collecting buds of Brassica oleracea var. italica on sunny days and removing the anthers indoor placement to be dehiscing, then dring the anthers by silica gel. The water content of pollen was 17.8% before rapid immersion in liquid nitrogen. The result shows the pollen germination rate is 17.7%. Another study indicated that collecting flowers of Phalaenopsis aphrodite subsp. formosana and removing the pollinia, then drying the pollinia by silica gel. The water content of pollinia was 28.0% before rapid immersion in liquid nitrogen. The result shows the pollinia germination rate is 91.7%. The purpose of this study is easy to preserve the pollen of Brassica oleracea var. italica and Phalaenopsis aphrodite subsp. formosana for industrial application.
URI: http://hdl.handle.net/11455/23585
其他識別: U0005-0208201123421800
Appears in Collections:生命科學系所

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