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dc.contributorShuh-Ji Kaoen_US
dc.contributorHwey-Lian Hsiehen_US
dc.contributor.advisorHsing-Juh Linen_US
dc.contributor.authorHuang, Xiao-Fenen_US
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dc.description.abstract脫硝(denitrification)與厭氧氨氧化作用(anammox)是水域生態系統中主要的氮移除途徑,可減少沿岸水域之氮源儲存量,進而控制優養化。本研究以打鳥埤人工濕地為例,藉由同位素配對技術(isotope pairing technique, IPT)測量人工濕地中序列淨水單元總體氮移除速率。結果顯示,打鳥埤人工濕地系統中厭氧氨氧化作用鮮少,主要以硝化─脫硝作用為氮移除途徑,且以沉積物為氮匯場所。脫硝作用速率約為0.58~30.42 μM N2 hr-1,季節性變化明顯,以秋季高於夏、冬季;其速率主要與有機物質含量、底棲生物擾動程度、氮鹽可利用性、入流水流量及水滯留時間等因素有關。脫硝作用速率在淨水單元之間略有前期淨水單元高於後期淨水單元的趨勢。由控制實驗結果發現,打鳥埤人工濕地系統脫硝作用受有機物質含量與硝酸鹽濃度調控;脫硝作用速率隨有機物質含量及硝酸鹽濃度增加而攀升,在C/ N比為5時有最佳脫硝作用速率。打鳥埤人工濕地氮移除能力明顯高於其他水域生態系統,顯示大漢溪人工濕地的建置可有效削減生活汙水中的活性氮,進而減緩沿岸水域優養化帶來的衝擊,達到維護沿岸生態系結構與功能之目的。zh_TW
dc.description.abstractDenitrification and anammox are the dominant nitrogen loss pathways in aquatic ecosystems, which could control eutrophication by reducing nitrogen pollutants in the water. The aim of our study is to measure total nitrogen removal rates by using isotope pairing technique in all of treatment cells of the Daniaopi Constructed Wetland. The results show that anammox occurred little in the Daniaopi Constructed Wetland, whereas nitrification-denitrification was the major nitrogen removal pathway, in which sediment was the dominant sink of reactive nitrogen. Denitrification rates ranged from 0.58~30.42 μM N2 hr-1, and they were highest in autumn. Denitrification rates of the inlet cell were slightly higher than the outlet cell. Nitrogen removal rates among seasons and treatment cells were different, which were contributed by dissolved oxygen, organic matter content, benthic invertebrates burrowing, nitrogen availability and hydrologic characters. In additional, denitrification rates were controlled by organic matter content and nitrate concentration, achieving a maximum rate at C/ N ratio was 5. The Danaiopi Constructed Wetland possesses much more effective nitrogen removal capacity than other aquatic ecosystems. This suggests that the development of constructed wetlands nearby Dahan river can protect the healthy of riverine ecosystems by reducing excess bio-available nitrogen content of domestic wastewater.en_US
dc.description.tableofcontents中文摘要 i 英文摘要 ii 目次 iii 表目次 vi 圖目次 vii 第一章、緒論 1 1. 失衡的氮循環 1 2. 水域生態系統的氮移除機制 1 2.1. 脫硝作用 1 2.2. 厭氧氨氧化作用 2 2.3. 氮移除的重要性 3 3. 人工濕地的氮移除機制 3 3.1. 人工濕地歷史與其功能 4 3.2. 氮移除機制 4 4. 本研究定位與重要性 5 第二章、目的 9 1. 研究動機 9 2. 研究目的 9 第三章、材料方法 10 1. 研究地點 10 1.1. 人工濕地介紹 10 1.2. 淨水單元 10 1.3. 實驗樣點 10 2. 採樣方法 11 2.1. 採樣時間 11 2.2. 鑽心取樣 11 2.3. 水樣採集 11 2.4. 環境因子量測 11 3. 15N同位素培養實驗 11 3.1. 補充水製備 11 3.2. 樣品前處理 11 3.2.1.前置作業 11 3.2.2.樣品分裝 12 3.2.3.置換氦氣 12 3.2.4.培養管保存 12 3.2.5.預培養實驗 12 3.3. 15N同位素培養實驗 12 3.3.1.同位素配對技術 12 3.3.2.脫硝與厭氧氨氧化作用 13實驗設計 13操作流程 13實驗設計 13操作流程 14 3.3.4.有機碳源對脫硝及厭氧氨氧化作用之影響 14實驗設計 14操作流程 14 3.4. 培養管保存 14 3.5. 分析方法 14 4. 脫硝與厭氧氨氧化作用速率計算方法 15 4.1. 厭氧氨氧化作用速率計算 15 4.2. 脫硝作用速率計算 15 5. 樣品分析 16 5.1. 營養鹽濃度 16 5.2. 水中顆粒性有機碳含量 16 5.3. 水中溶解性有機碳含量 17 5.4. 沉積物有機物質含量 17 5.5. 沉積物孔隙度、密度 17 6. 統計分析 18 7. 氮移除模式 18 8. 質量平衡模式 18 第四章、結果 27 1. 環境因子 27 2. 營養鹽 27 3. 有機物質 27 4. 厭氧氨氧化作用 28 5. 脫硝作用 28 5.1. 脫硝作用潛能速率 28 5.2. 「現地」脫硝作用 28 5.3.環境因子與脫硝作用的關係 29 6. 有機碳源對脫硝作用的影響 29 7. 硝酸鹽濃度對脫硝作用潛能之影響 29 8. 氮移除模式 30 9. 質量平衡模式 30 第五章、討論 53 1. 厭氧氨氧化作用 53 1.1. 厭氧氨氧化作用不見了? 53 1.2. 影響厭氧氨氧化作用的因素 53 2. 脫硝作用 54 2.1. 脫硝作用為主要的氮移除機制 54 2.2. 硝化─脫硝作用 55 2.3. 影響硝化─脫硝作用的因素 55 2.4. N2與N2O 56 3. 影響脫硝作用的因素 56 3.1. 時空變化 56 3.1.1.季節性變化 56 3.1.2.淨水單元間變化 58 3.1.3.水體與沉積物 58 3.2. 有機碳含量 58 3.3. 硝酸鹽濃度 59 4. 同位素配對技術、氮移除模式與質量平衡模式結果之比較 59 5. 水域生態系統氮移除速率之比較 60 6. 汙染削減型人工濕地經營管理之建議 60 第六章、結論 66 第七章、參考文獻 67zh_TW
dc.subjectconstructed wetlanden_US
dc.subjectpairing techniqueen_US
dc.titleDenitrification and anaerobic ammonium oxidation in the Daniaopi Constructed Wetlanden_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
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