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dc.contributorchia-hsiu changen_US
dc.contributorchun-chi laien_US
dc.contributorssu-yu Lien_US
dc.contributor.advisorChieh-Chen Huangen_US
dc.contributor.authorLee, Ang-Hsuanen_US
dc.identifier.citation1. 吳耿東,李宏台。2007。全球生質能源應用現況與未來展望,林業研究專訊,30卷3期。 2. 吳耿東,2008。認識生質能源,物理雙月刊,30卷4期。 3. 成游貴,2006。狼尾草育種與多元化利用科學發展月刊407卷,24-29頁。 4. 楊价民,1997。瘤胃生態系統與反芻動物對養分的利用。藝軒圖書出版社,台北,中華民國。 5. 韓如暘,陳美慈,閔航,趙宇華,馬曉航, 1999,嗜熱厭氧細菌Clostridium sp. EVA4菌株直接轉化纖維素產乙醇的研究,應用與環境生物學報,第5卷。 6. 吳秋芬,2008,嗜熱厭氧狼尾草分解菌之產醇特性研究,大葉大學環境工程學系碩士班,碩士論文。 7. 王馨儀,2006,以牛糞堆肥中嗜熱厭氧菌群分解纖維素產乙醇之研究,東海大學環境科學與工程研究所,碩士論文。 8. Carpita N C and McCann M. 2000. The cell wall. In Biochemistry and Molecular Biology of Plants, Rockville, MD, pp. 52–108. 9. Taiz and Zeiger. 2002. Plant Physiology. 3rd ed. 10. Akin DE, Borneman WS, Rigsby LL, Martin SA. 1993. p-Coumaryl and feruloyl arabinoxylans from plant cell walls as substrates for ruminal bacteria. Appl Environ Microbiol 59:644-647 11. Bayer EA, Kenig R, Lamed R. 1983. Adherence of Clostridium thermocellum to cellulose. J Bacteriol 156(2):818-27. 12. Bayer EA, Shimon LJ, Shoham Y, Lamed R. 1998. Cellulosomes-structure and ultrastructure. J Struct Biol 124(2-3):221-34. 13. 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dc.description.abstract近年來石油價格迅速上漲,生質能源技術的發展再一次受到重視。由於之物化特性最近似汽油,所以丁醇被視為未來最有可能替代石油的生質燃料。如今生質燃料之技術已推向第二代,乃係以纖維廢棄物做為醱酵的原料產生質能。植物細胞壁主要由纖維素、半纖維素、木質素組成複雜並與木質素鍵結強化結構,所以需要具有纖維素分解酵素之微生物來進行降解。本研究目標利用篩自牛瘤胃的纖維素分解菌株Clostridium puniceum Ru6及Clostridium xylanolyticum Ru15 與Clostridium acetobutylicum ATCC824 建立仿生式的同步醣化醱酵丁醇與氫氣之系統。實驗以狼尾草做為基質醣化,探討在醇菌培養與共培養方式下纖維素醣化醱酵之能力,並運用回應面法求得降解纖維素醱酵產丁醇與氫氣的最佳操作條件。實驗結果顯示:於狼尾草做為單一碳源醣化實驗中,以Clostridium xylanolyticum Ru15與Clostridium acetobutylicum ATCC824有最佳的纖維素降解成果,木質纖維素的最佳之降解率高達 12 %,半纖維素降解率高達 11 %。於共培養方式降解纖維素同步醱酵之結果看來,丁醇醱酵量並無非常顯著的加乘結果。因此,改變策略建立以人工合成之纖維素分解酵素複合體之菌株與 Clostridium acetobutylicum 重組同步醣化醱酵系統,分析胞外纖維素酵素活性與醣化結果,轉植株有顯著的分解狼尾草醣化之能力。zh_TW
dc.description.abstractIn our previous study, a stable rumen-mimic bacterial consortia system that could act as functional union for biohydrogen and biofuels production was constructed. The result revealed that cellulolytic Clostridial strains were predominated in the system. In this study, to enhance the efficiency for simultaneous saccharification and fermentation (SSF) by Clostridium xylanolyticum Ru15 and Clostridium puniceum Ru6 were co-cultured to develop a dual microbial SSF system. The fermentative metabolites analyzed to isolate that including acetate, butyrate, ethanol and butanol. Since C. acetobutylicum strain 824 could efficiently utilize butyrate for butanol production. However, the co-culture system were use C. acetobutylicum strain 824 with the cellulolytic Clostridial strains that could degradation lignocellulose of napiergrass 12%, and degradation hemicellulose 11%, but that was nonsignificant additive of butanol production by SSF. Finally, in order to enhance butanol production and saccharification, we were enhanc the ability of sacharification of bacteria by synthetic biology. Our bacteria of synthetic could significant additive ability to degradation and saccharification lignocellulose.en_US
dc.description.tableofcontents封面內頁 摘要 i Abstract ii 目錄 iv 表目錄 viii 圖目錄 ix 第一章 緒論 1.1 前言 1 1.2研究背景 3 1.3研究目的 4 1.4研究策略 5 第二章 文獻回顧 2.1生質能源 9 2.2生質丁醇 10 2.3木質纖維素 11 2.3.1纖維素組成與結構 13 2.3.2纖維素 13 2.3.3半纖維素 14 2.3.4木質素 15 2.4木質纖維素分解酵素 15 2.4.1纖維素分解酵素 16 2.4.2半纖維素分解酵素 17 2.4.3木質素分解酵素 17 2.4.4纖維素酵素聚合體 17 2.5梭狀芽胞桿菌(Clostridium)生產丙酮、丁醇、乙醇(ABE) 19 2.6微生物生長溫度 20 2.7氮源 22 2.8反芻動物的瘤胃 22 2.9狼尾草 24 2.10合成生物學 24 第三章 材料與方法 3.1研究材料與儀器設備 28 3.1.1菌種與質體來源 28 3.1.2培養基組成 28 3.1.3藥品及酵素 30 3.2分析方法 30 3.2.1還原醣定性與定量 30 3.2.2纖維素分解酵素胞外活性測試 32 3.2.3纖維素分組成分析 34 3.2.4氣相分析─氫氣分析 37 3.2.5液相分析─醣、醇、酸分析 37 3.3實驗設計之回應曲面法 43 3.4分子生物技術 45 3.4.1染色體DNA萃取 45 3.4.2質體DNA萃取 46 3.4.3聚合zh_TW
dc.subjectrumen bacterialen_US
dc.subjectsynthetic biologyen_US
dc.subjectClostridium xlanolytiucmzh_TW
dc.subjectClostridium puniceumzh_TW
dc.subjectClostridium acetobutylicum ATCC824zh_TW
dc.titleApplication of rumen bacterial isolates for production of biofuels from lignocelluloseen_US
dc.typeThesis and Dissertationzh_TW
item.fulltextno fulltext-
item.openairetypeThesis and Dissertation-
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