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Effect of electrical stimulation on urethral reflex and micturition during bladder distension in anesthetized rats
|關鍵字:||Glutamate;麩氨酸;urethra;pelvic nerve-to-external urethral sphincter reflex potentiation;postvoiding reflex potentiation;HoKu;electro-acupuncture;vesico- vascular reflex;尿道;骨盆神經-尿道外括約肌反射增益效應;排尿後反射增益效應;合谷穴;電針刺激;臟器血管反射||出版社:||生命科學系所||引用:||Reference Acerill A, Cotter AC, Nayak S, Matheis RJ, Shiflett SC. Blood pressure response to acupuncture in a population at risk for autonomic dysreflexia. Arch Phy Med Rehabil 81: 1393-197, 2000 Andersson S, Ericson T, Holmgren E, Lindqvist G. Electroacupuncture effect on pain threshold measured with electrical stimulation of teeth. Brain Res 63: 393-396, 1973. Andersson KE. Pharmacology of lower urinary tract smooth muscles and penile erectile tissues. Pharmacol Rev 45: 253-308, 1993. Baba H, Ji RR, Kohno T, Moore KA, Ataka T, Wakai A, Okamoto M, Woolf CJ. Removal of GABAergic inhibition facilitates polysynaptic A fiber-mediated excitatory transmission to the superficial spinal dorsal horn. Mol Cell Neurosci 24: 818-30, 2003. Ballegaard S, Jensen G, Pedersen F, and Nissen V. Acupuncture in severe, stable angina pectoris: a randomized trial. Acta Med Scand 220: 307-313, 1986. Barnes CA. Involvement of LTP in memory: are we “searching under the street light” ? Neuron 15: 751-754, 1995. Bao YX, Yu GR, Lu HH, Zhen DS, Cheng BH, and Pan CQ. Acupuncture in acute myocardial infarction. Chin Med J (Engl) 95: 824-828, 1982. Bekkers JM, Stevens CF. Presynaptic mechanism for long-term potentiation in the hippocampus. Nature 349: 156-158, 1990. Birder LA, de Groat WC. The effects of glutamate antagonists on c-fos expression induced in spinal neurons by irritation of the lower urinary tract. Brain Res 580: 115-120, 1992. Bliss TVP, Lomo T. Plasticity in a monosynaptic cortical pathway. J Physiol 207: 61, 1970. Bliss TVP, Gardner-Medwin AR. Long-lasting increases of synaptic influence in the unanesthetized hippocampus. J Physiol 216: 32-33, 1971. Bliss TVP, Lomo T. Long-lasting potentiation of synaptic transmission in the dentate area of the anesthetized rabbit following stimulation of the perforant path. J Physiol 232: 331-356, 1973. Bliss TVP and Collingridge GL. A synaptic model of memory: long-term potentiation in the hippocampus. Nature 361: 31-39, 1993. Blok BFM. Central pathways controlling micturition and urinary continence. Urol 59: 13-17, 2002. Brager DH, Capogna M, Thompson SM. Short-term synaptic plasticity, simulation of nerve terminal dynamics, and the effects of protein kinase Cactivation in rat hippocampus. J Physiol 541: 545-559, 2002. Burke RE. Synaptic efficacy and the control of neuronal inputoutput relations. Trands Neurosci 10: 42-45, 1987. Cerne R, Jiang MC, Randi æ M. Long-lasting modification in synaptic efficacy at primary afferent synapses with neurons in rat superficial spinal dorsal horn. Soc Neurosci Abstr 17: 1331, 1991. Cervero F. Sensory innervation of the viscera: peripheral basis of visceral pain. Physiol Rev 73: 95-138, 1993. Chang HY, Cheng CL, Peng CW, Chen JJ, de Groat WC. Reflexes evoked by electric simulation of afferent axons in the pudendal nerve under empty and distended bladder conditions in urethrane-anesthetized rats. J Neurosci Method 150: 80-89, 2006. Chang HY, Cheng CL, Chen JJ, de Groat WC. Roles of glutamatergic and serotonergic mechanisms in reflex control of the external urethral spincter in urethane-anesthetized female rats. Am J Physiol Regul Integr Comp Physiol 291: R224-R234, 2006. Chang HY, Cheng CL, Chen JJ, de Groat WC. Serotonergic drugs and spinal cord transactions indicate that different spinal circuits are involved in external urethral sphincter activity in rats. Am J Physiol Renal Physiol 292: F1044-F1053, 2007. Chen KJ, Chen LW, Liao JM, Chen CH, Ho YC, Cheng CL, Lin JJ, Huang PC, Lin TB. Effects of a calcineurin inhibitor, tacrolimus (FK-506), on glutamate dependent potentiation in pelvic-urethral reflex in anesthetized rats. Neurosci. 138: 69-76, 2006. Chen KJ, Peng HY, Cheng CL, Chen CH, Liao JM, Ho YC, Liou JD, Tung KC, Hsu TH, Lin TB. Acute unilateral ureter distension inhibits glutamate-dependent spinal pelvic urethra reflex potentiation via GABAergic neurotransmission in anesthetized rats. Am J Physiol Renal Physiol 292: F1007-F1015, 2007 Cheng CL, Ma CP, de Groat WC. Effects of capsaicin on micturition and associated reflexes in rats. Am J Physiol Regul Integr Comp Physiol 265: R132- R138, 1993. Cheng CL, Chai CY, de Groat WC. Detrusor-sphincter dyssynergia induced by cold stimulation of the urinary bladder of rats. Am J Physiol Regul Integr Comp Physiol 272: R1271-R1282, 1997. Chien CT, Yu HJ, Lin TB, Chen CF. Neural mechanisms of impaired micturition reflex in rats with acute partial bladder outlet obstruction. Neurosci 96: 221-230, 2000. Chuang YC, Fraser MO, Yu Y, Beckel JM, Seki S, Nakanishi Y, Yokoyama H, Chancellor MB, de Groat WC. Analysis of the afferent limb of the vesicovascular reflex using neurotoxins, resiniferatoxin and capsaicin. Am J Physiol Regul Integr Comp Physiol 281: R1302-R1310, 2001 Collingridge GL, Singer W. Excitatory amino acid receptors and synaptic plasticity. Trends Pharmacol Sci11: 290-296, 1990. Cook AJ, Woolf CJ, Wall PD, McMahon SB. Dynamic receptive field plasticity in rat spinal cord dorsal horn following C-primary afferent input. Nature 325: 151-153, 1987. Davies CH, Stanley SJ, Pozza MF, Collingridge GL. GABA autoreceptors regulate the induction of LTP. Nature 349: 609-611, 1991. Davies SN, Lester RAJ, Reymann KG, Collingridge GL. Temporally distinct pre- and postsynaptic mechanisms maintain long-term potentiation. Nature 338: 500-503, 1989. de Groat WC. Anatomy of the central neural pathways controlling the lower urinary tract. Eur Urol 34: 2-5, 1998. de Groat WC. Integrative control of the lower urinary tract: preclinical perspective. British J Pharmacol 147:S25-S40, 2006. de Groat WC, Booth AM and Yoshimura N. Neurophysiology of micturition and its modification in animal models of human disease. In: The Autonomic Nervous System, Nervous Control of The Urogenital System, ed. Maggi CA 3: 227-289, 1993. deGroat WC, Lalley PM. Reflex firing in the lumbar sympathetic outflow to activation of vesical afferent fibres. J Physiol (Lond) 37: 289-309, 1972. deGroat WC, Yoshimura N. Pharmacology of the lower urinary tract. Annu Rev Pharmacol 41: 691-721, 2001. deGroat WC, Yoshimura N. Mechanisms underlying the recovery of lower urinary tract function following spinal cord injury. Progr Brain Res152: 59-84, 2006. Douglas RM, Goddard GV, Riives M. Inhibitory modulation of longterm potentiation: evidence for a postsynaptic locus of control. Brain Res 240: 259-272, 1982. Elgersma Y, Silva AJ. Molecular mechanisms of synaptic plasticity and memory. Curr Opin Neurobiol l9: 209-213, 1999. Gustafsson B, Wigstro¨m H. Hippcampal long-lasting potentiation produced by pairing single volleys and brief conditioning tetani evoked in separated afferents. J Neurosci 6: 1575-1582, 1986. Haas HL, Rose G. Long term potentiation of excitatory synaptic transmission in the rat hippocampus: the role of inhibitory processes. J Physiol 329: 541-552, 1982. Hinman FJ. Non-neurogenic bladder (the Hinman syndrome)-15 year later. J Urol136: 769-777, 1986. Honore´ T, Davies SN, Drejer J, Fletcher EJ, Facobse P, Lodge D, Nielsen FE. Quinoxalinediones: potent, competitive non-NMDA glutamate receptor antagonists. Science 241:701-703, 1988. Izquierdo I, Medina JH. Correlation between the pharmacology of long term potentiation and the pharmacology of memory. Neurobiol Learn Mem 63: 19-32, 1995. Jiang MC, Randic´ M. Long-lasting modification in synaptic efficacy at primary afferent synapses with neurons in rat superficial spinal dorsal horn. Third IBRO word Congress Neurosci Abstr: 307, 1991. Johnston D, William S, Jaffe K, Gray R. NMDA-receptor independent long-term potentiation. Annu Rev Physiol 54: 489-505, 1992. Kakizaki H, Yoshiyama M, Roppolo JR, Booth AM, de Groat WC. Role of glutamatergic transmission in the ascending limb of the mictruition reflex pathway in the rat. J Phamacol Exp Ther 285: 22-27, 1998 Kamo I, Connon TW, Conway DA, Torimoto K, Chancellor MB, de Groat WC, Yoshimura N. The role of bladder-to-urethral reflexes in urinary continence mechanism in rats. Am J Physiol Renal Physiol 287: F434-F441, 2004. Kauer JA, Malenka RC, Nicoll RA. NMDA application potentiates synaptic transmission in the hippocampus. Nature 334: 250-252, 1988. Katz LCand Shatz CJ, Synaptic activity and the construction of cortical circuits. Science 274: 1133-1138, 1996. Kimura A, Ohsawa H, Sato A, Sato Y. Somatocardiovascular reflexes in anesthetized rats with the central nervous system intact or acutely spinalized at the cervical level. Neurosci Res 22: 297-305, 1995. Kontani H. In vivo inhibitory effects of stimulation at the central end of pelvic nerve severed from urinary bladder on urinary bladder contraction in rats. Jpn J Pharmacol l46: 411-413, 1988. Kontani H, Kawabata Y, Koshiura R. In vivo effect of gammaaminobutyric acid on the urinary bladder contraction accompanying micturition. Jpn J Pharmacol 45: 45-53, 1987. Krassioukov AV and Weaver LC. Episodic hypertension due to autonomic dysreflexia in acute and chronic spinal cord-injured rats. Am J Physiol Heart Circ Physiol 268: H2077-H2083, 1995. Lagos P, Ballejo G. Role of spinal nitric oxide synthetase-dependent processes in the initiation of micturition hypereflexia associated with cyclopho- sphamide-induced cystitis. Neurosci 125: 663-670, 2004. Langevin HM, Churchill DL, Wu J, Badger GJ, Yandow JA, Fox JR, Krag HM. Evidence of connective tissue involvement in acupuncture. FASEB J 8: 872-873, 2002. Lee YH, Creasey GH, Lim H, Song J, Song K, Kim J. Detrusor and blood pressure responses to dorsal penile nerve stimulation during hyperreflexic contration of the bladder in patients with cervical cord injury. Arch Phy Med Rehabil 83: 136-130, 2003 Li P, Pitsillides KF, Rendig SV, Pan HL, and Longhurst JC. Reversal of reflex-induced myocardial ischemia by median nerve stimulation: a feline model of electroacupuncture. Circulation 97: 1186-1194, 1998. Li P, Tjen-A-Looi S, and Longhurst JC. Rostral ventrolateral medullary opioid receptor subtypes in the inhibitory effect of electroacupuncture on reflex autonomic response in cats: a feline model of electroacupuncture. Auton Neurosci Basic Clinical 89: 38-47, 2001. Liang YC, Huang CC, Hsu KS, Takahashi T. Cannabinoid-induced presynaptic inhibition at the primary afferent trigeminal synapse of juvenile rat brainstem slices J Physiol 555: 85-96, 2004. Liao JM, Lin CF, Ting H, Chang CC, Lin YJ, Lin TB. Electroacupuncture at Hoku elicits dual effect on autonomic nervous system in anesthetized rat. Neurosci Res 32: 15-20, 2002. Liao J M, Cheng CL, Lee SD, Chen GD, Chen KJ, Yang CH, Pan SF, Chen MJ, Huang PC, Lin TB. Impaired micturition reflex caused by acute selective dorsal or ventral root(s) rhizotomy in anesthetized rats. Neurourol Urodyn 25(3): 283-9, 2006. Liao JM, Ting H, Lee SD, Yang CH, Liou TM, Peng ML, Tsai SJ, Lin CF,Lin TB. Electro-acupuncture-induced pressor and chronotropic effects in anesthetized rats. Auton Neurosci Basic & Clinical 124: 18-25, 2006. Liao JM, Huang PC, Pan SF, Chen MJ, Tung KC, Peng HY, Shih CC, Liou YM, Chen GD, Lin TB. Spinal glutamatergic NMDA-dependent pelvic nerve-to-external urethra sphincter reflex potentiation caused by amechanical stimulation in anesthetized rats. Am J Physiol Renal Physiol 292: F1791-F1801, 2007. Lin CF, Liao JM, Tsai SJ, Chiang PY, Ting H, Tang CY, Lou KL, Hsieh LC, Wang DW, Lin TB. Depressor effect on blood pressure and flow elicited by electroacupuncture in normal subjects. Auton Neurosci 107: 60-63, 2003. Lin SY, Chen GD, Liao JM, Pan SF, Chen MJ, Chen CJ, Peng HY, Ho YC, Ho YC, Huang PC, Lin JJ, Lin TB. Estrogen modulates the spinal NMDA-mediated pelvic nerve-to-urethra reflex plasticity in rats. Endocrinol147: 2956-2963, 2006. Lin TB. Dynamic pelvic-pudendal reflex plasticity mediated by glutamate in anesthetized rats.Neuropharmacol 44: 163-170, 2003. Lin TB. Tetanization-induced pelvic to pudendal reflex plasticity in anesthetized rat. Am J Physiol Renal Physiol 287: F245-F251, 2004. Lin TB and Fu TC. Effect of electroacupucture on blood pressure and adrenal nerve activity in anesthetized rats. Neurosci Lett 285: 37-30, 2000. Lin TB and Fu TC. Which afferents induce and transmit dorsal root reflex in rats? Neurosci Lett 230: 85-88, 1998. Lin TB, Fu TC, Chen CF, Lin YJ, Chien CT. Low and high frequency electroacupuncture at Hoku elicits a distinct mechanism to activate sympathetic nervous system in anesthetized rats. Neurosci Lett 237: 75-78, 1998. Liu XG, Sandkuhler J. Long-term potentaion of C-fiber-evoked potentials in the rat spinal dorsal horn is prevented by spinal N-methyl-Daspartic acid receptor blockage. Neurosci Lett 191: 43-46, 1995. Liu XG, Sandkuhler J. Characterization of long-term potentiation of C-fiber evoked potentials in spinal dorsal horn of adult rat: essential role of NK1 and NK2 receptors. J Neurophysiol 78: 1973-1982, 1997. Liu XG, Sandkuhler J. Activation of spinal N-methyl-D-aspartate or neurokinin receptors induces long-term potentiation of spinal C-fiber-evoked potentials. Neurosci 86: 1209-1216, 1998. Loaiza LA, Yamaguchi S, Ito M, Ohshima N. Electro-acupuncture stimulation to muscle afferent in anesthetized rats modulates the blood flow to the knee joint through autonomic reflex and nitric oxide. Auton Neurosci 97: 103-109, 2002. Lozier AP, Kendig JJ. Low frequency tetanus elicits long lasting potentiation of slow ventral root potential in an isolated peripheral nerve-spinal cord preparation of the neonatal rat. Soc Neurosci Abstr 19: 233, 1993. Lux G, Hagel J, Backer P, Backer G, Vogl R, Ruppin H. Domschke S, Domschke W. Acupuncture inhibite vagal gastric acid stimulation by sham feeding healthy subjects. Gut 35:1026-1029, 1994. Madison DV, Malenka RC, Nicoll RA. Mechanism underlying longterm potentiation of synaptic transmission. Annu Rev Neurosci 14: 379-397, 1991. Maggi CA. The dual sensory and efferent functions of the capsaicin- sensitive primary sensory neurons in the urinary bladder and urethra. In: The Autonomic Nervous System, Nervous Control of The Urogenital System, ed. Maggi CA. 3: 383-422, 1993. Maggi CA, Giuliani S, Giachetti, A and Meli A. The effect of MK-801 on the mictudtion reflex in anesthetized rats. Eur J Pharmacol 181: 105-109, 1990. Mallory BS, Roppolo JR and de Groat WC. Pharmacological modulation of the pontine micturition center. Brain Res 546: 310-320, 1991. Malinow R. Transmission between pairs of hippocampal slice neurons: quantal level, oscillations and LTP. Science 252: 722-724, 1991. Martin SJ, Grimwood PD, Morris RG. Synaptic plasticity and memory: an evaluation of the hypothesis. Annu Rev Neurosci 23: 649-711, 2000. Martinez JLJ, Derrick BE. Long-term potentiation and learning. Annu Rev Psychol 47: 173-203, 1996. Mary DASG. The urinary bladder and cardiovascular reflexes Int J Cardiol 23: 11-17, 1989 Matsumoto G, Hisamitsu T, and de Groat WC. Role of glutamate and NMDA receptors in the descending limb of the spinobulbospinal micturition reflex pathway of the rat. Neurosci Lett 183: 58-61, 1995. Mendell LM. Physiological properties of unmyelinated fiber projection to the spinal cord. Exp Neurol 16: 316-332, 1966. Mendell LM. Modifiablity of spinal synapses. Physiol Rev 64: 260-324, 1984. Morrison J, Birder L, Craggs M, de Groat WC, Downie J, Drake M, Fowler C, and Thor K. Neural control. In: Incontinence, eds. Abrams P, Cardozo L, Khoury S & Wein A. pp. 363-422, 2005. Mayer ML, Westbrook GL. The physiology of excitatory amino acids in the vertebrate central nervous system. Progr Neurobiol 28: 197-276, 1987. Nowak LM, Bregestovski P, Ascher P, Herbet A, Prochiantz A. Magnesium gates glutamate-activated channels in mouse central neurons. Nature 307: 262-265, 1984. Onuma H, Lu YF, Tomizawa K, Moriwaki A, Tokuda M, Hatase O, Matsui H. A calcineurin inhibitor, FK506, blocks voltage-gated calcium channel-dependent ATP in the hippocampus. Neurosci Res 30 (4): 313-9, 1998 Randić M, Jiang MC, Cerne R. Long-term potentiation and long-term depression of primary afferent neurotransmission in the rat spinal cord. J Neurosci 13: 5228-5241, 1993. Regehr WG, Delaney KR, Tank DW.The role of presynaptic calcium inshort-term enhancement at the hippocampal mossy fiber synapse. J Neurosci 14: 523-537, 1994. Richter-Levin G, Errington ML, Maegawa H, Bliss TVP. Activation of metabolic glutamate receptor is necessary for long-term potentiation in the dentate gyrus and for spatial learning. Neuropharmacol 33: 853-857, 1994. Sandkuhler J, Liu XG. Induction of long-term potentiation at spinal synapses by noxious stimulation or nerve injury. Eur J Neurosci 10:2476-2480, 1998. Sato A, Sato Y, Suzuki A. Mechanism of the reflex inhibition of micturition contractions of the urinary bladder elicited by acupuncture-like stimulation in anesthetized rats. Neurosci Res 15: 189-198, 1992. Sato ASY, Suzuki A, Uchida S. Neural mechanism of the reflex inhibition and excitation of gastric motility elicited by acupuncture-like stimulation in anesthetized rats. Neurosci Res 18: 53-62, 1993. Sato A, Schmidt RF. Somato- sympathetic reflexes: afferent fibers, central pathways, discharge characteristics. Physiol Rev 53: 916- 947, 1973. Sato A, Schmidt RF. The modulation of visceral functions by somatic afferent activity. Jpn J Physiol 37: 1-17, 1987. Sato A., Sato Y, Schmidt RF. The impact of somatosensory input on autonomic functions. Rev Physiol Biochem Pharmacol. 130: 1- 327, 1997. Schouenberg J, Sjo¨lund BH. Activity evoked by A- and C-afferent fibers in rat dorsal horn neurons and its relation to a flexion reflex. J Neurophysiol 50: 1108-1121, 1983. Svendsen F, Tjolsen A, Hole K. AMPA and NMDA receptor-dependent spinal LTP after nociciptive titanic stimulation. Neuro Report 9: 1185-1190, 1998. Sheardown MJ, Niesen EO, Hansen AJ, Jacobsen P, Honore T. 2,3-Dihydroxy-6- nitro-7-sulfamoyl-benzo(F)quinoxaline: a neuro-protectant for cerebral ischemia. Science 247, 571-574, 1990. Siegelbum SA, Kandel ER. Learning-related synaptic plasticity: LTP and LTD. Curr Opin Neurobiol 1: 113-120, 1991. Silva AJ, Paylor R, Wehner JM, Stevens CF, Tonegawa S. Imparired spatial learning in alpha-calcium-calmodulin kinase II mutant mice.Science 257: 206-211, 1992. Stener-Victorin E, Kobayashi R, Kurosawa M. Ovarian blood flow response to electro-acupuncture stimulation at different frequencies and intensities in anesthetized rats. Auton. Neurosci 108: 50-56, 2003a. Stener-Victorin E, Kobayashi R, Watanabe O, Lundeberg T, Kurosawa M. Effects of electo-acupuncture stimulation of different frequencies and intensities on ovarian blood flow in anesthetized rats with steroid-induced polycstic ovaries. Reprod Biol Endocrinol. 2: 16, 2003b. Sugaya K, de Groat WC. Effect of MK-801 and CNQX, glutamate receptor antagonists, on bladder activity in neonatal rats. Brain Res 640: 1-10, 1994. Sugiyama Y, Xue YX, Mano T. Transient increase in human muscle sympathetic nerve activity during manual acupuncture. Jpn. J. Physiol 35: 337-335, 1995. Thompson SWN, King AE, Woolf CJ. Activity-dependent changes in rat ventral horn neurons in vitro: summation of prolonged afferent evoked postsynaptic depolarizations produce a D-2-amino-5-phosphonovaleric acid sensitive windup. Eur J Neurosci 2: 638-649, 1990. Thor KB, Muhlhauser MA. Vesicoanal, urethroanal, and urethrovesical reflexes initiated by lower urinary tract irritation in the rat. Am J Physiol Regul Integr Comp Physiol 277: R1002-R1012, 1999. Ting H, Liao JM, Lin CF, Chiang PY, Chang CC, Kuo DY, Lin TB. Pressor effect on blood pressure and renal nerve activity elicited by electroacupuncture in intact and hemorrhage rats. Neurosci Lett 327: 5-8, 2002. Watkins JC and Evans RH. Excitatory amino acid transmitters. Annu Rev Pharmacol Toxicol 21: 165-204, 1981. Watkins JC, Krogsgaard-Larsen P, Honore´ T. Structureactivity relationships in the development of excitatory amino acid receptor agonists and competitive antagonists. Trends in Pharmacol Sci11: 25-33, 1990. Willete RN, Morrison S, Sapru HN, and Reis DJ. Stimulation of opiate receptors in the dorsal ponine tegmentum inhibits reflex contraction of the urinary bladder J Pharmacol Exp Ther 244: 403-409, 1988. Wingstrom H, Gustafsson B. Facilitation of hippocampal long-lasting potentiation by GABA antagonists. Acta Physiol Scand125: 159-172, 1985. Woolf CJ. Evidence for a central component of postinjury pain hypersensitivity. Nature 306: 686-688, 1983. Woolf CJ. Generation of acute pain: central mechanisms. Br Med Bull 47: 523-533, 1991. Woolf CJ, Wall PD. Relative effectiveness of C primary afferent fibers of different origins in evoking a prolonged facilitation of the flexor reflex in the rat. J Neurosci 6: 1433-1442, 1986. Yokoyama O, Yoshiyama M, Namiki M, de Groat WC. Glutamatergic and dopaminergic contributions to rat bladder hyperactivity after cerebral artery occlusion. Am J Physiol Regu lIntegr Comp Physiol 276: R935-R942, 1999. Yoshimura N. Bladder afferent pathway and spinal cord injury: possible mechanisms inducing hyperreflexia of the urinary bladder. Prog Neurobiol 57: 583-606, 1999. Yoshimura N and de Groat WC. Plasticity of Na+ channels in afferent neurones innervating rat urinary bladder following spinal cord injury. J Physiol 503: 269-276, 1997 Yoshiyama M, Roppolo JR, de Groat WC. Effects of LY215490 a comparative alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptor antagonist on the micturition reflex in the rat. J Pharmacol Exp Ther 280: 894-904, 1993a. Yoshiyama M, Roppolo JR, de Groat WC. Effects of MK-801 on the micturition reflex in the rat - possible sites of action. J Pharmacol Exp Ther 265: 844-850, 1993b. Yoshiyama M, Roppolo JR, de Groat WC. Effects of GYKI52466 and CNQX, AMPA/kainite receptor antagonist, on the micturition reflex in the rat. Brain Res 691: 185-194, 1995a. Yoshiyama M, Roppolo JR, de Groat WC. Interactions between NMDA and AMPA/kainite receptors in the control of micturition in the rat. Eur J Pharmacol 287: 73-78, 1995b. Yoshiyama M, Roppolo JR, de Groat WC. Effects of LY215490 a comparative alpha-amino-3-hydroxy-5-methyl-4-isoxazoleproprionic acid (AMPA) receptor antagonist, on the micturition reflex in the rat. J Pharmacol Exp Ther 280: 894-904, 1997. Yoshiyama M, Erickson KA, Erdman SL, de Groat WC. Effects of N-methyl-D- aspartate (dizocilpine) and alpha-amino-3-hydroxy-5-methyl-4- isoxazoleproprionate (LY215490) receptor antagoinists on the voiding reflex induced by perineal stimulation in the neonatal rat. Neurosci 90: 1415-1420, 1999. Yoshiyama M, Nezu FM, Yokoyama O, Chancellor MB, de Groat WC. Influence of glutamate receptor antagonist on micturition in rats with spinal cord injury. Exp Neurol 159: 250-257, 1999. Yoshiyama M, de Groat WC. Supraspinal and spinal alpha-amino-3-hydroxy-5- methyl-4-isoxazoleproprionic acid and N-methyl-D-aspartate glutamatergic control of the micturition reflex in the urethane-anesthetizedrat. Neurosci 132: 1017-1026, 2005.||摘要:||
骨盆神經－尿道外括約肌 (external urethral sphincter, EUS) 反射是膀胱儲存尿液的重要脊髓反射，當骨盆傳入神經持續接受低頻率的重複性電刺激，發現能引發反射活性產生長時間的增強現象，此為骨盆神經－EUS反射增益效應，於增加尿道阻力及尿液蓄積功能上具有顯著影響力。近來，合谷 (Hoku) 穴位的刺激廣泛被運用於處理自主神經功能障礙相關疾病，有實驗顯示，刺激合谷穴位可經體神經－交感神經反射改善膀胱過動的現象。本研究主要目的 (1) 探討膀胱擴張的機械刺激與規律性膀胱收縮週期 (rhythmic micturition contraction, RMCs) 對骨盆神經－EUS反射增益效應的影響，並探討興奮性神經傳導物質麩氨酸在反射增益效應中所扮演的角色；(2) 探討電刺激合谷穴位對膀胱過度擴張－引發等容積膀胱收縮 (isovolumic bladder contractions, IVCs) 與血壓的影響，同時觀察其在臨床上的應用性。因此，本實驗利用不同體積的生理鹽水，使麻醉鼠的膀胱擴張產生機械刺激，同時給予骨盆傳入神經測試性電刺激 (Test stimulation，TS，1/30 Hz，此頻率無法引發反射增益效應)，觀察骨盆神經－EUS反射活性的變化 (實驗一)；另外以連續灌流生理鹽水的方法引發膀胱產生 RMCs，觀察膀胱內壓的變化與引發骨盆神經－EUS 反射增益效應的關聯性 (實驗二)；實驗三藉由麻醉鼠的尿道導管灌注1.5 倍排尿閾值體積的生理鹽水，促使膀胱擴張並產生規律性的 IVCs，同時於合谷穴進行不同頻率的電針刺激，觀察電針刺激對IVCs 與血壓的影響。
由實驗一的結果發現，當麻醉鼠的膀胱排空 (0 cmH2O) 時，TS引發基礎反射活性；重複性刺激 (repetitive stimulation，RS，1 Hz) 則可誘發骨盆神經－EUS反射增益效應；但在膀胱被生理鹽水擴張的條件下 (6~20 cmH2O)， TS可激發骨盆神經－EUS反射活性產生增益現象，而此效應則被 APV或 NBQX (麩氨酸NMDA 或AMPA 接受器的拮抗劑) 完全或部份抑制；相反地，在膀胱排空狀態下由椎管注射麩氨酸或 NMDA可明顯增加反射活性。由上述結果得知，生理鹽水擴張膀胱的機械性刺激，可以引發骨盆神經－EUS 反射增益效應，而且此增益效應受麩氨酸機制的調控。
實驗二的結果顯示，骨盆神經－EUS反射活性隨著儲尿期 (STOR) 及排尿上升期 (VOID-asc) 膀胱內壓 (intravesical presure，IVP) 的增加而產生增益現象，隨著排尿壓力下降期 (VOID-dec) 而減少。但在排尿收縮剛結束 IVP呈現低壓時，TS仍可引發強直後增益效應 (posttentanic potentiation)，即排尿後反射增益現象 (postvoiding reflex potentiation，PVP)。將TS 誘發之反射活性與 IVP 進行回歸分析發現，PVP呈現最大斜率， STOR的斜率也顯著高於VOID-asc 或 VOID-des (P < 0.01)。CNQX (麩氨酸 AMPA 接受器的拮抗劑) 或APV分別使STOR、VOID-asc、VOID-des以及PVP的回歸線斜率顯著下降或減低到近乎水平的程度 (P < 0.01，n = 10)。由上述結果推論，在生理狀態下，可以激發骨盆神經－EUS反射產生增益效應，而且此反射活性的增加與麩氨酸機制有關。
由實驗三的數據得知，IVCs 經由臟器血管反射 (vesico-vascular reflecx，VVR) 使血壓升高；在合谷穴分別進行低頻率 (low frequency，LF，2 Hz) 或高頻率 (high frequency，HF，20 Hz) 的電針刺激 (electro-acupuncture，Ea)，則引起血壓有持續性 (tonic) 或短暫性 (phasic) 的升壓反應，在 IVCs 產生的同時給予 Ea 刺激，發現 IVCs 的振幅及頻率沒有明顯變化。由上述結果推論，本實驗室進行的電針刺激並未如預期影響膀胱本身的活性，但 Ea (electro-acupuncture) 與 VVR (vesico-vascular reflex) 兩者所引發的升壓反應有加成效應。因此，我們建議在未來臨床應用針灸處理的過程中，應更小心監控病人有無自主功能異常 (autonomic dysfunction) 的病徵或症狀。
The pelvic-to-external urethral sphincter (EUS) reflex activity plays an essential role in developing urethral resistance, particularly in the storage phase of micturition cycle. Pelvic nerve-to-EUS reflex potentiation, which may be physiologically relevant in urethra closer mechanism, is characterized by a long-lasting pelvic nerve-to-EUS reflex activity induced by repetitive stimulation (RS, 1 Hz). Recently, acupuncture of the Hoku point was widely employed to treat patients suffering from various autonomic disorders. Researchers investigating the mechanism underlying acupuncture suggested that stimulation at Hoku might ameliorate hyperactive bladder via somato-sympathetic reflexes. The purposes of our present studies are (1) to demonstrate the effect of urinary mechanical stimulation and rhythmic micturition contractions (RMCs) on pelvic nerve-to-EUS reflex potentiation and to clarify the underlying glutamatergic mechanism, (2) to evaluate the effect of electro-acupuncture (Ea) on bladder-distention induced isovolumic bladder contractions (IVCs). Therefore, we tried to find out whether the reflex potentiation would be evoked by test stimulation (TS, 1/30 Hz) on pelvic afferent nerve under two bladder distention models: (1) incremental bladder intravesical pressure (IVP, 0~20 cmH2O) of anesthetized rats was induced by infusion of fixed volume saline into the bladder (experiment I) and (2) RMCs of anesthetized rats was induced by a continuous saline infusion (experiment II). In experiment III, the effect of Ea with different stimulation frequencies on IVCs and blood pressure was studied.
In experiment I, the EUS electromyogram activity, evoked by TS or by RS, was recorded with/without bladder saline distension (DIS/non-DIS). TS +non-DIS (0 cmH2O) evoked a single action potential in the reflex activity, whereas RS + non-DIS and TS +DIS (6 20 cmH2O) both elicited a long-lasting reflex potentiation. The TS+DIS -induced reflex potentiation was abolished or attenuated by APV or NBQX (a glutamatergic NMDA or AMPA receptor antagonist). Intrathecal administration of glutamate and NMDA both induced long-lasting reflex potentiation, which was similar to the reflex potentiation observed from TS+DIS. Our findings suggested that saline distension of the rat bladder may elicit a pelvic nerve-to-EUS reflex potentiation and the glutamatergic mechanism may contribute to the presence of such a reflex potentiation.
In experiment II, through the intrathecal administration of CNQX (a glutamatergic AMPA receptor antagonist) and APV, TS-evoked reflex activities were demonstrated to potentiate as the IVP of storage phase (STOR) and of ascending period in voiding phase (VOID-asc) increased. In contrary, the TS-evoked reflex activities were reduced as the IVP of descending period in voiding phase (VOID-des) decreased. A TS-evoked postvoiding reflex potentiation (PVP), also characterized as posttetanic potentiation, was observed right after the occurrence of a voiding contraction. Regression analysis of the relationship between evoked activities and IVP revealed that the slope value of the regression line of evoked activities versus the IVP during the STOR was significantly (p<0.01) higher than that of VOID-asc or VOID-des. In addition, the slope of the regression line of PVP was significantly higher than that of the STOR (p<0.01). Intrathecal administration of CNQX or APV significantly decreased the slopes of the STOR, VOID-asc, VOID-des and PVP regression lines (p<0.01, n=10). Our results suggested that a potentiation in the pelvic nerve-to-EUS reflex could be induced under physiological conditions and the glutamatergic mechanism appeared to be involved in this reflex potentiation.
In experiment III, the pressor effects on blood pressure (BP) elicited by Ea stimulations and vesico-vascular reflex (VVR) were investigated in an over-distended bladder animal model (1.5 times volume of saline that micturition threshold hold). The Hoku (Li-4) was stimulated by a low frequency Ea (LFEa, 2 Hz) and a high frequency Ea (HFEa, 20 Hz) with intensities 20 times that of the motor threshold. The patterns of pressor effects elicited by an LFEa and an HFEa at Hoku were different, i.e., LFEa at Hoku elicited a tonic pressor effect, while HFEa elicited a phasic one. The VVR induced by IVCs also elicited a pressor effect on BP. Ea stimulation did not modify the amplitudes or frequencies of IVCs, but both of VVR- and Ea-induced pressor responses were superimposed. Our data showed that the bladder function was not significantly affected by Ea stimulation. In addition, the BP pressor effects elicited by the VVR and the Ea stimulation were additive responses. Therefore, monitoring for signs and symptoms of autonomic dysfunction is suggested to patients that received acupuncture treatments.
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