Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23865
標題: 牛樟芝純化物誘導人類血癌細胞分化之研究
Induction of differentiation by pure compounds of Antrodia cinnamomea in human acute myeloid leukemia cells
作者: 董佩綾
Tung, Pei-Ling
關鍵字: Acute myeloid leukemia;牛樟芝;Antrodia cinnamomea;cell differentiation;急性骨隨性白血病;細胞分化
出版社: 生命科學院碩士在職專班
引用: 臧穆、蘇慶華。1990。我國台灣產靈芝屬新種-樟芝。雲南植物研究 12: 395-396。 李炫璋。2003。牛樟芝菌絲體之體內保肝功能評估及其熱水萃取物在體外對基質金屬蛋白水解脢活性之影響。國立中興大學食品科學研究所碩士論文。 劉俊仁。2002。中草藥抗癌機制研究:(壹):黃芩素及黃芩苷;對血管新生作用之影響及其機制探討;(貳):樟芝活性多醣體之生物活性分析及其經由免疫調節抑制腫瘤生長之研究。國立台灣大學生物化學暨分子生物學研究所博士論文。 廖英明. 1998. 菇類中的許不了∼樟芝. 農業世界雜誌 176:76-79. 張東柱。1997。牛樟芝病害。牛樟生物學及育林技術研討會論文集。林業叢刊第72號。 陳勁初、林文鑫、陳清農、許勝傑、黃仕政、陳炎鍊。2001。台灣特有真菌-樟芝菌絲體之開發。中華真菌學會會刊。16: 7-22。 陳清農、林文鑫、盛莉莎、黃仕政、陳勁初、劉宗榮。2001。樟芝對Sprague-Dawley大鼠之連續投藥口服急性毒性。中華保健食品學會第二屆第一次會員大會。 黃惟敏。1999。樟芝微量之成分研究。靜宜大學應用化學所碩士論文。 賴宏亮、莊秀琪、呂美津、陳武元。2002。樟芝免疫調節活性之研究。保健食品研究開發與管理研討會。 楊書威。1991。中藥樟菇活性成份研究。台灣大學藥學研究所碩士論文。 黃鈴娟。2000。樟芝與姬松茸之抗氧化性質與其多醣組成分析。國立中興大學食品科學研究所碩士論文。 李順來博士,台灣國寶牛樟芝,世茂出版有限公司。2009. 行政院衛生署,2009,台灣。 呂鋒洲。2002。牛樟芝各抗腫瘤有效成份的分離、純化,誘發腫瘤凋亡及其作用機轉的研究。行政院國家科學委員會輔助專題研究計畫成果報告。 Ala-Kokko, L., Pihlajaniemi, T., Myers, J.C., Kivirikko, K.I., Savolainen, E.R. 1987. Gene expression of type I, III and IV collagens in hepatic fibrosis induced by dimethylnitrosamine in the rat. Biochem. J. 244: 75-79. Antman, K.H. 2001. Introduction: the history of arsenic trioxide in cancer therapy. Oncologist. 6. Suppl 2: 1-2. Bennet, J.M., Catovsky, D., Daniel, M.T., Flandrin, G., Galton, D.A., Gralnick, H.R. 1976. Proposals for the classification of the acute leukemia. French-American-British (FAB) co-operative group. Br. J. Haem. 33: 451-458. Bernard, J., Weil, M., Boiron, M., Jacquillat, C., Flandrin, G., Gemon, M.F. 1973. Acute promyelocytic leukemia: results of treatment by daunorubicin. Blood. 41: 489-496. Braylan, R.C., Atwater, S.K., Diamond, L., Hassett, J.M., Johnson, M., Kidd, P.G. 1997. U.S-Canadian Consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: data reporting. Cytometry. 30: 245-248. Breene, W. M. 1990. Nutritional and medical value of specialty mushroom. J. Food Protect. 53: 883-894. Breitman, T.R., Collins, S.J., Keene, B.R. 1981. Terminal differentiation of human promyelocytic leukemia cells in primary culture in response to retinoic acid. Blood. 57: 1000-1004. Breitman, T.R., Selonick, S.E., Collins, S.J. 1980. Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc. Natl. Acad. Sci. USA. 77: 2936-2940. Chang, T.T., Chou, W.N. 1995. Antrodia cinnamomea sp. Nov on Connmomum Kamehirai in Taiwan Mycology Research. 99: 756-758. Chang, P. N., J. C. Mao, S. H. Huang, L. Ning, Z. J. Wang, T. On, W. Duan & Y. Z. Zhu.2006. Analysis of cardioprotective effects using purified Salvia miltiorrhiza extract on isolated rat hearts. J. Pharmaceut. Sci. 101: 245-249. Chen, C.H., Yang, S.W., Shen, Y.C. 1995. New steroid acids from Antrodia cinnamomea, a fungal parasite of Cinnamomum micranthum. J. Natural Products. 58: 1655-1661. Chen, H.S., Tsai, Y.F., Lin, S., Lin, C.C., Khoo, K.H., Lin, C.H., Wong, C.H. 2004. Studies on the immuno-modulating and anti-tumor activities of Ganoderma lucidum (Reishi) polysaccharides. Bioorg. Medicinal Chem. 12: 5595-5601. Chen, G. Q., Shen, Z.X., Wu, F., Han, J.Y., Miao, J.M., Zhong, H.J. 1996. Pharmacokinetics and efficacy of low-dose all-trans retinoic acid in the treatment of acute promyelocytic leukemia. Leukemia. 10: 825-828. Cherng, I.H., Chiang, H.C., Cheng, M.C., Wang, Y. 1995. Three New triterpenoids from Antrodia cinnamomea. J. Natural products. 58: 365-371. Cherng, I.H., Wu, D.P., Chiang, H.C. 1996. Triterpenoids from Antrodia connamomea. Phytochem. 41: 263-267. Chiang, H.C., Wu, D., Cherng, I.W., Ueng, C.H. 1995. A sesquiterpene lactone phenyl and biphenyl compounds from Antrodia cinnamomea. Phytochem. 39: 613-616 Cho, C.S, Chung, W.S, Lee, K.W, Cheng, H.K, Yue, K.M. 2006. Ginsenoside Re of Panan ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozocin-induce diabetic rats. Eur. J. Pharmacol.550: 173-179. Dasso, M., and Newport, J.W. 1990. Completion of DNA replication is monitored by a feedback system that controls the initiation of mitosis in vitro: studies in Xenopus. Cell. 61: 811-823. Drobyski, W.R. 2004. The role of allogeneic transplantation in high-risk acute myelogenous leukemia. Leukemia. 18: 1565-1568. Faguet, G.B. 1994. Chronic lymphocytic leukemia: an updated rewiew. J. Clin. Oncology. 12: 1974-1990. Friend, C., Scher, W., Holland, J.G., Sato, T. 1971. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethylsulfoxide. Proc. Natl. Acad. Sci. USA.68: 378-382. Gao, H., Zhao, F., Chen, G. D. 2009. Bidesmoside triterpenoid glycosides from Stauntonia chinensis and relationship to anti-inflammation. J. Phytochem. 70(6): 795-806. Gerring, S. L., Spencer, F. and Hieter, P. 1990. The CHL 1 (CTF 1) gene product of Saccharomyces cerevisiae is important for chromosome transmission and normal cell cycle progression in G2/M. EMBO J. 9: 4347-4358. Harper. 2003. Harper’s Biochemistry. 26th Ed. Ch.13. pp. 514-516. Hengartner, M.O. 2000. The biochemistry of apoptosis. Nature. 407: 770-776. Honma, Y., Hozumi, M., Abe, E., Konno, K., Fukushima, M., Hata, S. 1983. 1 alpha,25-dihydroxyvitamin D3 and 1 alpha-hydroxyvitamin D3 prolong survival time of mice inoculated with myeloid leukemia cells. Proc. Natl. Acad. Sci. USA. 80: 201-204. Hseu, Y.C., Chang, W.C., Hseu, Y.T., Lee, C.Y., Yeeh, Y.J., Chen, P.C., Chen, J.Y., Yang, H.I. 2002. Protection of oxidative damage by aqueous extract from Antrodia camphorate mycelia in normal human erythrocytes. Life Sci. 71: 469-482. Hseu, Y.C., Yang, H.L., Lai, Y.C., Lin, J.G., Chen, G.W., Chang, Y.H. 2004. Induction of apoptosis by Antrodia camphorate in human premyelocytic leukemia HL-60 cells. Nutr Cancer. 48: 189-197. Hsu, Y. L., Kuo, P. L. & Lin, C. C. 2004. Proliferative inhibition, cell-cycle dysregulation, and induction of apoptosis by ursolic acid in human non-small cell lung cancer A549 cells. Life Sci. 75: 2303-2316. Huang, M.E., Ye, Y.C., Chen, S.R., Chai, J.R., Lu, J.X., Zhoa, L., Gu, L.J., Wang, Z.Y. 1988. Use of all-trans retinoic acid in the treatment of acute promyelocytic leukemia. Blood. 72: 567-572. Kantarjian, H.M., Smith, T.L., O’Brien, S., Beran, M., Pierce, S., Talpaz, M. 1995. Prolong survival in chronic myelogenous leukemia after cytogenetic response to interferon-alpha therapy. Ann Intern Med. 122: 254-261. Karawajew, L., Rhein, P., Czerwony, G., Ludwig, W.D. 2005. Stress-induced activation of the p53 tumor suppressor in leukemia cells and normal lymphocytes requires mitochondrial activity and reactive oxygen species. Blood. 105(12):4767-75. Kassi, E., Papoutsi, Z., Pratsinis, H. 2007. Ursolic acid, a naturally occurring triterpenoid, demonstrates anticancer activity on human prostate cancer cells. J. Cancer Res. Clin. Oncol. 133(7): 4935. Kerr, J.F., Wyllie, A.H., Currie, A. R. 1972.Apoptosis: a basic biological phenomenon with wide-ranging implication in tissue kinstics. Br J. Cancer. 26: 239-257. Kim, D. K., Baek, J. H., Kang, C. M., Yoo, M. A., Sung, J. W., Chung, H. Y., Kim, N. D., Choi, Y. H., Lee, S. H. & Kim, K. W. 2000. Apoptotic activity of ursolic acid may correlate with the inhibition of initiation of DNA replication. Int. J. Cancer. 87: 629-636. Krykowski, E., Polkowska-kulesza, E., Robak, T., Matusewicz, W., Urbanska-Rys, H., Holub, A. 1987. Analysis of prognostic factors in acute leukemia in adults. Haematol Blood Transfus. 30: 369-372. Lee, I.H., Huang, R.I., Chen, C.T., Chen, H.C., Hsu, W.C., Lu, M.K. 2002. Antrodia camphorate polysaccharides exhibit anti-hepatitis B virus effects. FEMS Microbiol. Lett. 209: 63-67.. Liu, J.J., Huang, T.S., Hsu, M.L., Chen, C.C., Lin, W.S., Lu, F.J., Chang, W.H. 2004. Antitumor effects of the partially purified polysaccharides from Antrodia camphorate and the mechanism of its action. Toxicol. Appl. Pharmacol.2001: 186-193. Madamanchi Geethangili and Yew-Min Tzeng . 2009. Review of Pharacological Effects of Antrodia camphorata and its Bioactive Compounds. eCAM . 2009: 1-15. McCarthy, D.M., San Migual, J.F., Freake, H.C., Green, P.M., Zola, H., Catovsky, D. 1983. 1,25-dihydroxyvitamin D3 inhibits proliferation of human promyelocytic leukemia (HL-60) cells and induces monocyte-macrophage differentiation in HL-60 and normal human bone marrow cells. Leukemia Res. 7: 51-55. Nakamura, N., Hirakawa, A., Gao, J.J., Kakuda, H., Shiro, M., Komatsu, Y., Sheu, C.C., Hattori, M. 2004. Five new maleic and succinic acid derivatives from the mycelium of Antrodia camphorate and their cytotoxic effects on LLC tumor cell line. J. Natural Products. 67: 46-48. Nowak, D., Stewart, D., Koeffler, H.P. 2009. Differentiation therapy of leukemia: 3 decades of development. Blood. 113: 3655-3665. Nurse, P. and Bissett, Y. 1981. Gene required in Gl for commitment to cell cycle and in G2 for control of mitosis in fission yeast. Nature. 292: 558-560. Place, A.E., Suh, N., Williams, C.R., Risingsong, R., Honda, T., Honda, Y., Gribble, G.W., Leesnitzer, L.M., Stimmel, J.B., Willson, T.M., Rosen, E., Sporn, M.B. 2003. The novel synthetic triterpenoid, CDDO-imidazolide, inhibits inflammatory response and tumor growth in vivo. Clin Cancer Res.Jul; 9(7): 2798-806. Sachs, L. 1978. Control of normal cell differentiation and the phenotypic reversion of malignancy in myeloid leukemia. Nature. 274: 535-539. Setoguchi, M., Nasu, N., Yoshida, S., Higuchi, Y., Akizuki, S., Yamamoto, S. 1989. Mouse and human CD14 (myeloid cell-specific leucine-rich glycoprotein) primary structure deduced from cDNA clones. Biochim. Biophys. Acta. 1008: 213-222. Shen, Y.C., Wang, Y.H., Chou, Y.C., Chen, C.F., Lin, L.C., Chang, T.T., Tien, J.H., Chou, C.J. 2004. Evaluation of the anti-inflammatory activity of zhankuic acids isolated from the fruiting bodies of Antrodia camphorate. Planta Medica. 70: 310-314. Shiao, M.S. 2003. Natural products of the medicinal fungus Ganoderma lucidum: occurrence, biological activities, and pharmacological functions. Chem Rec. 3: 172-180. Simmons, D.L., Tan, S., Tenen, D.G., Nicholson-Weller, A., Seed, B. 1989. Monocyte antigen CD14 is a phospholipid anchored membrane protein. Blood. 73: 284-289. Soignet, S.L., Frankel, S.R., Douer, D., Tallman, M.S., Kantarjian, H., Calleia, E. 2001. United States multicenter study of arsenic trioxide in relapsed acute promyelocytic leukemia. J. Clin Oncol. 19: 3852-3860. Solovjov, D.A., Pluskota, E., Plow, E.F. 2005.Distinct roles for the alpha and beta subunits in the functions of integrin alpha M beta 2. J. Biol. Chem. 280: 1336-1345. Song, T.Y., Hsu, S.I., Yeh, C.T., Yen, G.C. 2005. Mycelia from Antrodia camphorate in Submerged culture induce apoptosis of human hepatoma HepG2 cells possibly through regulation of Fas pathway. J. Agric. Food Chem. 53: 5559-5564. Song, T.Y., Yen, G.C. 2002. Antioxidant properties of Antrodia camphorate in submerged culture. J. Agric. Food Chem. 50: 3322-3327. Stone, R.M., Mayer, R.J. 1993. Treatment of the newly diagnosed adult with de novo acute myeloid leukemia. Hematol Oncol Clin North Am. 7: 47-64. Tang, W., Liu J. W., Zhao, W.M., Wei, D.Z., Zhang, J.J. 2006. Ganoderic acid T from Ganderma lucidymmycelia indeces mitrochondria mediated apoptosis on lung cancer cells. Life Sci. 80: 205-211. Testa, U., Riccioni, R. 2007. Deregulation of apoptosis in acute myeloid leukemia. Haematol. 92: 81-94. Tsai, Z.T. and Liaw, S.L. 1985. The use and the effect of Ganoderma. San Yun Press:Taichung, Taiwan. pp: 116. Wasser, S.P. 2002. Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl. Microbiol. Biotechnol. 60: 258-274. Wieland, T., Wieland, O. 1959. Chemistry and toxicology of the toxins of Amanita phalloides. Pharmacol. Rev. 11: 87-107. Wu, S.H., Ryvarden, L., Chang, T.T. 1997. Antrodia camphorate (“niu-chang-ohih”), new combination of a medicinal fungus in Taiwan. Botan. Bull. Acad. Sinica. 38: 273-275. Wu, T.S., Shi, L.S., Kuo, S.C. 2001. Cytotoxicity of Ganoderma lucidum triterpenes. J. Natural Products. 64: 1121-1122. Yang, H. L. et al. 2006. Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett. 231: 215-27. Zhu, J., Chen, Z., Lallemand-Breitenbach, V., de The H. 2002. How acute promyelocytic leukemia revived arsenic. Nat. Rev. Cancer. 2: 705-713.
摘要: 
牛樟芝(Antrodia cinnamomea)為臺灣傳統中藥,具有抗氧化及治療癌症的功效,其中包括抗血癌。血癌細胞為一群分化不完全的細胞,因此誘導血癌細胞分化,為一種急性血癌的主要治療策略,但是否牛樟芝具有促進血癌細胞分化的能力,目前並沒有相關的文獻報導。本研究主要為探討牛樟芝純化物誘導血癌細胞HL-60及KG-1分化的現象。在本實驗中,以五種不同的牛樟芝純化物(Antcin A, B, C, Zhankuic acid C及 Methyl antcinate A),處理HL-60及KG-1細胞後,觀察其生長及分化之情形。其結果顯示,HL-60及KG-1細胞在牛樟芝純化物,Antcin B及Zhankuic acid C,處理後,產生細胞數減少及存活率降低之現象,但在另外三種牛樟芝純化物(Antcin A, C, 及 Methyl antcinate A)中,並未看到明顯之現象。此外,兩株細胞皆可發現細胞分化之型態變化。利用流式細胞儀分析出單核球分化之細胞表面特異性分子CD14及CD11b表現量會增加。由實驗結果證實, Antcin A, B, C, Zhankuic acid C及 Methyl antcinate A可抑制血癌細胞HL-60及KG-1之生長及誘導其分化為單核球細胞。此結果顯示牛樟芝中所含的化學物質具有誘導血癌細胞分化及抑制血癌細胞生長的能力,而具有抗血癌之潛力。

Antrodia cinnamomea is a well known medicinal mushroom, and it has been shown to exhibit antioxidant and anticancer treatment effects. Cell differentiation is essential for normal growth and hematopoiesis. Inducing myelopoiesis can potentially serve as differentiation therapy for acute myeloid leukemia (AML). In this study, the ability of pure compounds purified from Antrodia cinnamomea to induce differentiation was studied in culture human myelocytic leukemia HL-60 and KG-1 cells. Treatment of HL-60 and KG-1 cells with Antcin B and Zhankuic acid C significanitly induced cell viability. In contrast, Antcin A, C, and Methyl antcinate A did not shown. The morphological investigation, and used the flow cytometry that also increased the levels of monocyte differentiation markers, CD14 and CD11b in both two cells. The data suggest that Antcin A, B, C, Zhankuic acid C and Methyl antcinate A exerts growth inhibition and monocyte-like differentiation on HL-60 and KG-1 cells through induction differentiation and that chemical compounds purified from Antrodia cinnamomea may have anticancer properties valuable for application in drug products.
URI: http://hdl.handle.net/11455/23865
其他識別: U0005-0802201201213700
Appears in Collections:生命科學系所

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