Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23896
標題: 紅莖椒草及沉香藥用植物增殖系統之建立
Establishment of multiplication protocols on medicinal plants of Peperomia sui and Aquilaria agallocha
作者: 高茂圍
Gau, Mau-Wei
關鍵字: Peperomia sui Lin & Lu;紅莖椒草;Aquilaria agallocha Roxb;沉香
出版社: 生命科學院碩士在職專班
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摘要: 
本試驗利用微體繁殖方法建立 2 種藥用植物之增殖系統,為紅莖椒草與沉香2 種藥用植物,方法過程包含建立無菌培植體、芽體誘導、快速增殖與發根。紅莖椒草以 BA/NAA 為 0/0 (對照組)、0.1/0.01、0.2/0.02、0.4/0.04、0.8/0.08 mg/L 添加於 MS 培養基,進行芽體增殖試驗,試驗結果顯示紅莖椒草腋芽培養會隨著 BA/NAA 濃度的提高而快速增加芽數, BA 0.1 mg/L 及 NAA 0.01 mg/L 之 MS 培養基培養 10 週後,每個培植體平均得到 6.5 個芽體。發根培養基以 MS 培養基與 1/2 MS 培養基,培養 2 週後,發根率為 100 %。沉香以 BA 0.5 mg/L 及 IBA 0.1 mg/L 加 0 g/L 活性碳 (對照組)、1、2、4、8、16 g/L 於 WPM 培養基,進行芽體增殖試驗,實驗結果顯示沉香腋芽培養於 BA 0.5 mg/L 及 IBA 0.1 mg/L 加 1 g/L 活性碳之 WPM 培養基,可獲得無變異之芽體,若沒有添加活性碳,植株葉片較小;且呈捲曲狀。發根培養基以添加 NAA 1.0 mg/L 之 1/2 WPM 培養基培養 10 週後,發根率為 46.7 %。

The aims of this study were to establish in vitro multiplication protocols of Peperomia sui Lin & Lu and Aquilaria agallocha Roxb. The processes included sterile culture, shoot induction, rapid proliferation and root induction. Shoot multiplication of Peperomia sui Lin & Lu took BA/NAA 0/0 (control group), 0.1/0.01, 0.2/0.02, 0.4/0.04 and 0.8/0.08 mg/L (four experimental groups) to proceed this experiment. In vitro micropropagation of Peperomia sui Lin & Lu was established to get the shoot multiplication rate, 6.5 shoots per explants after 10 weeks, by cultivating axillary buds on MS medium supplemented with BA 0.1 mg/L and NAA 0.01 mg/L. The rooting rate achieved 100% by cultivating on the MS medium and 1/2 MS medium after two weeks. Shoot multiplication of Aquilaria agallocha Roxb. took WPM medium supplemented with BA 0.5 mg/L and IBA 0.1 mg/L and activated charcoal 0 g/L (control group), 1, 2, 4, 8, 16 g/L (five experimental groups) to proceed the experiment. In vitro micropropagation of Aquilaria agallocha Roxb. was established to get the good multiplication rate of normal shoots by cultivating axillary buds on WPM medium supplemented with BA 0.5 mg/L and IBA 0.1 mg/L and activated charcoal 1 g/L. Without activated charcoal, the plants had a notable variation in appearance, and the leaves curled. The rooting rate achieved 46.7% by cultivating on 1/2 WPM medium with NAA 1.0 mg/L after ten weeks.
URI: http://hdl.handle.net/11455/23896
其他識別: U0005-2108201118113300
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