Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23903
標題: 內共生菌應用於香蕉之生物防治及生長促進之研究
Application of Endophytes on Biocontrol and Plant-Growth Promotion of Bananas
作者: 江幸鎂
Chiang, Hsing-Mei
關鍵字: 內共生菌;生物防治;促進植物生長;香蕉黃葉病;Burkholderia cenocepacia strain 869T2;endophyte;biocontrol;plant-growth promotion;Fusarium wilt of banana、Burkholderia cenocepacia strain 869T2
出版社: 生命科學院
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摘要: 
微生物在農業應用上早期的相關研究多以根圈菌 (rhizobacteria) 為主,但是根圈菌在施用時容易受土壤、氣候、微生物等外在環境因子影響,因此位於植物體內受到植物保護而較不易受外在環境因子影響的內共生菌之研究逐漸興起。內共生菌 (endophytes) 為生長於植物細胞間隙,但不造成植物傷害的微生物,在農業應用上有生物防治 (biocontrol)、促進植物生長 (plant-growth promotion)、植生復育 (phytoremediation) 等。香蕉黃葉病 (Fusarium wilt of banana) 為一種難以根治的土傳性病害 (soilborne disease),目前尚無有效之化學防治與生物防治方法,因而成為香蕉的世界性經濟病害,因此本研究欲藉由內共生菌之研究尋找防治之道。本研究除了解析香蕉的內共生菌族群外,同時從對香蕉黃葉病感病之北蕉及抗病之寶島蕉根部分離得到對香蕉黃葉病病原菌 (Fusarium oxysporum f. sp. cubense tropical race 4) 有氣體抑制作用及促進生長之菌株,以及另外從培地茅根部分離得到對香蕉黃葉病有抑制作用之菌株 (Burkholderia cencocepaica strain 869T2)。由拮抗結果顯示, B. cenocepacia strain 869T2 抑制香蕉黃葉病病原菌之效果大於本次研究自香蕉篩選得到之內共生菌。接種 B. cenocepacia strain 869T2 後可使北蕉之香蕉黃葉病發病率及發病程度下降,並促進北蕉之生長,使株高和葉片數都增加。由本次研究結果顯示,以殖入內共生菌作為香蕉之生物防治及促進生長之手段,深具實用潛力。

Previously, microorganisms used on biocontrol depended mostly on rhizobacteria. However, since the rhizobacteria were easily influenced by the eviromental factors, such as climate and other microorganisms…etc, the study of endophytes becomes attractive topic for researchers. Endophytes are microorganisms that live intercellular or intracellular in plants but won't harmful to plants. Endophytes can widely use on agriculture, for example biocontrol, plant-growth promotion, and phytoremediation…etc. On the other hand, Fusarium wilt of banana is a soilborne plant disease which is hard to be cured. Because there is no chemical or biological method of prevention to Fusarium wilt of banana, it turns out to be a worldwide disease. So, the aim of this research is used endophytes to find out the solution of curing Fusarium wilt of banana. In this research, we not only analyze the diversity of bananas' endophytic population but also isolated the banana endophytes which can inhibit and promote the growth of the pathogen of Fusarium wilt of banana (Fusarium oxysporum f.sp. cubense tropical race 4). And, we also isolated an endophyte designated as Burkholderia cenocepacia strain 869T2 form the root of vetivers. The inhibit ability of Burkholderia cenocepacia strain 869T2 to the pathogen of Fusarium wilt of banana is stronger than those endophytes isolated from bananas in this research. Banana (Musa sapientum) cv. Giant Cavendish inoculated the Burkholderia cenocepacia strain 869T2 can decrease the morbidity and severity of Fusarium wilt of banana and promote the growth of Banana (Musa sapientum) cv. Giant Cavendish. The results of this research showed that endophyte, Burkholderia cenocepacia strain 869T2, has good potential for biocontrol of Fusarium wilt of banana and promote the growth of bananas.
URI: http://hdl.handle.net/11455/23903
其他識別: U0005-2208201117004900
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