Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/23907
標題: 建立四氧嘧啶誘發糖尿病紐西蘭白兔眼睛相關疾病之動物模式及評估牛磺酸之保護效果
Establishment of Alloxan-induced Diabetic New Zealand White Rabbit Model and the Assessment of Taurine on the Protection of Diabetes-Related Eye Disorders
作者: 葉上民
Yeh, Shang-Min
關鍵字: Diabetes;糖尿病;Eyes;Taurine;眼睛;牛磺酸
出版社: 生命科學院碩士在職專班
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Predominant cone photoreceptor dysfunction in a hyperglycaemic model of non-proliferative diabetic retinopathy. Disease models & mechanisms 2010; 3(3-4):236-45. American Diabetes Association. Executive Summary: Standards of medical care in diabetes- 2009. Diabetes Care 2009; 32(suppl 1):s6-12. American Diabetes Association. Position statement: diabetic retinopathy. Diabetes Care 1998; 21(suppl 1):s47-49. Anand P, Rajakumar D, Felix AJ and Balasubramanian T. Effects of oral administration of antioxidant taurine on haematological parameters in Wistar rats. Pakistan journal of biological sciences : PJBS 2010; 13(16):785-93. Anastasi E, Dotta F, Tiberti C, Vecci E, Ponte E and Di Mario U. Insulin prophylaxis down-regulates islet antigen expression and isletautoimmunity in the low-dose Stz mouse model of diabetes. Autoimmunity 1999; 29(4):249-56. Appen RE, Chandra SR, Klein R, and Myers FL. Diabetic papillopathy. American journal of ophthalmology 1980; 90(2):203-9. 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摘要: 
糖尿病對眼睛影響甚鉅,而牛磺酸(Taurine)為已知的抗氧化劑,於哺乳動物視網膜具較多之牛磺酸含量,故本研究利用紐西蘭白兔建立一完整糖尿病誘發眼睛疾病之較佳動物模式,並探討牛磺酸對其之保護效用。10週齡雄性紐西蘭白兔以100 mg/kg劑量之四氧嘧啶(Alloxan)誘發第一型糖尿病,並將測試血糖連續3週達200 mg/dl之誘導成功糖尿病動物分為三組,分別為對照組(CON, n=6)、糖尿病兔給予一般飲水(DW, n=8)以及糖尿病兔之飲水中添加1%牛磺酸(DT, n=8)。於24週試驗期間,每週觀察其體重、血糖、水晶體、視網膜及眼球屈光狀態,每4週檢查其視網膜電波圖(Electroretinography, ERG),並且於第24週犧牲採集眼球組織進行組織切片評估。結果顯示DW組與DT組體重減輕、血糖增加、屈光不正增加相較於CON組具顯著差異(P<0.05),而DT組在血糖值與屈光值則介於CON組與DW組之間,且DW組之水晶體白內障發生率顯著高於DT組(P<0.05)。視網膜電圖檢查方面,DT組之A波振幅顯著高於較DW組(P<0.05)。經組織切片評估發現DW組水晶體與視網膜病變明顯較DT組增加(P<0.05)。此研究成功建立完整糖尿病誘發眼睛相關疾病之紐西蘭白兔動物模式,並顯示牛磺酸於視網膜功能具保護效果。

Diabetes is highly related to eye disorders of patients. Taurine, a conditionally essential amino acid, is capable of scavenging free radicals and exists in the retina of mammals. The objectives of this study were to establish a rabbit model of diabetes-related eye disorders and evaluate the protective effects of taurine on the diabetes-related eye disorders. Type I diabetes were induced in 10-week-old New Zealand White rabbits by a 100 mg/kg alloxan injection. Hyperglycemia (blood glucose concentration over 200 mg/dl for 3 weeks) rabbits were randomly allocated to two groups, i.e., with (DT, n=8) or without (DW, n=8) supplementation with 1% (w/v) taurine in their drinking water. Healthy rabbits without alloxan administration were served as the Control group (CON, n=6). Related parameters including body weight, blood glucose concentrations, refraction, lens and fundus examinations were measured during the 24-week trial period, and electroretinography (ERG) was recorded every 4 weeks. The histological examinations were evaluated in end of the trial.
Results showed that increased blood glucose concentration and refraction as well as decreased body weight are observed in DW and DT groups compared to the CON group (P<0.05). The percentage of cataract in DT group is lower than that in DW group (P<0.05). In addition, a functional evaluation of the retina by ERG revealed that the amplitude of a-wave in DT group is higher (P<0.05) than that in DW group which were not different (P>0.05) from that in the CON group. The abnormity of lens and retina in DW group is higher than that in DT group by histological examination. Based on our data, the diabetes-related eye disorders can be recapitulated in rabbit model and taurine shows its protective roles in diabetes-related eye disorders.
URI: http://hdl.handle.net/11455/23907
其他識別: U0005-2708201110274600
Appears in Collections:生命科學系所

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