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Formation of droplet and mixing through flow-focusing on stationary and rotating microfluidics
|關鍵字:||Droplet;微液滴;Flow-focusing;U-type channel;流體聚焦;U型流道||出版社:||機械工程學系所||引用:||Anna SL, Bontoux N, Stone HA, “Formation of dispersions using “flow focusing” in microchannels. ” Applied Physics Letters, Vol. 82, 2003, pp. 364-366. Beer NR, Hindson B, Wheeler E, Hall S, Rose K, Kennedy I, Colston B, “On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets, ” Analytical Chemistry, Vol. 79, 2007, pp. 8471-8475. Bendib S, Francais O,“Analytical study of microchannel and passive microvalve application to micropump simulator, ” In: Proceedings of the SPIE, 1999, pp. 200-208. Bringer MR, Gerdts CJ, Song H, Tice JD, Ismagilov RF, “Microfludic systems for chemical kinetics that rely on chaotic mixing in droplet,” Philosophical Transactions of the Royal Society of London.Series A:Mathematical, Physical and Engineering Sciences, Vol. 362, 2004, pp. 1087-1104. 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本研究分別以壓力及離心力驅動微流道流體，利用流體聚焦(flow-focusing)方式使消散相流體頸縮，生成由兩種液體混合的微液滴，觀察其混合效率。為了迅速增強液滴內的混合效率，流體聚焦流道下游增加製作U型流道之混合區。我們使用黃光微影製程製作母模，並利用PDMS轉印微流道結構。實驗流體以油為連續相流，水為消散相流，產生油包水(water-in-oil)的微液滴，經由可視化設備觀察液滴生成與混合現象。在壓力驅動實驗中，連續相與消散相流道寬度為100 μm，固定消散相流率為0.01 ml/hr，調整連續相流率於0.02 - 0.06 ml/hr的範圍，可產生成直徑114~135 μm的液滴。在流體聚焦處剛生成的液滴，由於頸縮作用可提供60 %的預混合效率，再搭配下游U型混合區，混合效率可迅速提升至95 %。在離心力驅動實驗中，連續相與消散相流道寬度分別為300 與200 μm，可生成微液滴的範圍為400-700 rpm。在轉速400、500及700 rpm下，可生成液滴直徑分別為402、452與900 μm，搭配U型混合區，混合效率可達80%以上。
Experiments were carried out to investigate fluid mixing within droplets generated using the flow-focusing method in microchannels. Polydimethylsiloxane (PDMS) was employed to fabricate the microchannels using the photolithography technique. The microchannels were composed of a Y-junction to bring two types of liquids into contact, a cross-junction to from droplets and a U-shaped channel to further enhance the droplet mixing. Oil as a continuous-phase flow and water as a dispersed-phase flow were injected into the channels by syringe pumps or by centrifuge on a rotating disk. In the pressure-driven microchanels that had the same width of 100 μm, the continuous-phase flow rate was varied between 0.02-0.06 ml/hr (at a constant dispersed-phase flow rate of 0.01 ml/hr) to produce droplets having diameters ranging between 114 to 135 μm. It is found that the mixing efficiency can reach as much as 60% when the droplet is just formed via flow focusing and the downstream U-shaped channel easily raises it to around 95%. In the centrifuge-driven microfluidcs that consists of a 300-μm-width continuous-phase channel and a 200-μm-width channel dispersed-phase channel, the droplets can be formed only at a certain range of rotational speed, 402-452 μm in diameter at 400-500 rpm and 900 μm in diameter at 700 rpm. It is also found that mixing efficiency can be largely increased to about 85% for the channels with U-shaped structure.
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