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標題: 台灣土雞肝臟差異表現基因GST-CL3、Otrf、FASN、INSIG1、apoVLDLⅡ及apo B基因單一核苷酸多態性之研究
Single Nucleotide Polymorphisms of GST CL3, Otrf, FASN, INSIG1, apoVLDL II , and apoB Genes Differentially Expressed in Liver of Taiwan Country Chickens
作者: 陳子翰
Chen, Tzu-Han
關鍵字: Liver;單一核苷酸多態性;SNP;Taiwan country chickens;台灣土雞
出版社: 動物科學系所
引用: Amy, C. M., B. Williams-Ahlf, J. Naggert, and S. Smith. 1992. Molecular cloning of the mammalian fatty acid synthase gene and identification of the promoter region. Biochem. J. 271:675-679. Armstrong, R. N. 1997. Structure, catalytic mechanism, and evolution of the glutathione transferases. Chem. Res. Toxicol. 10:2-18. Back, D. W., M. J. Goldman, J. E. Fisch, R. S. Ochs, and A. G. Goodridge.1986. The fatty acid synthase gene in avian liver. J. Biol. Chern. 261:4190-4197. Beckett, G. J. and J. D. Hayes. 1993. Glutathione S-transferases: biomedical applications. Adv. Clin. Chem. 30:281-380. Berkowitz, E. A. and M. I. Evans. 1992. Functional analysis of regulatory regions upstream and in the first intron of the estrogen-responsive chicken very low density apolipoprotein Ⅱ gene. J. Biol. Chem. 267:7134-7138. Bhargava, M. M., I. Listowsky, and I. M. Arias. 1978 Studies on subunit structure and evidence that ligandin is a heterodimer. J. Biol. Chem. 253:4116-4119. Binder R., C. C. McDonald, J. B. Burch, C. B. Lazier, and D. L. Williams. 1990. Expression of endogenous and transfected apolipoprotein Ⅱ and Vitellogenin Ⅱ genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4:201-208. Blue, M. L., A. A. Protter, and D. L. Williams. 1980. Biosynthesis of apolipoprotein B in rooster kidney, intestine, and liver. J. Biol. Chem. 255:10048-10051. Board P. G., R. T. Baker, G. Chelvanayagam, and L. S. Jermiin. 1997. Zeta, a novel class of glutathione transferases in a range of species from plants to humans. Biochem. J. 328:929-935. Botstein, D., R. L. White, M. Skolnick, and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:314-331. Chamberlain, J. S., R. A. Gibbs, J. E. Rainer, P. N. Nguyen, and C. T. Casey. 1988. Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res. 16:11141-11156. Chan, L. 1983. Hormonal control of apolipoprotein synthesis. Annu. Rev. Physiol. 45:615-623. Chan, L., R. L. Jackson, B. W. O' Malley, and A. R. Means. 1976. Synthesis of very low density liporpotiens in the cockerel: Effects of estrogen. J. Clin. Invest. 58:368-379. Chan, L., W. A. Bradley, A. Dugaiczyk and A. R. Means. 1980. Lipoprotein bio synthesis: The avian model. Ann. NY Acad. Sci. 348:427-428. Chang, L. H., L. F. Chuang, C. P. Tsai, C. P. D. Tu, and M. F. Tam. 1990. Characterization of glutathione S-transferases from day-old chick livers. Biochemistry 29:744-750. Chang, L. H., J. Y. Fan, L. F. Liu, S. P. Tasi, and M. F. Tam. 1992. Cloning and expression of a chick liver glutathione S-transferase CL3 subunit with the use of a baculovirus expression system. Biochem. J. 281:545-551. Chen, H., and M. R. Juchau. 1998. Recombinant human glutathione S-transferases catalyse enzymic isomerization of 13-cis-retinoic acid to all-trans-retinoic acid in vitro. Biochem. J. 336:223-226. Chirala, S. S., W.Y. Huang, A. Jayakumar, K. Sakai, and S. J. Wakil. 1997. Animal fatty acid synthase: functional mapping and cloning and expression of the domain I constituent activities. Proc. Natl. Acad. Sci. U.S.A. 94:5588-5593. Chirala, S. S., H. Chang, M. Matzuk, L. Abu-Elheiga, J. Mao, K. Mahon, M. Finegold, and S. J. Wakil. 2003 Fatty acid synthesis is essential in embryonic development: fatty acid synthase null mutants and most of the heterozygotes die in utero. Proc. Natl. Acad. Sci. U.S.A. 100:6358-6363. Christ-Hazelof, E., and Nutgeren, D. 1979. Purification and characterisation of prostaglandin endoperoxide D-isomerase, a cytoplasmic, glutathione-requiring enzyme. Biochim. Biophys. Acta. 572:43-51. Cochary, E. F., Z. Kikinis, and K. E. Paulson. 1993. Positional and temporal regulation of lipogenic gene expression in mouse liver. Gene Expr. 3:265-277. Davis, G. P., and S. K. Denise. 1998. The impact of genetic markers on selection. J. Anim. Sci. 76:2331-2399. Diamond, R. H., K. Du, and V. M. Lee. 1993. Novel delayed-early and highly insulin-induced growth response genes: identification of HRS, a potential regulator of alternative pre-mRNA splicing. J. Biol. Chem. 268:15185-15192. Evans, M. I., P. J. O'Malley, A. Krust, and J. B. Burch. 1987. Developmental regulation of the estrogen receptor and the estrogen responsiveness of five yolk protein genes in the avian liver. Proc. Natl. Acad. Sci. U.S.A. 84:8493-8497. Fukuda, H., A. Katsurada, and N. Iritani. 1992. Nutritional and hormonal regulation of mRNA levels of lipogenic enzymes in primary cultures of rat hepatocytes. J. Biochem. 111:25-30. Giansanti, F., P. Rossi, M. T. Massucci, D. Botti, G. Antonini, P. Valenti, and L. Seqanti. 2002. Antiviral activity of ovotransferrin discloses an evolutionary strategy for the defensive activities of lactoferrin. Biochem. Cell Biol. 80:125-130. Giansanti, F., M. F. Giardi, M. T. Massucci, D. Botti, and G. Antonini.2007. Ovotransferrin expression and release by chicken cell lines infected with Marke's disease virus. Biochem. Cell Biol. 85:150-155. Glickman, R. M., M. Rogers, and J. N. Glickman. 1986. Apolipoprotein B synthesis by human liver and intestine in vitro. Proc. Natl. Acad. Sci. U.S.A. 83:5296-5300. Goldstein, J. L., R. A. DeBose-Boyd, and M. S. Brown. 2006. Protein sensors for membrane sterols. Cell 124:35-46. Gwee, P. C., K. Tang, J. M. Chua, E. J. Lee, S. S. Chong, and C. G. Lee. 2003. Simultaneous genotyping of seven single-nucleotide polymorphisms in the MDR1 gene by single-tube multiplex minisequencing. Clin. Chem. 49:672-676. Henegariu, O., P. Hirschman, K. Killian, C. Kirsch, R. Lengauer, and K. Maiwald. 1994. Rapid screening of the Y chromosome in idiopathic sterile men, diagnostic for deletions in AZF, agenetic Y factor expressed during spermatogenesis. Andrologia 26:97-106. Hermier, D., P. Forgez, and M. J. Chapman. 1985. A density gradient study of the lipoprotein and apolipoprotein distribution in the chicken, Gallus domesticus. Biochim. Biophys. Acta. 836:105-118. Hua, X., A. Norturfft, J. L. Goldstein, and M.S. Brown. 1996. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage activating protein. Cell 87:415-426. Hayash, K. 1991. PCR-SSCP: A simple and sensitive method for detection of mutations the genomic DNA. PCR Methods. Appl. 1:34-38. Innerarity, T. L., J. Bor, S. Yamanaka, and S. O. Olofsson. 1996. Biosynthesis of apolipoprotein B48-containing lipoproteins. J. Biol. Chem. 271:2353-2356. Iritani, N., N. Nishimoto, A. Katsurada, and H. Fukuda. 1992. Regulation of hepatic lipogenic enzyme gene expression by diet quantity in rats fed a fat-free: high carbohydrate diet. J. Nutl. 122:28-36. Ishigaki, S., M. Abramovita, and I. Listowsky. 1989. Glutathione-S-transferases are major cytosolic thyroid hormone binding proteins. Arch. Biochem. Biophys. 273:265-72. Iwase, H., and K. Hotta. 1977. Ovotransferrin subfractionation dependent upon carbohydrate chain differences. J. Biol. Chem. 252:5437-5443. Jackson, R. L., H. Y. Lin, L. Chan, and A. R. Means. 1977. Amino acid sequence of a major apoprotein from hen plasma very low density lipoproteins. J. Biol. Chem. 252:250-253. Jacquinot, P. M., D. Leger, J. M. Wieruszeski, B. Coddeville, J. Montreuil, and G. Spik. 1994. Change in glycosylation of chicken transferrin glycans biosynthesized during embryogenesis and primary culture of embryo hepatocytes. Glycobiology 4:617-624. Jayakumar, A., M. H. Tai, W. Y. Huang, W. al-Feel, M. Hsu, L. Abu-Elheiga, S. S. Chirala, and S. J. Wakil. 1995. Human fatty acid synthase: properties and molecular cloning. Proc. Natl. Acad. Sci. U.S.A. 92:8695-8699. Jeltsch, J. M., R. Hen, L. Maroteaux, J. M. Garnier, and P. Chambon. 1987.Sequence of the chicken ovotransferrin gene. Nucleic Acids Res. 15:7643-7645. Johanna, S., F. Silvia, L. Antti, and S. Antti. 2005. CYP2D6 Genotypeing by a multiplex primer extension reaction. Clin. Chem. 51:1291-1295. Kameda, K., and A.G. Goodridge. 1991. Isolation and partial characterization of the gene for goose fatty acid synthase. J. Biol. Chem. 266:419-426. Kameda, K. 1995. Thyroid hormone inhibits fatty acid synthase gene transcription in chicken liver. Mol. Cell. Biochern. 144:105-108. Katsurada, A., N. Iritani, H. Fukuda, Y. Matsuruma, N. Nishimoto, T. Noguchi, and T. Tanaka. 1990. Effects of nutrients and hormones on transcriptional and post-transcriptional regulation of fatty acid synthase in rat liver. Eur. J. Biochem. 190:427-433. Kimption C. P., D. Fisher, S. Watson, M. Adams. A. Urquhart, J. Lygo, and P. Gill. 1994. Evaluation of an automated DNA profiling system employing multiplex amplification of four terameric STR loci. Int. J. Legal Med. 106:302-311. Knaapen, A. M., H. B. Ketelslegers, R. W. Gottschalk, R. G. Janssen, A. D. Paulussen, H. J. Smeets, R. W. Godschalk, F. J. Van Schooten, J. C. Kleinjans, and J. H. Van Delft. 2004. Simultaneous genotyping of nine polymorphisms in xenobiotic-metabolizng enzymes by multiplex PCR amplification and single base extension. Clin. Chem. 50:1664-1668. Knott, T. J., R. J. Pease, L. M. Powell, S. C. Wallis, S. C. Rall, Jr., T. L. Inneratity, B. Blackhart, W. H. Taylor, Y. Marcel, R. Milne, D. Johnson, M. Fuller, A. J. Lusis, B. J. McCarthy, R. W. Mahley, B. Levy-Wilson, and J. Scott. 1986. Complete protein sequence and identification of structural domains of human apolipoprotein B. Nature 323:734-738. Kok, K., L. Snippe, G.Ab, and M. Gruber. 1985. Nucleasehypersensitive sites in chromatin of the estrogen-inducible apoVLDL II gene of chicken. Nucleic Acids Res. 13:5189-5202. Krapivner, S., E. Chernogubova, M. Ericsson, C. A. Glader, A. Hamsten, and F. M. van't Hooft. 2007. Human evidence for the involvement of insulin-induced gene 1 in the regulation of plasma glucose concentration. Diabetologia 50:94-102. Krzysztof, P. H., W. Liu, and G. G. Hammes. 1989. Molecular cloning and sequencing of chicken liver fatty acid synthase cDNA. Proc. Natl. Acad. Sci. 86:4387-4391. Laux, T., and M. Schweizer. 1990. Dietary-induced pre-translational control of rat fatty acid synthase. Biochem. J. 266:793-797. Lazier, C. B., S. A. Nadin-Davix, A. Elbrecht, M. L. Blue, and D. L. Williams. 1981. Estrogen receptor and the development of estrogenic responses in embryonic chick liver. Adv. Exp. Med. Biol. 138:19-38. Lemieux, S. 1997. Genetic susceptibility to visceral obesity and related clinical implications. Int. J. Obes. Relat. Metab. Disord. 21:831-838. Li, H., N. Deeb, H. Zhou, C. M. Ashwell, and S. J. Lamont. 2005. Chicken quantitative trait loci for growth and body composition associated with the very low density apolipoprotein-Ⅱ gene. Poult. Sci. 84:697-703. Luong, A. 2000. Identification of three novel SREBP-activated target genes: acetyl CoA synthetase, 3-β-hydroxysterol dehydrogenase, and CL-6/INSIG1. PhD thesis, Southwestern Medical Center, University of Texas at Dallas, pp 1-137. Luskey, K. L., M. S. Brown, and J. L. Goldstein. 1974. Stimulation of the synthesis of very low density lipoproteins in rooster liver by estradiol. J. Biol. Chem. 249:5939-5947. Makoto, N., T. An, L. Chan, N. O. Davidson, and B. B. Teng. 1999. Sequence elements required for apolipoprotein B mRNA editing enhancement activity from chicken enterocytes. Biochen. Biophys. Res. Commun. 254:744-750. Mannervik, B., Y. C. Awasthi, P. G. Board, J. D. Hayes, C. Di Ilio, B. Ketterer, I. Listowsky, R. Morgenstern, M. Muramatsu, and W. R. Pearson. 1992. Nomenclature for human glutathione transferases. Biochem. J. 282:305-306. Mannervik, B., P. Alin, C. Guthenberg, H. Jensson, M. K. Tahir, M. Warholm, and H. Jörnvall. 1985. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties. Proc. Natl. Acad. Sci. U.S.A. 82:7202-7206. Marrube, G., F. Rozen, G. B. Pinto, N. Pacienza, J. E. Melo, M. J. Huguet, Z. Canet, R. Zandomeni, and M. C. Miquel. 2004. New polymorphism of FASN gene in chicken. J. Appl. Genet. 45:453-455. Metz-Boutigue, M. H., J. Jollès, J. Mazurier, F. Schoentgen, D. Legrand, G. Spik, J. Montreuil, and P. Jolles. 1984. Human lactotransferrin: aminoacid sequence and structural comparisons with other transferrin. Eur. J. Biochem. 145:659-676. Meyer, D. J., B. Coles, S. E. Pemble, K. S. Gilmore, G. M. Fraser, and B. Ketterer. 1991. Theta, a new class of glutathione transferases purified from rat and man. Biochem. J. 274:409-414. Meyer, D. J., and M. Thomas. 1995. Characterization of rat spleen prostaglandin H D-isomerase as a sigma-class GSH transferase. Biochem. J. 311:739-742. Mohn, K. L., T. M. Laz, J. C. Hsu, A. E. Melby, R. Bravo, and R. Taub. 1991. The immediate-early growth response in regenerating liver and insulin-stimulated H-35 cells: comparison with serum-stimulated 3T3 cells and identification of 41 novel immediate-early genes. Mol. Cell. Biol. 11:381-390. Morgenstern, R., J. W. DePierre, and H. Jörnvall. 1985. Microsomal glutathione transferase: Primary structure. J. Biol. Chem. 260:13976-13983. Morgan, R. W., L. Sofer, A. S. Anderson, E. L. Bernberg, J. Cui, and J. Burnside. 2001. Induction of host gene expression following infection of chicken embryo fibroblasts with oncogenic Marek's disease virus. J. Virol. 75:533-539. Morris, S. M., J. H. Jr. Nilson, R. A, Jenik, L. K. Winbeny, M. A. Mcdevitt, and A. G. Goodridge. 1982. Molecular cloning of gene sequences for avian fatty acid synthase and evidence for nutritional regulation of fatty acid synthase and mRNA concentration. J. Biol. Chern. 257:3225-3229. Musa, H. H., G. H. Chen, and B. C. Li. 2007. The effect of interaction between lipoprotein lipase and apoVLDL-Ⅱ gene on fat and serum biochemical levels. Afr. J. Biotechnol. 6:847-852. Nathalie, L. F., E. K. Catherine, S. P. Rohan, D. Christian, M. Jacques, and D. Madeleine. 1996. Characterization of the chicken fatty acid synthase gene 5'part and promoter region. Eur. J. Biochem. 240: 323-330. Nimp, f., J., W. J. Schneider. 1991. Receptor-mediated lipoprotein transport in the laying hen. J. Nutr. 121:1471-1474. Noteborn, M. H., O. Bakker, M. A. de Jonge, M. Gruber, and G. Ab. 1986. Differnential estrogen responsiveness of the vitellgenin and apo very low density lipoprotein Ⅱ genes in the rooster liver. J. Steroid Biochem. 24: 281-285. Pati, N., V. Schowinsky, O. Kokanovic, V. Magnuson, and S. Ghosh. 2004. A comparison between SNaPshot, pyrosequencing, and biplex invader SNP genotyping methods: accuracy, cost, and throughput. J. Biochem. Biophys. Methods 60:1-12. Paulauskis, J. D., and H. S. Sul. 1989. Hormonal regulation of mouse fatty acid synthase gene transcription in liver. J. Biol. Chem. 264:574-577. Pemble, S. E., A. F. Wardle, and J. B. Taylor. 1996. Glutathione S-transferase class Kappa: characterization by the cloning of rat mitochondrial GST and identification of a human homologue. Biochem. J. 319:749-754. Ponce, M. R., P. Robles, and J. L. Micol. 1999. High-throughput genetic mapping in Arabidopsos thaliana. Mol. Gen. Genet. 261:408-415. Rieder, M. J., S. J. Taylor, V. O. R. Tobe, and D. A. Nickerson. 1998. Automating the identification of DNA variations using quality-based fluorescence re-sequencing: analysis of the human mitochondrial genome. Nucleic Acids Res. 26:967-973. Rosenfield, S. I., and L. A. Jaykus. 1999. A multiplex reverse transcription polymerase chain reaction method for the detection of foodborn viruses. J. Food Prot. 62:1210-1214. Roy, R., L. Ordovas, P. Zaragoza, A. Romero, C. Moreno, J. Altarriba, and C. Rodellar. 2006. Association of polymorphisms in the bovine FASN gene with milk-fat content. Anim. Genet. 37:215-218. Roy, R., S. Taourit, P. Zaragoza, A. Eggen, and C. Rodellar. 2005. Genomic structure and alternative transcript of bovine fatty acid synthase gene (FASN): comparative analysis of the FASN gene between monogastric and ruminant species. Cytogenet. Genome Res. 111:65-73 Semenkovich, C. F., T. Coleman, and R. Goforth. 1993. Physiologic concentrations of glucose regulate fatty acid synthase activity in HepG2 cells by mediating fatty acid synthase mRNA stability. J. Biol. Chern. 268:6961-6970. Semenkovich, C. F., T. Coleman, and F. T. Jr. Fiedorek. 1995. Human fatty acid synthase mRNA: tissue distribution, genetic mapping, and kinetics of decay after glucose deprivation. J. Lipid Res. 36:1507-1521. Schoske, R., P. M. Vallone, C. M. Ruitberg, and J. M. Butler, 2003. Multiplex PCR design strategy used for the simultaneous amplification of 10Y chromosome short tandem repeat (STR) loci. Anal. Bioanal. Chem. 375:333-343. Schumaker, V. N., M. L. Phillips, and J. E. Chatterton. 1994. Apolipoprotein B and low density lipoprotein structure: Implications for biosynthesis of triglyceride-rich lipoproteins. Adv. Protein Chem. 45:205-248. Shuber, A. P., J. Skoletsky, R. Stern, and B. L. Handelin. 1993. Efficient 12-mutation testing in the CFTR gene: a general model for complex mutation analysis. Hum. Mol. Genet. 2:153-158. Shuler, F. D., W. W. Chu, S. Wang, and M. I. Evans. 1998. A composite regulatory element in the first intron of the estrogen-responsive very low density apolipoprotein II gene. DNA Cell Biol. 17:689-697. Smidt, M. P., J. Wijnholds, L. Snippe,G.Van Keulen, and G. AB. 1994. Binding of a bZip protein to the estrogen-inducible apoVLDL II promoter. Biochim. Biophys. Acta 1219:115-120. Sourdioux, M., M. Douaire, and Y. Delabrosse. 1996. DNA polymorphism of lipogenesis genes and analysis of linkage with fatness in turkey. Poult. Sci. 75:1018-1026. Sourdioux, M., C. Brevelet, Y. Delabrosse, and M. Douaire. 1999. Association of fatty-acid synthase gene and malic enzyme gene polymorphism with fatness in turkey. Poult. Sci. 78:1651-1657. Stapleton, S. R., D. A. Mitchell, L. M. Salati, and A. G. Goodridge. 1990. Triiodothyronine stimulates transcription of the fatty acid synthase gene in chick embryo hepatocytes in culture. J. Biol. Ckem. 265:18442-18446. Stevens, L. 1991. Egg white proteins. Comp. Biochem. Physiol. B. 100:1-9. Stratil, A., L. Peelman, M. Van Poucke, and S. A. Cepica. 1997. A HinfⅠPCR-RFLP at the pocine leptin (LEP) gene. Anim. Genet. 28:371-372. Todd, G. K., C. Heinzmann, K. L. Svenson, D. A. Gordon, M. Nicosia, H. G. Lebherz, A. J. Lusis, and D. L. Williams. 1987. Requlation of chicken apolipoprotein B: Cloning and tissue distribution, and estrogen induction of mRNA. Gene 59:241-251. van het Schip, A. D., F. C. P. W. Meijlink, R. Strijker, M. Gruber, A. J. Van Vliet, J. A. M. Van De Kludert, and G. Ab. 1983. The nucleotide sequence of the chicken apo very low density lipoprotein Ⅱ gene. Nucleic Acids Res. 11:2529-2540. van het Schip, F., R. Strijker, J. Samallo, M. Gruber, and G. Ab. 1986. Conserved sequence motifs upstream from the co-ordinately expressed vitellogenin and apoVLDLII genes of chicken. Nucleic Acids Res. 14:8669-8680. Wakil, S. J., J. K. Stoops, and V. C. Joshi. 1983. Fatty acid synthesis and its regulation. Annu. Rev. Biochem. 52:537-579. Wieringa, B., W. Roskam, A. Arnberg, J. Vander Zwaag-Gerrit-sen, G. Ab, and M. Bruber. 1979. Purification of the mRNA for chicken very low density lipoprotein Ⅱ and molecular cloning or its full-length double-stranded cDNA. Nucleic Acids Res. 7:2147-2163. Wijnholds, J., E. Muller, and G. Ab. 1990. Oestrogen facilitates the binding of ubiquitous and liver-enriched nuclear proteins to the apoVLDL II promoter in vivo. Nucleic Acids Res. 19:33-41. Wijnholds, J., J. N. J. Phillipsen, and G.Ab. 1988. Tissue-specific and steroid-dependent interaction of transcription factors with the oestrogen-inducible apoVLDL II promoter in vivo. EMBO J. 7:2757-2763. Williams, J., T. C. Elleman, I. B. Kingstion, A. G. Wilkins, and K. A. Kuhn. 1982. The primary structure of hen ovotransferrin. Eur. J. Biochem. 122:297-303. Wilson, S. B., D. W. Back, S. M. Morris, J. Swierczynski, and A. G. Goodridge. 1986. Hormonal regulation of lipogenic enzymes in chick embryo hepatocytes in culture. J. Biol. Chern. 261:15179-15182. Wiskocil, R., P. Bensky, W. Dower, R. F. Goldberger, J. I. Gordon, and R.G. Deely. 1980. Coordinate regulation of two estrogen-dependent genes in avian liver. Proc. Natl. Acad. Sci. USA 77:4474-4478. Yang, T., J. L. Goldstein, and M. S. Brown. 2000 Overexpression of membrane domain of SCAP prevents sterols from inhibiting SCAP. SREBP exit from endoplasmic reticulum. J. Biol. Chem. 275:29881-29886. Yang, T., P. J. Espenshade, and M. E. Wright et al. 2002. Crucial step in cholesterol homeostasis: sterols promote binding of SCAP to INSIG-1, a membrane protein that facilitates retention of SREBPs in ER. Cell 110:489-500. Zhang, S., H. Li, and H. Shi. 2006. Single marker and haplotype analysis of the chicken apolipoprotein B gene T123G and D9500D9- polymorphism reveals association with body growth and obesity. Poult. Sci. 85:178-184.
本試驗之目的為利用土雞肝臟組織差異表現基因來探討差異表現基因之基因多態性。以PCR單股構形多態性分析法(single-strand conformation polymorphisms, PCR-SSCP)找出差異表現基因之基因態樣。麩胱苷肽轉移酶CL3次單元(glutathion S-transferase CL3 subunit, GST CL3)、卵運鐵蛋白酶(ovotransferrin, Otrf)、脂肪酸合成酶(fatty acid synthase, FASN)、胰島素誘導基因1(insulin induced gene 1, INSIG1)、脫輔基蛋白Ⅱ(apolipoprotein VLDLⅡ, apoVLDLⅡ)及脫輔基蛋白B(apoprotein B, apoB)等基因於聚丙烯醯胺膠片中皆呈現基因多態性。利用序列分析法偵測差異表現基因之SNP點。以PCR-RFLP及微序列分析法(minisequencing method)進行SNP之確認,所得結果一致。利用微序列法分析6個差異表現基因之SNP基因型並計算基因型頻率。發現Otrf基因在L2品系(G70C)、(T106C)、(C161T)及(A269G)變異皆為GG/TT/CC/GG之純合型。FASN基因(C41T)及apoVLDLⅡ基因(C93T)SNP點在L2與B品系皆以CC型最多。apoB1基因(T42C)在L2品系II型較ID及DD型為多,顯示這些結果為產蛋優勢之基因型。進一步以基因型與性狀資料進行統計分析之結果顯示,L2品系土雞之GST CL3 基因(T221C)之TT型雞之總產蛋數顯著高於TC及CC兩型者(P < 0.05)。apoVLDLⅡ基因(C93T),其CC型雞在2及4周齡體重高於CT型者(P < 0.01)。apoB1基因II型對之平均初產日高於ID及DD型者(P < 0.05)。以上試驗結果可作為土雞育種上候選基因之參考。

The aim of this study was to inverstigate the single nucleotide polymorphisms of the different expression genes in Taiwan country chicken livers. PCR-SSCP was used to find the gene polymorphisms and mutations. Glutathione S-transferase CL3 subunit (GST-CL3), ovotransferrin (Otrf), fatty acid synthase (FASN), insulin induced gene 1 (INSIG1), apolipoprotein VLDLⅡ (apoVLDLⅡ), and apoprotein B (apoB) genes were all found polymorphisms by PCR-SSCP. Sequencing was performed to find the SNPs. The genotypes obtained from PCR-RFLP were the same as minisequencing. The SNPs genotyping and frequency analysis of six differentially expressed genes in livers were carried out. The genotypes of Otrf (G70C), (T106C), (C161T) and (A269G) gene in L2 line were all GG/TT/CC/GG homozygotes. Most CC genotype of FASN (C41T) and apoVLDLⅡ (C93T) can be detected in both L2 and B lines. The frequency of genotype II of apoB1 (T42C) gene was more than the genotype ID and DD in the L2 line. It revealed that the genotype II of apoB1 (T42C) was more advantage for the reproductive traits especially significant on the 1st egg day. The relationship between genotypes and traits were investigated. The results showed that the total number eggs of TT type of GST CL3 (T221C) gene were higher than the TC and CC types in L2 line significantly (p < 0.05). The body weight at 2 and 4 wks of CC type of apoVLDLⅡ (C93T) gene was heavier than the type CT (p < 0.01). The age at 1st egg of apoB1 type II was significant early than the type ID and DD (p < 0.05). These results could provide the information for Taiwan country chicken selection in Taiwan.
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