Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/25112
標題: 台灣土雞脂肪組織差異表現轉錄體多態性與生產性狀之相關性
Association of Polymorphisms of Differentially Expressed Transcripts in Adipose Tissue of Taiwan Country Chickens with Performances
作者: 李淑嫺
Li, Shu-Hsien
關鍵字: Adipose tissue;脂肪組織;Laying traits;SNPs;Taiwan country chickens;產蛋性狀;SNPs;台灣土雞
出版社: 動物科學系所
引用: 白火城、黃森源、林仁壽。1996。家畜臨床血液生化學。立宇出版社。台南。第15-17頁。 李淵百、江碧玲、黃暉煌。1997。臺灣土雞最適上市週齡之研究。中畜會誌 26:285-296。 Afonso, S., L. Romagnano, and B. Babiarz. 1997. The expression and function of cystatin C and cathepsin B and cathepsin L during mouse embryo implantation and placentation. Development 124: 3415-3425. Akiyama, S. K., S.S. Yamada,and K. M. Yamada. 1986. Characterization of a 140-kD avian cell surface antigen as a fibronectin-binding molecule. J. Cell Biol. 102: 442-448. Alessi, D. R., M. Deak, A. Casamayor, F. B. Caudwell, N. Morrice, D. G. Norman, P. Gaffney, C. B. Reese, C. N. MacDougall, D. Harbison, A. Ashworth, and M. Bownes. 1997. 3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase. Curr. Biol. 7: 776-789. Anastasi A., M. A. Brown, A. A. Kembhavi, M. J. Nicklin, C. A. Sayers, D. C. Sunter, and A. J. Barrett. 1983. Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum. Biochem. J. 211: 129-138. Arosio, P., T. G. Adelman, and J. W. Drysdale. 1978. On ferritin heterogeneity. Further evidence for heteropolymers. J. Biol. Chem. 253: 4451-4458. Aziz, N., and H. N. Munro. 1986. Both subunits of rat liver ferritin are regulated at a translational level by iron induction. Nucleic. Acids Res. 14: 915-927. Banér, J., M. Nilsson, M. Mendel-Hartvig, and U. Landegren. 1998. Signal amplification of padlock probes by rolling circle replication. Nucleic Acids Res. 26: 5073-5078. Bar, A., E. Vax, and S. Striem. 1999. Relationships among age, eggshell thickness and vitamin D metabolism and its expression in the laying hen. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 123: 147-154. Barrett, A. J., H. Fritz, A. Grubb, S. Isemura, M. Järvinen, W. Machleidt, W. Müller-Esterl, M. Sasaki, and V. Turk. 1986. Nomenclature and classification of the proteins homologous with the cysteine-proteinase inhibitor chicken cystatin. Biochem. J. 236: 312. Bennett, R. R., J. den Dunnen, K. F. O''Brien, B. T. Darras, and L. M. Kunkel. 2001. Detection of mutations in the dystrophin gene via automated DHPLC screening and direct sequencing. BMC Genet. 2: 17. Björklund, S., G. Almouzni, I. Davidson, K. P. Nightingale, and K. Weiss. 1999. Global transcription regulators of eukaryotes. Cell 96: 759-767. Brand, H. S., U. H. Lerner, A. Grubb, W. Beertsen, A. V. Nieuw Amerongen, and V. Everts. 2004. Family 2 cystatins inhibit osteoclast-mediated bone resorption in calvarial bone explants. Bone. 35: 689-696. Brockway, B. E., and R. B. Freedman. 1984. Protein disulphide-isomerase of chick-embryo tendon. Biochem. J. 219: 51-59. Bryan, J. 1988. Gelsolin has three actin-binding sites. J. Cell Biol. 106:1553-1562. Buck, C. A., and A. F. Horwitz. 1987. Cell surface receptors for extracellular matrix molecules. Annu. Rev. Cell Biol. 3: 179-205. Chan, L., R. L. Jackson, B. W. O''Malley, and A. R. Means. 1976. Synthesis of very low density lipoproteins in the cockerel. Effects of estrogen. J. Clin. Invest. 58: 368-379. Chmurzyńska, A. 2006. The multigene family of fatty acid-binding proteins (FABPs): function, structure and polymorphism. J. Appl. Genet. 47: 39-48. Coe, N. R., M. A. Simpson, and D. A. Bernlohr. 1999. Targeted disruption of the adipocyte lipid-binding protein (aP2 protein) gene impairs fat cell lipolysis and increases cellular fatty acid levels. J. Lipid Res. 40: 967-972. Colella, R., Y. Sakaguchi, H. Nagase, and J. W. Bird. 1989. Chicken egg white cystatin. Molecular cloning, nucleotide sequence, and tissue distribution. J. Biol. Chem. 264: 17164-17169. Collart, M. A., and K. Struhl. 1993. CDC39, an essential nuclear protein that negatively regulates transcription and differentially affects the constitutive and inducible HIS3 promoters. EMBO J. 12: 177-186. Corbi, A. L., L. J. Miller, K. O''Connor, R. S. Larson, and T. A. Springer. 1987. cDNA cloning and complete primary structure of the alpha subunit of a leukocyte adhesion glycoprotein, p150,95. EMBO J. 6: 4023-4028. Cui, J. X., H. L. Du, Y. Liang, X. M. Deng, N. Li, and X. Q. Zhang. 2006. Association of polymorphisms in the promoter region of chicken prolactin with egg production. Poult. Sci. 85: 26-31. De Lorenzo, F., R. F. Goldberger, E. Jr. Steers, D. Givol, and B. Anfinsen. 1966. Purification and properties of an enzyme from beef liver which catalyzes sulfhydryl-disulfide interchange in proteins. J. Biol. Chem. 241: 1562-1567. Devarenne, T. P., S. K. Ekengren, K. F. Pedley, and G. B. Martin. 2006. Adi3 is a Pdk1-interacting AGC kinase that negatively regulates plant cell death. EMBO J. 25: 255-265. Draper, M. P., H. Y. Liu, A. H. Nelsbach, S. P. Mosley, and C. L. Denis. 1994. CCR4 is a glucose-regulated transcription factor whose leucine-rich repeat binds several proteins important for placing CCR4 in its proper promoter context. Mol. Cell Biol. 14: 4522-4531. Dugaiczyk, A., A. S. Inglis, P. M. Strike, R. W. Burley, W. G. Beattie, and L. Chan. 1981. Comparison of the nucleotide sequence of cloned DNA coding for an apolipoprotein (apo VLDL-II) from avian blood and the amino acid sequence of an egg-yolk protein (apovitellenin I): equivalence of the two sequences. Gene. 14: 175-182. Dunn, I. C., Y. W. Miao, A. Morris, M. N. Romanov, P. W. Wilson, D. Waddington. 2004. A study of association between genetic markers in candidate genes and reproductive traits in one generation of a commercial broiler breeder hen population. Heredity 92: 128-134. Ellegren, H. 2005. The avian genome uncovered. Trends Ecol. Evol. 220:180-186. Ferreira, C., D. Bucchini, M. E. Martin, S. Levi, P. Arosio, B. Grandchamp, and C. Beaumont. 2000. Early embryonic lethality of H ferritin gene deletion in mice. J Biol. Chem. 275: 3021-3024. Fossum, K., and J. R. Whitaker, 1968. Ficin and papain inhibitor from chicken egg white. Arch Biochem. Biophys. 125: 367-375. Garbi, N., G. Hämmerling, and S. Tanaka. 2006. Interaction of ERp57 and tapasin in the generation of MHC class I-peptide complexes. Curr. Opin. Immunol. 19: 99-105. Gimelfarb, A., and R, Lande. 1994. Simulation of marker assisted selection in hybrid populations. Genet. Res. 63: 39-47. Gimelfarb, A., and R, Lande. 1995. Marker-assisted selection and marker-QTL associations in hybrid populations. Theor. Appl. Genet. 91: 522-528. Griffin, H. D., and M. M. Perry. 1985. Exclusion of plasma lipoproteins of intestinal origin from avian egg yolk because of their size. Comp. Biochem. Physiol. B. 82: 321-325. Hayashi, K., M. Orita, Y. Suzuki, and T. Sekiya. 1989. Use of labeled primers in polymerase chain reaction (LP-PCR) for a rapid detection of the product. Nucleic Acids Res. 17: 3605. Hermier, D. 1997. Lipoprotein metabolism and fattening in poultry. J. Nutr. 127: 805-808. Hinssen, H., J. Vandekerckhove, and E. Lazarides. 1987. Gelsolin is expressed in early erythroid progenitor cells and negatively regulated during erythropoiesis. J. Cell Biol. 105: 1425-1433. Horwitz, A., K. Duggan, R. Greggs, C. Decker, and C. Buck. 1985. The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin. J. Cell Biol. 101: 2134-2144. Howell, W. M., M Jobs, U. Gyllensten, and A. J. Brookes, 1999. Dynamic allele-specific hybridization. Nat. Biotechnol. 17: 87-88. Hynes, R. O., E. E. Marcantonio, M. A. Stepp, L. A. Urry, and G. H. Yee. 1989. Integrin heterodimer and receptor complexity in avian and mammalian cells. J. Cell Biol. 109: 409-420. Hynes, R. O. 1992. Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69: 11-25. Iwahana, H., K. Yoshimoto, N, Mizusawa, E. Kudo, and M. Itakura. 1994. Multiple fluorescence-based PCR-SSCP analysis. Biotechniques. 16: 296-297, 300-305. Jackson, R. L., H. Y. Lin, L. Chan, and A. R. Means. 1977. Amino acid sequence of a major apoprotein from hen plasma very low density lipoproteins. J. Biol. Chem. 252: 250-253. Jalving, R., R. van''t Slot, and B. A. van Oost. 2004. Chicken single nucleotide polymorphism identification and selection for genetic mapping. Poult. Sci. 83: 1925-1931. Koya, R. C., H. Fujita, S. Shimizu, M. Ohtsu, M. Takimoto, Y. Tsujimoto, and N .Kuzumaki. 2000. Gelsolin inhibits apoptosis by blocking mitochondrial membrane potential loss and cytochrome c release. J. Biol. Chem. 275: 15343-15349. Kwok, P. Y. 2000. High-throughput genotyping assay approaches. Pharmacogenomics. 1: 95-100. Lande, R., and R.Thompson. 1990. Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics. 124: 743-756. Larson, L., S. Arnaudeau, B. Gibson, W. Li, R. Krause, B. Hao, J. R. Bamburg, D. P. Lew, N. Demaurex, and F. Southwick. 2005. Gelsolin mediates calcium-dependent disassembly of Listeria actin tails. Proc. Natl. Acad. Sci. USA. 102: 1921-1926. Lei, M., C. Luo, X. Peng, M. Fang, Q. Nie, D. Zhang, G. Yang, and X. Zhang. 2007. Polymorphism of growth-correlated genes associated with fatness and muscle fiber traits in chickens. Poult. Sci. 86: 835-842. Li, H., N. Deeb, H. Zhou, C. M. Ashwell, and S. J. Lamont. 2005. Chicken quantitative trait loci for growth and body composition associated with the very low density apolipoprotein-II gene. Poult. Sci. 84: 697-703. Liu, H. Y., V. Badarinarayana, D. C. Audino, J. Rappsilber, M. Mann, and C. L. Denis. 1998. The NOT proteins are part of the CCR4 transcriptional complex and affect gene expression both positively and negatively. EMBO J. 17: 1096–1106. Lubritz, D. L., and J. L. Smith. 1996. Genetic Parameter Estimates for Egg Production in Dwarf Broiler Breeders. J. Appl. Poult. Res. 5: 305-310. Luo, G. F., J. L. Chen, J. Wen, G. P. Zhao, M. Q. Zheng, and S. D. Sun. 2006 Study of single nucleotide polymorphism of A-FABP gene and its association with fatness traits in chicken. Yi Chuan 28: 39-42. Livak, K.J. 1999. Allelic discrimination using fluorogenic probes and the 5’nuclease assay. Genet. Anal. 14: 143-149. Malashkevich, V. N., B. V. Strokopytov, V. V. Borisov, Z. Dauter, K. S. Wilson, and Y. M. Torchinsky. 1995. Crystal structure of the closed form of chicken cytosolic aspartate aminotransferase at 1.9 A resolution. J. Mol. Biol. 247: 111-124. Manukyan A, Zhang J, Thippeswamy U, Yang J, Zavala N, Mudannayake MP, Asmussen M, Schneider C, Schneider BL. 2008. Ccr4 Alters Cell Size in Yeast by Modulating the Timing of CLN1 and CLN2 Expression. Genetics 179: 345-357. Marsh, I. B., and R. J. Whittington. 2007. Genomic diversity in Mycobacterium avium: Single nucleotide polymorphisms between the S and C strains of M. avium subsp. paratuberculosis and with M. a. avium. Mol. Cell. Probes 21: 66-75. Molinari, M., and A. Helenius. 1999. Glycoproteins form mixed disulphides with oxidoreductases during folding in living cells. Nature. 402: 27-29. Mora, A., D. Komander, D. M. van Aalten, and D. R. Alessi. 2004. PDK1, the master regulator of AGC kinase signal transduction. Semin. Cell. Dev. Biol. 15: 161-170. Musa, H. H., and G. H. Chen. 2007. Association of polymorphisms in avian apo- VLDL-II gene with body weight and abdominal fat weight. Afr. J. Biotechnol. 6: 2009-2013. Musa, H. H., G. H. Chen, and B. C. Li. 2007. The effect of interaction between Lipoprotein Lipase and ApoVLDL-II genes on fat and serum biochemical levels. Afr. J. Biotechnol. 6: 847-852. Nathwani, R. A., S. Pais, T. B. Reynolds, and N. Kaplowitz. 2005. Serum alanine aminotransferase in skeletal muscle diseases. Hepatology 41: 380-382. Nemere, I., S. E. Safford, B. Rohe, M. M. DeSouza, and M. C. Farach-Carson. 2004a. Identification and characterization of 1,25D3-membrane-associated rapid response, steroid (1, 25D3-MARRS) binding protein. J. Steroid Biochem. Mol. Biol. 90: 281-285. Nemere, I., M. C. Farach-Carson, B. Rohe, T. M. Sterling, A. W. Norman, B. D. Boyan, and S. E. Safford. 2004b. Ribozyme knockdown functionally links a 1, 25(OH)2D3 membrane binding protein (1,25D3-MARRS) and phosphate uptake in intestinal cells. Proc. Natl. Acad. Sci. USA 101: 7392-7397. Nemere, I. 2005. The 1, 25D3-MARRS protein: contribution to steroid stimulated calcium uptake in chicks and rats. Steroids 70: 455-457. Nilsson, M., K. Krejci, J. Koch, M. Kwiatkowski, P. Gustavsson, and U. Landegren. 1997. Padlock probes reveal single-nucleotide differences, parent of origin and in situ distribution of centromeric sequences in human chromosomes 13 and 21. Nat. Genet. 16: 217-218. Nodes, B. R., J. E. Shackelford, and H. G. Lebherz. 1987. Synthesis and secretion of serum gelsolin by smooth muscle tissue. J. Biol. Chem. 262: 5422-5427. Nyrén, P., B. Pettersson, and M. Uhlén. 1993. Solid phase DNA minisequencing by an enzymatic luminometric inorganic pyrophosphate detection assay. Anal. Biochem. 208: 171-175. Ockner, R. K., and J. A. Manning. 1976. Fatty acid binding protein. Role in esterification of absorbed long chain fatty acid in rat intestine. J. Clin. Invest. 58: 632-641. Ohba, H., T. Harano, and T. Omura. 1977. Presence of two different types of protein-disulfide isomerase on cytoplasmic and luminal surfaces of endoplasmic reticulum of rat liver cells. Biochem. Biophys. Res. Commun. 77: 830-836. Orita, M., H. Iwahana, H. Kanazawa, K. Hayashi, and T. Sekiya. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA. 86: 2766-2770. Ovesná, J., K. Poláková, L. Kuăera, and J. Rulcová. 2003 SNP typing in cereals: comparison of SSCP and SnaPShot markers using the barley Mlo locus as a model. Czech. J. Genet. Plant Breed 39: 109-112. Panteghini, M., F. Pagani, and C. Cuccia. 1987. Activity of serum aspartate aminotransferase isoenzymes in patients with acute myocardial infarction. Clin. Chem. 33: 67-71. Richards, M. P., S. M. Poch, C. N. Coon, R. W. Rosebrough, C. M. Ashwell, and J. P. McMurtry. 2003. Feed restriction significantly alters lipogenic gene expression in broiler breeder chickens. J. Nutr. 133: 707-715. Roth, R. A., and M. E. Koshland. 1981. Role of disulfide interchange enzyme in immunoglobulin synthesis. Biochemistry 20: 6594-6599. Salvante, K. G., G. Lin, R. L. Walzem, and T. D. Williams. 2007. Characterization of very-low density lipoprotein particle diameter dynamics in relation to egg production in a passerine bird. J. Exp. Biol. 210: 1064-1074. Sanger, F., A. R.Coulson. 1975. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J. Mol. Biol. 94: 441- 448. Schneider, W. J., R. Carroll, D. L. Severson, and J. Nimpf. 1990. Apolipoprotein VLDL-II inhibits lipolysis of triglyceride-rich lipoproteins in the laying hen. J. Lipid Res. 31: 507-513. Schwartz, M. A., M. D. Schaller, and M. H. Ginsberg. 1995. Integrins: emerging paradigms of signal transduction. Annu. Rev. Cell Dev. Biol. 11: 549-599. Schwede, A., L. Ellis, J. Luther, M. Carrington, G. Stoecklin, and C.Clayton. 2008. A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells. Nucleic Acids Res. 1-15. Shimaoka, M., and T. A. Springer. 2003. Therapeutic antagonists and conformational regulation of integrin function. Nat. Rev. Drug Discov. 2: 703-716. Siddiqi, A. I., M. Siddiqeh, A. Mehmood, and A. M. Siddiqui. 2007. Alanine aminotransferase/aspartate aminotransferase ratio reversal and prolonged prothrombin time: a specific indicator of hepatic cirrhosis. J. Ayub. Med. Coll. Abbottabad. 19: 22-24. Spinardi, L., R. Mazars, and C. Theillet. 1991. Protocols for an improved detection of point mutations by SSCP. Nucleic Acids Res. 19: 4009. Springer, T. A. 1994. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76: 301-314. Springer, T. A. 1997. Folding of the N-terminal, ligand-binding region of integrin alpha-subunits into a beta-propeller domain. Proc. Natl. Acad. Sci. USA 94: 65-72. Stevens, P. W., J. B. Dodgson, and J. D. Engel. 1987. Structure and expression of the chicken ferritin H-subunit gene. Mol. Cell Biol. 7: 1751-1758. Sundström, H., M. T. Webster, and H. Ellegren. 2004. Reduced variation on the chicken Z chromosome. Genetics 167: 377-385. Takada, Y., X. Ye, and S. Simon. 2007. The integrins. Genome Biol. 8: 215. Tamkun, J. W., D. W. DeSimone, D. Fonda, R. S. Patel, C. Buck, A. F. Horwitz, and R. O. Hynes. 1986. Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin. Cell 46: 271-282. Taylor, J. G., E. H. Choi, C. B. Foster, and S. J. Chanock. 2001. Using genetic variation to study human disease. Trends Mol. Med. 7: 507-512. Theil, E. C., and G. M. Tosky. 1979. Red cell ferritin and iron storage during chick embryonic development. Dev. Biol. 69: 666-672. Töhönen, V., C. Österlund, and K. Nordqvist. 1998. Testatin: a cystatin-related gene expressed during early testis development. Proc. Natl. Acad. Sci. USA 95: 14208-14213. Tordjman, J., S. Leroyer, G. Chauvet, J. Quette, C. Chauvet, C. Tomkiewicz, C. Chapron, R. Barouki, C. Forest, M. Aggerbeck, and B. Antoine. 2007. Cytosolic aspartate aminotransferase, a new partner in adipocyte glyceroneogenesis and an atypical target of thiazolidinedione. J. Biol. Chem. 282: 23591-23602. Trziszka, T., Y. Saleh, W. Kopeæ, M. Siewiñski, and E. Wesierska. 2004. Effect of hen’s age on the level of cystatin in the chicken egg white. Int. J. Poult. Sci. 3: 471-477. van Hemert, S., A. J. Hoekman, M. A. Smits, and J. M. Rebel. 2007. Immunological and gene expression responses to a Salmonella infection in the chicken intestine. Vet. Res. 38: 51-63. Van Nieuwenhoven, F. A., G. J. Van der Vusse, and J. F. Glatz. 1996. Membrane-associated and cytoplasmic fatty acid-binding proteins. Lipids 31: 223-227 Veerkamp, J. H., and R. G. Maatman. 1995. Cytoplasmic fatty acid-binding proteins: their structure and genes. Prog. Lipid Res. 34: 17-52. Wallis, J., J. Aerts, M. Groenen, R. Crooijmans, D. Layman, T. Graves, D. Scheer, C. Kremitzki, M. Fedele, N. Mudd, M. Cardenas, J. Higginbotham, J. Carter, R. Mcgrane, T. Gaige, K. Mead, J. Walker, D. Albracht, J. Davito, S.-P. Yang, S. Leong, A. Chinwalla, M. Sekhon, K. Wylie, J. Dodgson, M. Romanov, H. Cheng, P. D. Jong, K. Osoegawa, M. Nefedov, H. Zhang, J. Mcpherson, M. Krzywinski, J. Schein, L. Hillier, E. Mardis, R. Wilson, and W. Warren. 2004. A physical map of the chicken genome. Nature 432: 761-764. Walzem, R. L., P. A. Davis, and R. J. Hansen. 1994. Overfeeding increases very low density lipoprotein diameter and causes the appearance of a unique lipoprotein particle in association with failed yolk deposition. J. Lipid Res. 35: 1354-1366. Wang, D. G., J. B. Fan, C. J. Siao, A. Berno, P. Young, R. Sapolsky, G. Ghandour, N. Perkins, E. Winchester, J. Spencer, L. Kruglyak, L. Stein, L. Hsie, T. Topaloglou, E. Hubbel, E. Robinson, M. Mittmann, M. S. Morris, N. Shen, D. Kilburn, J. Rioux, C. Nusbaum, S. Rozen, T. J. Hudson, R. Lipshutz, M. Chee, and E. S. Lander. 1998. Large-scale identification, mapping, and genotyping of single-nucleotide polymorphisms in the human genome. Science 280:1077-1082. Wang, Q., H. Li, N. Li, Z. Gu, and Y. Wang. 2004. Cloning and characterization of chicken adipocyte fatty acid binding protein gene. Anim. Biotechnol. 15: 121-132. Wang, Q., H. Li, N. Li, L. Leng, Y. Wang, and Z. Tang. 2006. Identification of single nucleotide polymorphism of adipocyte fatty acid-binding protein gene and its association with fatness traits in the chicken. Poult. Sci. 85:429-434. Weeds, A., and S. Maciver. 1993. F-actin capping proteins. Curr. Opin. Cell Biol. 5: 63-69. Whittaker, J. C., R. N. Curnow, C. S. Haley, and R. Thompson. 1995. Using marker-maps in marker-assisted selection. Genet. Res. 66: 255-265. Wieringa, B., W. Roskam, A. Arnberg, J. van der Zwaag-Gerritsen, G. Ab, and M. Gruber. 1979. Purification of the mRNA for chicken very low density lipoproteinII and molecular cloning of its full-length double-stranded cDNA. Nucleic Acids Res. 7: 2147-2163. Wille, A., and S. M. Leal. 2001. Novel selection criteria for genome scans of complex traits. Genet. Epidemiol. 21: 800-804. Williams, M. R., J. S. Arthur, A. Balendran, J. van der Kaay, V. Poli, P. Cohen, and D. R. Alessi. 2000. The role of 3-phosphoinositide-dependent protein kinase 1 in activating AGC kinases defined in embryonic stem cells. Curr. Biol. 10:439-448. Witke, W., A. H. Sharpe, J. H. Hartwig, T. Azuma, T. P. Stossel, and D. J. Kwiatkowski. 1995. Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin. Cell 81: 41-51. Yin, H. L., and T. P. Stossel. 1979. Control of cytoplasmic actin gel-sol transformation by gelsolin, a calcium-dependent regulatory protein. Nature 281: 583-586. Yin, H. L., J. H. Albrecht, and A. Fattoum. 1981. Identification of gelsolin, a Ca2+-dependent regulatory protein of actin gel-sol transformation, and its intracellular distribution in a variety of cells and tissues. J. Cell Biol. 91:901-906. Yin, Y., H. Yuan, C. Wang, N. Pattabiraman, M. Rao, R. G. Pestell, and R. I. Glazer. 2006. 3-phosphoinositide-dependent protein kinase-1 activates the peroxisome proliferator- activated receptor-gamma and promotes adipocyte differentiation. Mol. Endocrinol. 20: 268-278. Yao, J. F., Y. Zhang, G. Q. Wu, J. X. Zheng, X. M. Deng, and N, Yang. 2008. Polymorphisms of chicken apoA5 gene and association with carcass traits of chickens. Yi Chuan 30: 607-612. Zähringer, J., B. S. Baliga, and H. N. Munro. 1976. Novel mechanism for translational control in regulation of ferritin synthesis by iron. Proc. Natl. Acad. Sci. USA. 73: 857-861. Zhang, X.L., X. Jiang, Y. P. Liu, H. R. Du, and Q. Zhu. 2007. Identification of Avai polymorphisms in the third intron of GH gene and their associations with abdominal fat in chickens. Poult. Sci. 86: 1079-1083. Zimmerman, A. W., and J. H. Veerkamp. 1998. Members of the fatty acid-binding protein family inhibit cell-free protein synthesis. FEBS Lett. 437: 183-186.
摘要: 
本試驗之主要目的是針對台灣土雞晶片試驗脂肪組織之高低產差異表現轉錄基因建立SNPs(single nucleotide polymorphisms),並探討其與性狀表現之相關性。首先利用單股核酸構形多態性(single strand conformation polymorphism, SSCP)檢測AFABP、apoVLDLII、CST3、CNOT8、FTH1、GOT1、GSN、ITFG1、PDIA3以及PDPK1等差異表現基因片段,發現其結果皆具不同態樣,經序列分析結果,確認十個基因共21個變異位置。進一步設計特異引子,利用微序列分析法(minisequencing)對219隻L2品系與93隻B品系台灣土雞進行基因型分析。發現每種基因皆可以利用微序列檢測出不同基因型。最後將各基因型與性狀進行統計分析發現,AFABP之C332T與G412A處分別為CC及GG基因型者,其平均初產日齡的表現上顯著地較TT及AA基因型者為低(P < 0.05)。CST3之T97G、T139G、A186G、T236C與T267C處分別為TT、TT、AA、TT及TT基因型者,其總產蛋數與產蛋率顯著高於為GG、GG、GG、CC及CC基因型者(P < 0.05),而T97G之平均停產日則較短;再者,T236C與T267C為TC雜合型者之平均產蛋日與平均窩蛋數皆顯著大於CC型者(P < 0.05);而在體重方面T139G與A186G變異點為GG基因型者之體重表現亦顯著低於TT型及AA型者(P < 0.05),T236C與T267C為TT及TC基因型者之體重表現亦顯著高於CC型者(P < 0.05);GOT1-G27A之AA基因型者在平均產蛋日、平均窩蛋數及最大窩蛋數的表現上均顯著地大於GG型及GA型者(P < 0.05)。GSN基因缺少第173-175鹼基(-/-) 及C219T-TT基因型者,其第12至36週齡體重皆顯著高於另一純合型[+/+]及CC基因型(P < 0.05)。ITFG1基因之G78A,在產蛋性狀方面,GA基因型者之最大窩蛋數顯著多於GG型者。而在PDPK1之C268T,為CC基因型者於平均初產日齡及平均停產日均顯著小於TT型者(P < 0.05)。總結而得選拔AFABP為C332T-CC與G412A-GG基因型;CST3為T97G-TT、T139G-TT及A186G-AA基因型,或T236C及T267C之交替基因為T者;GOT1為G27A- AA基因型;ITFG1為G78A-GA基因型;或PDPK1為C268T-CC型者皆有利於生產性能之提升。而選拔CST3為T139G-TT與A186G-AA基因型,以及T236C與T267C之交替基因為T者;或GSN之nt173-175 (-/-)及C219T-TT型者則有利於生長表現之提升。此研究結果可應用於土雞的育種選拔計畫,使其更有快速改良效益。

The purpose of this study is to investigate the SNPs (single nucleotide polymorphisms) genotypes of differentially expressed genes in adipose tissue of Taiwan country chickens, and their correlations with laying and growth Traits. The SSCP (single strand conformation polymorphism) analysis of AFABP, apoVLDLII, CST3, CNOT8, FTH1, GOT1, GSN, ITFG1, PDIA3 and PDPK1 genes was performed. The different types of SSCP patterns in all these genes were found. Nucleotide sequencing was carried out. Twenty-one SNPs were observed in these 10 gene sequences. Genotyping of 219 L2 and 93 B lines of Taiwan country chickens, detected were carried by minisequencing. The different genotypes of these genes were presented in chickens. The correlations between genotypes and performances of chickens were investigated. The results showed that the age of first egg (AFE) of C332T-CC and G412A-GG in AFABP, are significantly earlier than TT and AA types (P < 0.05). While the T97G, T139G, A186G, T236C and T267C of CST3 gene are TT, TT, AA, TT and TT types respectively, the number of total eggs (Eggs) and hen day rate (HDR) are significantly higher than GG, GG, GG, CC and CC types (P < 0.05); Whereas, the mean pause day (MPD) of T97G-TT is significantly shorter than it of T97G-GG; Furthermore, when the T236C and T267C of CST3 are TC heterozygote, the mean lay day (MLD) and mean clutch egg (MCE) are significantly higher than that are CC genotype (P < 0.05); Body weight (BW) of T139G-TT and A186G-AA, T236C-TT/TC and T267C-TT/TC genotypes in CST3 are significantly higher than that of the other homozygotes. MLD, MCE and max clutch egg of G27A-AA type in GOT1 are significantly higher than those of GG and GA types (P < 0.05). The chickens with nt173-175 (-/-) and C219T-TT genotypes in GSN are significantly heavier than the chickens with nt173-175 (+/+) and C219T-CC genotypes for BW at 12-36 weeks (P < 0.05). Chickens with G78A-GA genotype of ITFG1 are significantly higher than those with GG type for max clutch egg (P < 0.05). Chickens with C268T-CC genotype of in PDPK1 are significantly shorter than those with TT type for AFE and MPD (P < 0.05). We conclude that C332T-CC and G412A-GG of AFABP; T97G-TT, T139G-TT and A186G-AA or allele T of T236C and T267C in CST3; G27A- AA of GOT1; G78A-GA of ITFG1; and C268T-CC of PDPK1, can be used in a selection program for develop of egg production. Furthermore, T139G-TT, A186G-AA and allele T of T236C and T267C in CST3; nt173-175 (-/-) and C219T-TT of GSN, acted in dominant fashion on growth performance of chickens. The results could be applied these genotype loci in the large scale chickens breeding improvement programs.
URI: http://hdl.handle.net/11455/25112
其他識別: U0005-3107200818592400
Appears in Collections:動物科學系

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